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- W4312093048 abstract "S-Adenosylmethionine (SAM) is a crucial small-molecule metabolite widely used in food and medicine. The development of high-throughput biosensors for SAM biosynthesis can significantly improve the titer of SAM. This paper constructed a synthetic transcription factor (TF)-based biosensor for SAM detecting in Saccharomyces cerevisiae. The synthetic TF, named MetJ-hER-VP16, consists of an Escherichia coli-derived DNA-binding domain MetJ, GS linker, the human estrogen receptor binding domain hER, and the viral activation domain VP16. The synthetic biosensor is capable of sensing SAM in a dose-dependent manner with fluorescence as the output. Additionally, it is tightly regulated by the inducer SAM and β-estradiol, which means that the fluorescence output is only available when both are present together. The synthetic SAM biosensor could potentially be applied for high-throughput metabolic engineering and is expected to improve SAM production." @default.
- W4312093048 created "2023-01-04" @default.
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- W4312093048 date "2022-12-22" @default.
- W4312093048 modified "2023-10-14" @default.
- W4312093048 title "Development of a synthetic transcription factor-based S-adenosylmethionine biosensor in Saccharomyces cerevisiae" @default.
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- W4312093048 doi "https://doi.org/10.1007/s10529-022-03338-8" @default.
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