FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312003670> ?p ?o ?g. }

• W4312003670 endingPage "e0276115" @default.
• W4312003670 startingPage "e0276115" @default.
• W4312003670 abstract "Human-based organ models can provide strong predictive value to investigate the tropism, virulence, and replication kinetics of viral pathogens. Currently, such models have received widespread attention in the study of SARS-CoV-2 causing the COVID-19 pandemic. Applicable to a large set of organoid models and viruses, we provide a step-by-step work instruction for the infection of human alveolar-like organoids with SARS-CoV-2 in this protocol collection. We also prepared a detailed description on state-of-the-art methodologies to assess the infection impact and the analysis of relevant host factors in organoids. This protocol collection consists of five different sets of protocols. Set 1 describes the protein extraction from human alveolar-like organoids and the determination of protein expression of angiotensin-converting enzyme 2 (ACE2), transmembrane serine protease 2 (TMPRSS2) and FURIN as exemplary host factors of SARS-CoV-2. Set 2 provides detailed guidance on the extraction of RNA from human alveolar-like organoids and the subsequent qPCR to quantify the expression level of ACE2 , TMPRSS2 , and FURIN as host factors of SARS-CoV-2 on the mRNA level. Protocol set 3 contains an in-depth explanation on how to infect human alveolar-like organoids with SARS-CoV-2 and how to quantify the viral replication by plaque assay and viral E gene-based RT-qPCR. Set 4 provides a step-by-step protocol for the isolation of single cells from infected human alveolar-like organoids for further processing in single-cell RNA sequencing or flow cytometry. Set 5 presents a detailed protocol on how to perform the fixation of human alveolar-like organoids and guides through all steps of immunohistochemistry and in situ hybridization to visualize SARS-CoV-2 and its host factors. The infection and all subsequent analytical methods have been successfully validated by biological replications with human alveolar-like organoids based on material from different donors." @default.
• W4312003670 created "2023-01-03" @default.
• W4312003670 creator A5011653179 @default.
• W4312003670 creator A5013745385 @default.
• W4312003670 creator A5017328820 @default.
• W4312003670 creator A5021026009 @default.
• W4312003670 creator A5022789402 @default.
• W4312003670 creator A5031474191 @default.
• W4312003670 creator A5036304585 @default.
• W4312003670 creator A5037226865 @default.
• W4312003670 creator A5040431545 @default.
• W4312003670 creator A5041273179 @default.
• W4312003670 creator A5044748952 @default.
• W4312003670 creator A5046813718 @default.
• W4312003670 creator A5047445643 @default.
• W4312003670 creator A5049409453 @default.
• W4312003670 creator A5052512832 @default.
• W4312003670 creator A5054661753 @default.
• W4312003670 creator A5056510315 @default.
• W4312003670 creator A5060471961 @default.
• W4312003670 creator A5090256680 @default.
• W4312003670 date "2022-12-20" @default.
• W4312003670 modified "2023-10-18" @default.
• W4312003670 title "State-of-the-art analytical methods of viral infections in human lung organoids" @default.
• W4312003670 cites W2137099857 @default.
• W4312003670 cites W2441337908 @default.
• W4312003670 cites W2564455178 @default.
• W4312003670 cites W2744377069 @default.
• W4312003670 cites W2896460201 @default.
• W4312003670 cites W2944466104 @default.
• W4312003670 cites W3001195213 @default.
• W4312003670 cites W3009912996 @default.
• W4312003670 cites W3094609450 @default.
• W4312003670 cites W3101697142 @default.
• W4312003670 cites W3136469856 @default.
• W4312003670 cites W3182324497 @default.
• W4312003670 cites W3195660460 @default.
• W4312003670 cites W3206497217 @default.
• W4312003670 cites W3208619586 @default.
• W4312003670 cites W4205091890 @default.
• W4312003670 cites W4205959140 @default.
• W4312003670 cites W4206967951 @default.
• W4312003670 cites W4283269433 @default.
• W4312003670 cites W4287377969 @default.
• W4312003670 cites W4293240464 @default.
• W4312003670 doi "https://doi.org/10.1371/journal.pone.0276115" @default.
• W4312003670 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36538516" @default.
• W4312003670 hasPublicationYear "2022" @default.
• W4312003670 type Work @default.
• W4312003670 citedByCount "0" @default.
• W4312003670 crossrefType "journal-article" @default.
• W4312003670 hasAuthorship W4312003670A5011653179 @default.
• W4312003670 hasAuthorship W4312003670A5013745385 @default.
• W4312003670 hasAuthorship W4312003670A5017328820 @default.
• W4312003670 hasAuthorship W4312003670A5021026009 @default.
• W4312003670 hasAuthorship W4312003670A5022789402 @default.
• W4312003670 hasAuthorship W4312003670A5031474191 @default.
• W4312003670 hasAuthorship W4312003670A5036304585 @default.
• W4312003670 hasAuthorship W4312003670A5037226865 @default.
• W4312003670 hasAuthorship W4312003670A5040431545 @default.
• W4312003670 hasAuthorship W4312003670A5041273179 @default.
• W4312003670 hasAuthorship W4312003670A5044748952 @default.
• W4312003670 hasAuthorship W4312003670A5046813718 @default.
• W4312003670 hasAuthorship W4312003670A5047445643 @default.
• W4312003670 hasAuthorship W4312003670A5049409453 @default.
• W4312003670 hasAuthorship W4312003670A5052512832 @default.
• W4312003670 hasAuthorship W4312003670A5054661753 @default.
• W4312003670 hasAuthorship W4312003670A5056510315 @default.
• W4312003670 hasAuthorship W4312003670A5060471961 @default.
• W4312003670 hasAuthorship W4312003670A5090256680 @default.
• W4312003670 hasBestOaLocation W43120036701 @default.
• W4312003670 hasConcept C104317684 @default.
• W4312003670 hasConcept C112190416 @default.
• W4312003670 hasConcept C140704245 @default.
• W4312003670 hasConcept C14500911 @default.
• W4312003670 hasConcept C159047783 @default.
• W4312003670 hasConcept C181199279 @default.
• W4312003670 hasConcept C2522874641 @default.
• W4312003670 hasConcept C2776408679 @default.
• W4312003670 hasConcept C2777265534 @default.
• W4312003670 hasConcept C31695470 @default.
• W4312003670 hasConcept C54355233 @default.
• W4312003670 hasConcept C55493867 @default.
• W4312003670 hasConcept C67705224 @default.
• W4312003670 hasConcept C70721500 @default.
• W4312003670 hasConcept C86803240 @default.
• W4312003670 hasConcept C95444343 @default.
• W4312003670 hasConceptScore W4312003670C104317684 @default.
• W4312003670 hasConceptScore W4312003670C112190416 @default.
• W4312003670 hasConceptScore W4312003670C140704245 @default.
• W4312003670 hasConceptScore W4312003670C14500911 @default.
• W4312003670 hasConceptScore W4312003670C159047783 @default.
• W4312003670 hasConceptScore W4312003670C181199279 @default.
• W4312003670 hasConceptScore W4312003670C2522874641 @default.
• W4312003670 hasConceptScore W4312003670C2776408679 @default.
• W4312003670 hasConceptScore W4312003670C2777265534 @default.
• W4312003670 hasConceptScore W4312003670C31695470 @default.
• W4312003670 hasConceptScore W4312003670C54355233 @default.

• next