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- W4312077991 abstract "Abstract A growing number of studies have investigated the feasibility of utilizing hemp by-products as livestock feedstuffs; however, their impact on livestock microbiomes remains unexplored. Here, we evaluated the effects of feeding hempseed cake on the gastrointestinal, respiratory, and reproductive microbiota in beef heifers. Angus-crossbred heifers (19-months old) were fed a corn-based finishing diet containing 20% hempseed cake as a substitute for 20% corn dried distillers’ grains with solubles (DM basis; Control; n = 16/group) for 111 days until slaughter. Ruminal fluid and deep nasopharyngeal swabs (days 0, 7, 42, 70 and 98), and vaginal and uterine swabs (at slaughter) were collected, and the microbiota assessed using 16S rRNA gene sequencing. Diet affected the community structure of the ruminal (d7– 98; 0.06 ≤ R 2 ≤ 0.12; P < 0.05), nasopharyngeal (d 98; R 2 = 0.18; P < 0.001), and vaginal (R 2 = 0.06; P < 0.01) microbiota. Heifers fed hempseed cake had increased microbial diversity in the rumen, reduced microbial richness in the vagina, and greater microbial diversity and richness in the uterus. In addition to the distinct microbial communities in the rumen, nasopharynx, vagina and uterus, we identified 28 core taxa that were shared (≥ 60% of all samples) across these sampling locations. Feeding hempseed cake appeared to alter the bovine gut, respiratory and reproductive microbiota. Our findings also highlight the need for research evaluating the impact of hemp-associated food and personal care products on the human microbiome." @default.
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- W4312077991 date "2022-12-21" @default.
- W4312077991 modified "2023-10-18" @default.
- W4312077991 title "Feeding hempseed cake alters the bovine gut, respiratory and reproductive microbiota" @default.
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- W4312077991 doi "https://doi.org/10.21203/rs.3.rs-2199190/v2" @default.
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