FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4311914314> ?p ?o ?g. }

• W4311914314 endingPage "e0270317" @default.
• W4311914314 startingPage "e0270317" @default.
• W4311914314 abstract "Key targets of both the therapeutic and abused properties of opioids are μ-opioid receptors (MORs). Despite years of research investigating the biochemistry and signal transduction pathways associated with MOR activation, we do not fully understand the cellular mechanisms underlying opioid addiction. Given that addictive opioids such as morphine, oxycodone, heroin, and fentanyl all activate MORs, and current therapies such as naloxone and buprenorphine block this activation, the availability of tools to mechanistically investigate opioid-mediated cellular and behavioral phenotypes are necessary. Therefore, we derived, validated, and applied a novel MOR-specific Cre mouse line, inserting a T2A cleavable peptide sequence and the Cre coding sequence into the MOR 3'UTR. Importantly, this line shows specificity and fidelity of MOR expression throughout the brain and with respect to function, there were no differences in behavioral responses to morphine when compared to wild type mice, nor are there any alterations in Oprm1 gene expression or receptor density. To assess Cre recombinase activity, MOR-Cre mice were crossed with the floxed GFP-reporters, RosaLSLSun1-sfGFP or RosaLSL-GFP-L10a. The latter allowed for cell type specific RNA sequencing via TRAP (Translating Ribosome Affinity Purification) of striatal MOR+ neurons following opioid withdrawal. The breadth of utility of this new tool will greatly facilitate the study of opioid biology under varying conditions." @default.
• W4311914314 created "2023-01-02" @default.
• W4311914314 creator A5012195642 @default.
• W4311914314 creator A5013450521 @default.
• W4311914314 creator A5017805012 @default.
• W4311914314 creator A5018638118 @default.
• W4311914314 creator A5022907334 @default.
• W4311914314 creator A5046541978 @default.
• W4311914314 creator A5058076163 @default.
• W4311914314 creator A5060607144 @default.
• W4311914314 creator A5074875737 @default.
• W4311914314 creator A5077202444 @default.
• W4311914314 creator A5079332407 @default.
• W4311914314 creator A5082491541 @default.
• W4311914314 date "2022-12-19" @default.
• W4311914314 modified "2023-10-18" @default.
• W4311914314 title "A novel Oprm1-Cre mouse maintains endogenous expression, function and enables detailed molecular characterization of μ-opioid receptor cells" @default.
• W4311914314 cites W1523806406 @default.
• W4311914314 cites W1591789321 @default.
• W4311914314 cites W1887625685 @default.
• W4311914314 cites W1935339094 @default.
• W4311914314 cites W1977007461 @default.
• W4311914314 cites W1991173233 @default.
• W4311914314 cites W1991992618 @default.
• W4311914314 cites W1993981485 @default.
• W4311914314 cites W2003737145 @default.
• W4311914314 cites W2008147758 @default.
• W4311914314 cites W2011645297 @default.
• W4311914314 cites W2013956365 @default.
• W4311914314 cites W2017860510 @default.
• W4311914314 cites W2022641824 @default.
• W4311914314 cites W2029043236 @default.
• W4311914314 cites W2037917849 @default.
• W4311914314 cites W2050021971 @default.
• W4311914314 cites W2066308284 @default.
• W4311914314 cites W2072602203 @default.
• W4311914314 cites W2084757489 @default.
• W4311914314 cites W2086139880 @default.
• W4311914314 cites W2086644826 @default.
• W4311914314 cites W2089869714 @default.
• W4311914314 cites W2093971225 @default.
• W4311914314 cites W2104569716 @default.
• W4311914314 cites W2119353518 @default.
• W4311914314 cites W2123881866 @default.
• W4311914314 cites W2133224446 @default.
• W4311914314 cites W2296943649 @default.
• W4311914314 cites W2312055187 @default.
• W4311914314 cites W2550769700 @default.
• W4311914314 cites W2566249940 @default.
• W4311914314 cites W2574695133 @default.
• W4311914314 cites W2587845606 @default.
• W4311914314 cites W2806989085 @default.
• W4311914314 cites W2917951125 @default.
• W4311914314 cites W2954931807 @default.
• W4311914314 cites W2981823230 @default.
• W4311914314 cites W2996858474 @default.
• W4311914314 cites W3015101581 @default.
• W4311914314 cites W3019436661 @default.
• W4311914314 cites W3023319216 @default.
• W4311914314 cites W3044538836 @default.
• W4311914314 cites W2069788975 @default.
• W4311914314 doi "https://doi.org/10.1371/journal.pone.0270317" @default.
• W4311914314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36534642" @default.
• W4311914314 hasPublicationYear "2022" @default.
• W4311914314 type Work @default.
• W4311914314 citedByCount "4" @default.
• W4311914314 countsByYear W43119143142023 @default.
• W4311914314 crossrefType "journal-article" @default.
• W4311914314 hasAuthorship W4311914314A5012195642 @default.
• W4311914314 hasAuthorship W4311914314A5013450521 @default.
• W4311914314 hasAuthorship W4311914314A5017805012 @default.
• W4311914314 hasAuthorship W4311914314A5018638118 @default.
• W4311914314 hasAuthorship W4311914314A5022907334 @default.
• W4311914314 hasAuthorship W4311914314A5046541978 @default.
• W4311914314 hasAuthorship W4311914314A5058076163 @default.
• W4311914314 hasAuthorship W4311914314A5060607144 @default.
• W4311914314 hasAuthorship W4311914314A5074875737 @default.
• W4311914314 hasAuthorship W4311914314A5077202444 @default.
• W4311914314 hasAuthorship W4311914314A5079332407 @default.
• W4311914314 hasAuthorship W4311914314A5082491541 @default.
• W4311914314 hasBestOaLocation W43119143141 @default.
• W4311914314 hasConcept C14397066 @default.
• W4311914314 hasConcept C170493617 @default.
• W4311914314 hasConcept C2776029756 @default.
• W4311914314 hasConcept C2776401185 @default.
• W4311914314 hasConcept C2779886867 @default.
• W4311914314 hasConcept C2781063702 @default.
• W4311914314 hasConcept C54355233 @default.
• W4311914314 hasConcept C86803240 @default.
• W4311914314 hasConcept C95444343 @default.
• W4311914314 hasConcept C98274493 @default.
• W4311914314 hasConceptScore W4311914314C14397066 @default.
• W4311914314 hasConceptScore W4311914314C170493617 @default.
• W4311914314 hasConceptScore W4311914314C2776029756 @default.
• W4311914314 hasConceptScore W4311914314C2776401185 @default.
• W4311914314 hasConceptScore W4311914314C2779886867 @default.
• W4311914314 hasConceptScore W4311914314C2781063702 @default.
• W4311914314 hasConceptScore W4311914314C54355233 @default.

• next