Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312114912> ?p ?o ?g. }
- W4312114912 abstract "Ribbon synapses of cochlear inner hair cells (IHCs) are specialized to indefatigably transmit sound information at high rates. To understand the underlying mechanisms, structure-function analysis of the active zone (AZ) of these synapses is essential. Previous electron microscopy studies of synaptic vesicle (SV) dynamics at the IHC AZ used potassium stimulation, which limited the temporal resolution to minutes. Here, we established optogenetic IHC stimulation followed by quick freezing within milliseconds and electron tomography to study the ultrastructure of functional synapse states with good temporal resolution in mice. We characterized optogenetic IHC stimulation by patch-clamp recordings from IHCs and postsynaptic boutons revealing robust IHC depolarization and neurotransmitter release. Ultrastructurally, the number of docked SVs increased upon short (17–25 ms) and long (48–76 ms) light stimulation paradigms. We did not observe enlarged SVs or other morphological correlates of homotypic fusion events. Our results indicate a rapid recruitment of SVs to the docked state upon stimulation and suggest that univesicular release prevails as the quantal mechanism of exocytosis at IHC ribbon synapses." @default.
- W4312114912 created "2023-01-04" @default.
- W4312114912 creator A5014847511 @default.
- W4312114912 creator A5021834441 @default.
- W4312114912 creator A5027950398 @default.
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- W4312114912 creator A5071154621 @default.
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- W4312114912 creator A5085199270 @default.
- W4312114912 creator A5089703846 @default.
- W4312114912 creator A5091020916 @default.
- W4312114912 creator A5091197463 @default.
- W4312114912 date "2022-12-23" @default.
- W4312114912 modified "2023-10-10" @default.
- W4312114912 title "Optogenetics and electron tomography for structure-function analysis of cochlear ribbon synapses" @default.
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