Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312055819> ?p ?o ?g. }
- W4312055819 endingPage "e12433" @default.
- W4312055819 startingPage "e12433" @default.
- W4312055819 abstract "The clinical need for bone regenerative solutions is expanding with increasing life expectancy and escalating incidence of accidents. Several strategies are being investigated to enhance the osteogenic differentiation of stem cells. We previously reported two different approaches for this purpose, in monolayer and three-dimensional cell culture. The first approach was based on pretreating cells with 5-Aza-dC, a DNA methylation inhibitor, before the applying the differentiation media. The second approach was based on culturing cells on a glass surface during differentiation. In this study, we investigated the potential effect of combining both methods. Our results suggested that both approaches were associated with decreasing global DNA methylation levels. Cells cultured as a monolayer on glass surface showed enhancement in alkaline phosphatase activity at day 10, while 5-Aza-dC pretreatment enhanced the activity at day 5, irrespective of the culture surface. In three-dimensional pellet culture, 5-Aza-dC pretreatment enhanced osteogenesis through Runx-2 and TGF-β1 upregulation while the glass surface induced Osterix. Furthermore, pellets cultured on glass showed upregulation of a group of miRNAs, including pro-osteogenesis miR- 20a and miR -148b and anti-osteogenesis miR -125b, miR -31, miR -138, and miR -133a. Interestingly, 5-Aza-dC was not associated with a change of miRNAs in cells cultured on tissue culture plastic but reverted the upregulated miRNAs on the glass to the basal level. This study confirms the two approaches for enhancing osteogenic differentiation and contradicts their combination." @default.
- W4312055819 created "2023-01-04" @default.
- W4312055819 creator A5017711331 @default.
- W4312055819 creator A5020666989 @default.
- W4312055819 creator A5037314995 @default.
- W4312055819 creator A5051380644 @default.
- W4312055819 creator A5067987540 @default.
- W4312055819 date "2022-12-01" @default.
- W4312055819 modified "2023-10-01" @default.
- W4312055819 title "Non-additive effect of the DNA methylation inhibitor, 5-Aza-dC, and glass as a culture surface on osteogenic differentiation" @default.
- W4312055819 cites W126207357 @default.
- W4312055819 cites W1518081123 @default.
- W4312055819 cites W1533546153 @default.
- W4312055819 cites W1565642631 @default.
- W4312055819 cites W1849400986 @default.
- W4312055819 cites W1967841068 @default.
- W4312055819 cites W1969143454 @default.
- W4312055819 cites W1969668903 @default.
- W4312055819 cites W1969720359 @default.
- W4312055819 cites W1976535297 @default.
- W4312055819 cites W1976571762 @default.
- W4312055819 cites W1987113787 @default.
- W4312055819 cites W1988981144 @default.
- W4312055819 cites W1991214829 @default.
- W4312055819 cites W1991471647 @default.
- W4312055819 cites W1993579134 @default.
- W4312055819 cites W2004152316 @default.
- W4312055819 cites W2010254035 @default.
- W4312055819 cites W2017461886 @default.
- W4312055819 cites W2019981271 @default.
- W4312055819 cites W2028011772 @default.
- W4312055819 cites W2030120875 @default.
- W4312055819 cites W2036814359 @default.
- W4312055819 cites W2038607522 @default.
- W4312055819 cites W2042349555 @default.
- W4312055819 cites W2045217393 @default.
- W4312055819 cites W2050200774 @default.
- W4312055819 cites W206573514 @default.
- W4312055819 cites W2068627477 @default.
- W4312055819 cites W2071758034 @default.
- W4312055819 cites W2081298910 @default.
- W4312055819 cites W2107277218 @default.
- W4312055819 cites W2117809535 @default.
- W4312055819 cites W2133449428 @default.
- W4312055819 cites W2137875149 @default.
- W4312055819 cites W2138889138 @default.
- W4312055819 cites W2148340578 @default.
- W4312055819 cites W2158668089 @default.
- W4312055819 cites W2158883564 @default.
- W4312055819 cites W2162088346 @default.
- W4312055819 cites W2163284854 @default.
- W4312055819 cites W2163946434 @default.
- W4312055819 cites W2166075836 @default.
- W4312055819 cites W2167192576 @default.
- W4312055819 cites W2168222359 @default.
- W4312055819 cites W2206609042 @default.
- W4312055819 cites W2272320188 @default.
- W4312055819 cites W2403880140 @default.
- W4312055819 cites W2404123824 @default.
- W4312055819 cites W2476771769 @default.
- W4312055819 cites W2561525464 @default.
- W4312055819 cites W2606069947 @default.
- W4312055819 cites W2744329737 @default.
- W4312055819 cites W2799579979 @default.
- W4312055819 cites W2894875814 @default.
- W4312055819 cites W2901838922 @default.
- W4312055819 cites W2912137189 @default.
- W4312055819 cites W2919505481 @default.
- W4312055819 cites W2940990046 @default.
- W4312055819 cites W2985987438 @default.
- W4312055819 cites W2991615060 @default.
- W4312055819 cites W2995914317 @default.
- W4312055819 cites W2998171063 @default.
- W4312055819 cites W3022100157 @default.
- W4312055819 cites W3049363087 @default.
- W4312055819 cites W3139247605 @default.
- W4312055819 cites W3195424936 @default.
- W4312055819 cites W3207826790 @default.
- W4312055819 cites W4210519028 @default.
- W4312055819 cites W4213062759 @default.
- W4312055819 cites W51108964 @default.
- W4312055819 cites W1964184380 @default.
- W4312055819 doi "https://doi.org/10.1016/j.heliyon.2022.e12433" @default.
- W4312055819 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36590514" @default.
- W4312055819 hasPublicationYear "2022" @default.
- W4312055819 type Work @default.
- W4312055819 citedByCount "1" @default.
- W4312055819 countsByYear W43120558192023 @default.
- W4312055819 crossrefType "journal-article" @default.
- W4312055819 hasAuthorship W4312055819A5017711331 @default.
- W4312055819 hasAuthorship W4312055819A5020666989 @default.
- W4312055819 hasAuthorship W4312055819A5037314995 @default.
- W4312055819 hasAuthorship W4312055819A5051380644 @default.
- W4312055819 hasAuthorship W4312055819A5067987540 @default.
- W4312055819 hasBestOaLocation W43120558192 @default.
- W4312055819 hasConcept C104317684 @default.
- W4312055819 hasConcept C127561419 @default.
- W4312055819 hasConcept C145059251 @default.