FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312114562> ?p ?o ?g. }

• W4312114562 endingPage "1281" @default.
• W4312114562 startingPage "1267" @default.
• W4312114562 abstract "Insect cuticle is an apical extracellular matrix produced by the epidermis, tracheal, hind- and foregut epithelia during embryogenesis and renewed during molting and metamorphosis. However, the underlying regulatory mechanism for embryonic cuticle formation remains largely unclear. Here, we investigate the function of the transcription factor POUM2 in the embryonic cuticular formation in Bombyx mori, a model lepidopteran insect. Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein-9-mediated knockout of POUM2 resulted in the defect of cuticular deposition, pigmentation, and sclerotization in the embryos. Differentially expressed transcripts analysis of 7-d-old embryos identified 174 up- or downregulated cuticular protein transcripts, 8 upregulated chitin degradation transcripts, 2 downregulated chitin synthesis transcripts and 48 up- or downregulated transcription factor transcripts in the POUM2-/- embryos. The expression levels of the key factors of the tyrosine metabolic pathway, such as tyrosine hydroxylase (Th), Dopa decarboxylase (DDC), and arylalkylamine N-acetyltransferase (aaNAT), were significantly decreased in the POUM2-/- embryos. POUM2 isoform POUM2-L specifically bound the POU cis-regulatory element (CRE) in the Th promoter and increased the transcription of Th, whereas POUM2-S could not bind the POU CRE, although it also increased the transcription of Th. Heterogeneous nuclear ribonucleoprotein Squid-1 directly bound the POUM2 pre-mRNA (messenger RNA) and inhibited the alternative splicing of POUM2-L to POUM2-S mRNA. These results suggest that POUM2 participates in the cuticular formation by regulating the chitin and cuticular protein synthesis and metabolism, and the cuticular pigmentation and sclerotization by regulating tyrosine metabolism during embryogenesis. This study provides new insights into novel function of POUM2 in embryogenesis." @default.
• W4312114562 created "2023-01-04" @default.
• W4312114562 creator A5010252106 @default.
• W4312114562 creator A5027692459 @default.
• W4312114562 creator A5049450034 @default.
• W4312114562 creator A5051284726 @default.
• W4312114562 creator A5071258270 @default.
• W4312114562 creator A5071873598 @default.
• W4312114562 creator A5085969939 @default.
• W4312114562 creator A5089803646 @default.
• W4312114562 date "2023-02-08" @default.
• W4312114562 modified "2023-10-17" @default.
• W4312114562 title "Alternative splicing of <i>POUM2</i> regulates embryonic cuticular formation and tanning in <i>Bombyx mori</i>" @default.
• W4312114562 cites W1110059699 @default.
• W4312114562 cites W119625346 @default.
• W4312114562 cites W1198705353 @default.
• W4312114562 cites W1505887342 @default.
• W4312114562 cites W1595185468 @default.
• W4312114562 cites W160840648 @default.
• W4312114562 cites W1967608632 @default.
• W4312114562 cites W1969148951 @default.
• W4312114562 cites W1986953436 @default.
• W4312114562 cites W1997046409 @default.
• W4312114562 cites W2002921297 @default.
• W4312114562 cites W2004689349 @default.
• W4312114562 cites W2007480067 @default.
• W4312114562 cites W2008861638 @default.
• W4312114562 cites W2011653571 @default.
• W4312114562 cites W2012870719 @default.
• W4312114562 cites W2013085811 @default.
• W4312114562 cites W2023035275 @default.
• W4312114562 cites W2027102545 @default.
• W4312114562 cites W2030237639 @default.
• W4312114562 cites W2066034814 @default.
• W4312114562 cites W2080442200 @default.
• W4312114562 cites W2081534563 @default.
• W4312114562 cites W2084083756 @default.
• W4312114562 cites W2096131346 @default.
• W4312114562 cites W2097065948 @default.
• W4312114562 cites W2098832713 @default.
• W4312114562 cites W2103823160 @default.
• W4312114562 cites W2108053190 @default.
• W4312114562 cites W2119670468 @default.
• W4312114562 cites W2127689082 @default.
• W4312114562 cites W2128641591 @default.
• W4312114562 cites W2139159095 @default.
• W4312114562 cites W2141458291 @default.
• W4312114562 cites W2150916276 @default.
• W4312114562 cites W2153833484 @default.
• W4312114562 cites W2155897862 @default.
• W4312114562 cites W2166526976 @default.
• W4312114562 cites W2167267182 @default.
• W4312114562 cites W2169062325 @default.
• W4312114562 cites W2187893662 @default.
• W4312114562 cites W2321544564 @default.
• W4312114562 cites W2349289192 @default.
• W4312114562 cites W2563692976 @default.
• W4312114562 cites W2624830532 @default.
• W4312114562 cites W2742054541 @default.
• W4312114562 cites W2767642029 @default.
• W4312114562 cites W2994299417 @default.
• W4312114562 cites W2997634099 @default.
• W4312114562 cites W3015954223 @default.
• W4312114562 cites W3021194431 @default.
• W4312114562 cites W3027512590 @default.
• W4312114562 cites W3190513588 @default.
• W4312114562 doi "https://doi.org/10.1111/1744-7917.13164" @default.
• W4312114562 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36562105" @default.
• W4312114562 hasPublicationYear "2023" @default.
• W4312114562 type Work @default.
• W4312114562 citedByCount "1" @default.
• W4312114562 countsByYear W43121145622022 @default.
• W4312114562 crossrefType "journal-article" @default.
• W4312114562 hasAuthorship W4312114562A5010252106 @default.
• W4312114562 hasAuthorship W4312114562A5027692459 @default.
• W4312114562 hasAuthorship W4312114562A5049450034 @default.
• W4312114562 hasAuthorship W4312114562A5051284726 @default.
• W4312114562 hasAuthorship W4312114562A5071258270 @default.
• W4312114562 hasAuthorship W4312114562A5071873598 @default.
• W4312114562 hasAuthorship W4312114562A5085969939 @default.
• W4312114562 hasAuthorship W4312114562A5089803646 @default.
• W4312114562 hasConcept C104317684 @default.
• W4312114562 hasConcept C153911025 @default.
• W4312114562 hasConcept C2778487026 @default.
• W4312114562 hasConcept C55493867 @default.
• W4312114562 hasConcept C86339819 @default.
• W4312114562 hasConcept C86803240 @default.
• W4312114562 hasConcept C95444343 @default.
• W4312114562 hasConceptScore W4312114562C104317684 @default.
• W4312114562 hasConceptScore W4312114562C153911025 @default.
• W4312114562 hasConceptScore W4312114562C2778487026 @default.
• W4312114562 hasConceptScore W4312114562C55493867 @default.
• W4312114562 hasConceptScore W4312114562C86339819 @default.
• W4312114562 hasConceptScore W4312114562C86803240 @default.
• W4312114562 hasConceptScore W4312114562C95444343 @default.
• W4312114562 hasFunder F4320321001 @default.
• W4312114562 hasIssue "5" @default.
• W4312114562 hasLocation W43121145621 @default.

• next