Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312067595> ?p ?o ?g. }
- W4312067595 abstract "ABSTRACT The budding and fission yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe have served as invaluable model organisms to study conserved fundamental cellular processes. Although super-resolution microscopy has in recent years paved the way to a better understanding of the spatial organization of molecules in cells, its wide use in yeasts has remained limited due to the specific know-how and instrumentation required, contrasted with the relative ease of endogenous tagging and live-cell fluorescence microscopy. To facilitate super-resolution microscopy in yeasts, we have extended the ultrastructure expansion microscopy (U-ExM) method to both S. cerevisiae and S. pombe, enabling a 4-fold isotropic expansion. We demonstrate that U-ExM allows imaging of the microtubule cytoskeleton and its associated spindle pole body, notably unveiling the Sfi1p–Cdc31p spatial organization on the appendage bridge structure. In S. pombe, we validate the method by monitoring the homeostatic regulation of nuclear pore complex number through the cell cycle. Combined with NHS-ester pan-labelling, which provides a global cellular context, U-ExM reveals the subcellular organization of these two yeast models and provides a powerful new method to augment the already extensive yeast toolbox. This article has an associated First Person interview with Kerstin Hinterndorfer and Felix Mikus, two of the joint first authors of the paper." @default.
- W4312067595 created "2023-01-04" @default.
- W4312067595 creator A5007002785 @default.
- W4312067595 creator A5019103151 @default.
- W4312067595 creator A5022759895 @default.
- W4312067595 creator A5027464896 @default.
- W4312067595 creator A5030507676 @default.
- W4312067595 creator A5037403931 @default.
- W4312067595 creator A5043956923 @default.
- W4312067595 creator A5050159969 @default.
- W4312067595 creator A5055634778 @default.
- W4312067595 creator A5089063142 @default.
- W4312067595 date "2022-12-15" @default.
- W4312067595 modified "2023-10-10" @default.
- W4312067595 title "Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast" @default.
- W4312067595 cites W1606232948 @default.
- W4312067595 cites W1749612483 @default.
- W4312067595 cites W1966072209 @default.
- W4312067595 cites W1975001517 @default.
- W4312067595 cites W1985646440 @default.
- W4312067595 cites W1990265320 @default.
- W4312067595 cites W1992893959 @default.
- W4312067595 cites W2052947711 @default.
- W4312067595 cites W2070963888 @default.
- W4312067595 cites W2084306261 @default.
- W4312067595 cites W2097379399 @default.
- W4312067595 cites W2119662898 @default.
- W4312067595 cites W2124733101 @default.
- W4312067595 cites W2132613776 @default.
- W4312067595 cites W2134805280 @default.
- W4312067595 cites W2138375072 @default.
- W4312067595 cites W2142393767 @default.
- W4312067595 cites W2157329106 @default.
- W4312067595 cites W2165822900 @default.
- W4312067595 cites W2167837269 @default.
- W4312067595 cites W2186275365 @default.
- W4312067595 cites W2189710370 @default.
- W4312067595 cites W2205715024 @default.
- W4312067595 cites W2291231649 @default.
- W4312067595 cites W2406725268 @default.
- W4312067595 cites W2494923364 @default.
- W4312067595 cites W2626880858 @default.
- W4312067595 cites W2763844770 @default.
- W4312067595 cites W2764331857 @default.
- W4312067595 cites W2775523317 @default.
- W4312067595 cites W2799446434 @default.
- W4312067595 cites W2801425093 @default.
- W4312067595 cites W2876005425 @default.
- W4312067595 cites W2902929870 @default.
- W4312067595 cites W2935467328 @default.
- W4312067595 cites W2951433262 @default.
- W4312067595 cites W2974573793 @default.
- W4312067595 cites W2980582603 @default.
- W4312067595 cites W3014773913 @default.
- W4312067595 cites W3021302753 @default.
- W4312067595 cites W3021567235 @default.
- W4312067595 cites W3029927751 @default.
- W4312067595 cites W3038485847 @default.
- W4312067595 cites W3040121687 @default.
- W4312067595 cites W3046519579 @default.
- W4312067595 cites W3080282607 @default.
- W4312067595 cites W3080888834 @default.
- W4312067595 cites W3128903828 @default.
- W4312067595 cites W3133448715 @default.
- W4312067595 cites W3134533921 @default.
- W4312067595 cites W3147908094 @default.
- W4312067595 cites W3150991717 @default.
- W4312067595 cites W3159354973 @default.
- W4312067595 cites W3176144469 @default.
- W4312067595 cites W3204118351 @default.
- W4312067595 cites W3212208195 @default.
- W4312067595 cites W4206382856 @default.
- W4312067595 cites W4220669079 @default.
- W4312067595 cites W4226008255 @default.
- W4312067595 cites W4296788965 @default.
- W4312067595 cites W2888238888 @default.
- W4312067595 doi "https://doi.org/10.1242/jcs.260240" @default.
- W4312067595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36524422" @default.
- W4312067595 hasPublicationYear "2022" @default.
- W4312067595 type Work @default.
- W4312067595 citedByCount "6" @default.
- W4312067595 countsByYear W43120675952022 @default.
- W4312067595 countsByYear W43120675952023 @default.
- W4312067595 crossrefType "journal-article" @default.
- W4312067595 hasAuthorship W4312067595A5007002785 @default.
- W4312067595 hasAuthorship W4312067595A5019103151 @default.
- W4312067595 hasAuthorship W4312067595A5022759895 @default.
- W4312067595 hasAuthorship W4312067595A5027464896 @default.
- W4312067595 hasAuthorship W4312067595A5030507676 @default.
- W4312067595 hasAuthorship W4312067595A5037403931 @default.
- W4312067595 hasAuthorship W4312067595A5043956923 @default.
- W4312067595 hasAuthorship W4312067595A5050159969 @default.
- W4312067595 hasAuthorship W4312067595A5055634778 @default.
- W4312067595 hasAuthorship W4312067595A5089063142 @default.
- W4312067595 hasBestOaLocation W43120675951 @default.
- W4312067595 hasConcept C105702510 @default.
- W4312067595 hasConcept C121332964 @default.
- W4312067595 hasConcept C142669718 @default.
- W4312067595 hasConcept C1491633281 @default.
- W4312067595 hasConcept C151730666 @default.