Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311883454> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W4311883454 abstract "Abstract Discrete subaortic stenosis (DSS) is a congenital heart disease characterized by the formation of a fibrotic membrane below the aortic valve. The underlying cellular mechanisms of this disease are currently unknown. As one of the distinguishing features of DSS is the elevated pressure gradient in the left ventricular outflow tract, it is theorized that the membrane formation is caused by elevated wall shear stress applied to the endocardial endothelial cells (EECs), triggering fibrosis. To relate shear stress to an EEC fibrotic phenotype, we applied fluid shear stress to EECs at physiological and pathological shear rates using a cone-and-plate device. Upon characterization of the EECs after the shear experiments, elevated shear stress triggered cell alignment as well as endothelial-to-mesenchymal transformation (EndMT) signaling pathways driven by upregulation of SNAI1 gene expression. The EECs were then treated with a small molecule inhibitor of Snail1 protein, CYD19, to attempt to attenuate EndMT signaling, and subsequently subjected to pathological shear stress. We found the Snail1 inhibitor did downregulate selected markers of EndMT signaling, although only transiently. Interestingly, the application of shear stress had a far greater effect on the EEC gene and protein expression in comparison to the Snail1 inhibition. Our findings are the first insight to EEC specific response to high shear stress. Further study should reveal the mechanisms that drive fibrosis and the formation of the DSS membrane." @default.
- W4311883454 created "2023-01-02" @default.
- W4311883454 creator A5006906927 @default.
- W4311883454 creator A5012208343 @default.
- W4311883454 creator A5013804716 @default.
- W4311883454 creator A5030988494 @default.
- W4311883454 creator A5044710928 @default.
- W4311883454 creator A5063596086 @default.
- W4311883454 creator A5076134748 @default.
- W4311883454 date "2022-12-08" @default.
- W4311883454 modified "2023-09-27" @default.
- W4311883454 title "Application of Physiological and Pathological Wall Shear Stress to Endocardial Endothelial Cells Triggers Complex Signaling Pathways" @default.
- W4311883454 cites W1820063374 @default.
- W4311883454 cites W1933152830 @default.
- W4311883454 cites W1993745515 @default.
- W4311883454 cites W1998002017 @default.
- W4311883454 cites W2040513651 @default.
- W4311883454 cites W2075421738 @default.
- W4311883454 cites W2106552550 @default.
- W4311883454 cites W2107946403 @default.
- W4311883454 cites W2336878672 @default.
- W4311883454 cites W2484216882 @default.
- W4311883454 cites W2555915815 @default.
- W4311883454 cites W2587625705 @default.
- W4311883454 cites W2612661993 @default.
- W4311883454 cites W2623383502 @default.
- W4311883454 cites W2734551513 @default.
- W4311883454 cites W2763146615 @default.
- W4311883454 cites W2789551841 @default.
- W4311883454 cites W2908974911 @default.
- W4311883454 cites W2931401907 @default.
- W4311883454 cites W2947769029 @default.
- W4311883454 cites W3008057530 @default.
- W4311883454 cites W3018126040 @default.
- W4311883454 cites W3093366451 @default.
- W4311883454 cites W3125236105 @default.
- W4311883454 cites W3126999737 @default.
- W4311883454 cites W3128540139 @default.
- W4311883454 cites W3174767295 @default.
- W4311883454 cites W3176328315 @default.
- W4311883454 cites W3197421733 @default.
- W4311883454 cites W4224287976 @default.
- W4311883454 cites W4226198419 @default.
- W4311883454 cites W4281720842 @default.
- W4311883454 doi "https://doi.org/10.1101/2022.12.05.519049" @default.
- W4311883454 hasPublicationYear "2022" @default.
- W4311883454 type Work @default.
- W4311883454 citedByCount "0" @default.
- W4311883454 crossrefType "posted-content" @default.
- W4311883454 hasAuthorship W4311883454A5006906927 @default.
- W4311883454 hasAuthorship W4311883454A5012208343 @default.
- W4311883454 hasAuthorship W4311883454A5013804716 @default.
- W4311883454 hasAuthorship W4311883454A5030988494 @default.
- W4311883454 hasAuthorship W4311883454A5044710928 @default.
- W4311883454 hasAuthorship W4311883454A5063596086 @default.
- W4311883454 hasAuthorship W4311883454A5076134748 @default.
- W4311883454 hasBestOaLocation W43118834541 @default.
- W4311883454 hasConcept C104317684 @default.
- W4311883454 hasConcept C126322002 @default.
- W4311883454 hasConcept C127561419 @default.
- W4311883454 hasConcept C159985019 @default.
- W4311883454 hasConcept C185592680 @default.
- W4311883454 hasConcept C192562407 @default.
- W4311883454 hasConcept C21141959 @default.
- W4311883454 hasConcept C2780559512 @default.
- W4311883454 hasConcept C55493867 @default.
- W4311883454 hasConcept C62478195 @default.
- W4311883454 hasConcept C71924100 @default.
- W4311883454 hasConcept C86803240 @default.
- W4311883454 hasConcept C95444343 @default.
- W4311883454 hasConceptScore W4311883454C104317684 @default.
- W4311883454 hasConceptScore W4311883454C126322002 @default.
- W4311883454 hasConceptScore W4311883454C127561419 @default.
- W4311883454 hasConceptScore W4311883454C159985019 @default.
- W4311883454 hasConceptScore W4311883454C185592680 @default.
- W4311883454 hasConceptScore W4311883454C192562407 @default.
- W4311883454 hasConceptScore W4311883454C21141959 @default.
- W4311883454 hasConceptScore W4311883454C2780559512 @default.
- W4311883454 hasConceptScore W4311883454C55493867 @default.
- W4311883454 hasConceptScore W4311883454C62478195 @default.
- W4311883454 hasConceptScore W4311883454C71924100 @default.
- W4311883454 hasConceptScore W4311883454C86803240 @default.
- W4311883454 hasConceptScore W4311883454C95444343 @default.
- W4311883454 hasLocation W43118834541 @default.
- W4311883454 hasOpenAccess W4311883454 @default.
- W4311883454 hasPrimaryLocation W43118834541 @default.
- W4311883454 hasRelatedWork W1969200465 @default.
- W4311883454 hasRelatedWork W1971018030 @default.
- W4311883454 hasRelatedWork W2068197222 @default.
- W4311883454 hasRelatedWork W2131613539 @default.
- W4311883454 hasRelatedWork W2171862581 @default.
- W4311883454 hasRelatedWork W2748952813 @default.
- W4311883454 hasRelatedWork W2899084033 @default.
- W4311883454 hasRelatedWork W2973133007 @default.
- W4311883454 hasRelatedWork W3091168493 @default.
- W4311883454 hasRelatedWork W4210757985 @default.
- W4311883454 isParatext "false" @default.
- W4311883454 isRetracted "false" @default.
- W4311883454 workType "article" @default.