Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312139626> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W4312139626 abstract "<h3>Objectives</h3> Patient-derived cancer organoids have quickly developed as valuable tools for drug testing as they better represent the genetic background of the patient cohort. We recently published a protocol for establishing EC organoids from all types and grades of EC (Berg et al, Nat Comms Med 2021). We here present data from a prospectively collected biobank of organoids including all EC molecular subclasses. Models have been extensively profiled and evaluated for drug response, supporting high heterogeneity in EC. <h3>Methods</h3> Organoids were prospectively derived from resected EC tissue from consenting patients and cultured long-term in a chemically defined medium. Orthotopic xenograft mouse models, representing all subtypes of EC, were established by intra-uterine injection of organoids. All organoids were characterized by IHC, WES and RNA sequencing, and by single cell phenotyping. Organoids were treated with carboplatin, paclitaxel, and small molecule inhibitors against PARP, CHEK1/2, PIK3CA/mTOR, and CDK4/6. <h3>Results</h3> The organoids reflect the main molecular EC subtypes, including POLE, MSI, copy-number low and copy-number high models. We identified matched molecular alterations in patient tissue and corresponding organoids, even after long-term culturing and expansion in vivo. Model-specific chemotherapy responses were observed and reproduced in mouse models for selected organoids. Carboplatin-resistant organoids had more alterations in platinum-related genes, including BAX, DAB2IP, ATM and CASP2. However, type of genetic alteration differed between the resistant organoids. Treatment with small-molecule inhibitors identified heterogenous drug responses. <h3>Conclusions</h3> This state-of-the-art preclinical platform provides clinically relevant tools for use in preclinical drug trials." @default.
- W4312139626 created "2023-01-04" @default.
- W4312139626 creator A5000035191 @default.
- W4312139626 creator A5015485203 @default.
- W4312139626 creator A5019754075 @default.
- W4312139626 creator A5035785989 @default.
- W4312139626 creator A5038328067 @default.
- W4312139626 creator A5041980697 @default.
- W4312139626 creator A5045128636 @default.
- W4312139626 creator A5046533177 @default.
- W4312139626 creator A5060184451 @default.
- W4312139626 creator A5077781615 @default.
- W4312139626 creator A5080010131 @default.
- W4312139626 creator A5090908683 @default.
- W4312139626 creator A5091059608 @default.
- W4312139626 date "2022-12-01" @default.
- W4312139626 modified "2023-10-18" @default.
- W4312139626 title "20/#1057 Integrated profiling of a prospective endometrial cancer organoid biobank reveals high heterogeneity" @default.
- W4312139626 doi "https://doi.org/10.1136/ijgc-2022-igcs.64" @default.
- W4312139626 hasPublicationYear "2022" @default.
- W4312139626 type Work @default.
- W4312139626 citedByCount "0" @default.
- W4312139626 crossrefType "proceedings-article" @default.
- W4312139626 hasAuthorship W4312139626A5000035191 @default.
- W4312139626 hasAuthorship W4312139626A5015485203 @default.
- W4312139626 hasAuthorship W4312139626A5019754075 @default.
- W4312139626 hasAuthorship W4312139626A5035785989 @default.
- W4312139626 hasAuthorship W4312139626A5038328067 @default.
- W4312139626 hasAuthorship W4312139626A5041980697 @default.
- W4312139626 hasAuthorship W4312139626A5045128636 @default.
- W4312139626 hasAuthorship W4312139626A5046533177 @default.
- W4312139626 hasAuthorship W4312139626A5060184451 @default.
- W4312139626 hasAuthorship W4312139626A5077781615 @default.
- W4312139626 hasAuthorship W4312139626A5080010131 @default.
- W4312139626 hasAuthorship W4312139626A5090908683 @default.
- W4312139626 hasAuthorship W4312139626A5091059608 @default.
- W4312139626 hasBestOaLocation W43121396261 @default.
- W4312139626 hasConcept C116567970 @default.
- W4312139626 hasConcept C121608353 @default.
- W4312139626 hasConcept C126322002 @default.
- W4312139626 hasConcept C185592680 @default.
- W4312139626 hasConcept C2776694085 @default.
- W4312139626 hasConcept C2777292972 @default.
- W4312139626 hasConcept C2778239845 @default.
- W4312139626 hasConcept C2781451048 @default.
- W4312139626 hasConcept C31695470 @default.
- W4312139626 hasConcept C502942594 @default.
- W4312139626 hasConcept C60644358 @default.
- W4312139626 hasConcept C70721500 @default.
- W4312139626 hasConcept C71924100 @default.
- W4312139626 hasConcept C86803240 @default.
- W4312139626 hasConcept C95444343 @default.
- W4312139626 hasConceptScore W4312139626C116567970 @default.
- W4312139626 hasConceptScore W4312139626C121608353 @default.
- W4312139626 hasConceptScore W4312139626C126322002 @default.
- W4312139626 hasConceptScore W4312139626C185592680 @default.
- W4312139626 hasConceptScore W4312139626C2776694085 @default.
- W4312139626 hasConceptScore W4312139626C2777292972 @default.
- W4312139626 hasConceptScore W4312139626C2778239845 @default.
- W4312139626 hasConceptScore W4312139626C2781451048 @default.
- W4312139626 hasConceptScore W4312139626C31695470 @default.
- W4312139626 hasConceptScore W4312139626C502942594 @default.
- W4312139626 hasConceptScore W4312139626C60644358 @default.
- W4312139626 hasConceptScore W4312139626C70721500 @default.
- W4312139626 hasConceptScore W4312139626C71924100 @default.
- W4312139626 hasConceptScore W4312139626C86803240 @default.
- W4312139626 hasConceptScore W4312139626C95444343 @default.
- W4312139626 hasLocation W43121396261 @default.
- W4312139626 hasOpenAccess W4312139626 @default.
- W4312139626 hasPrimaryLocation W43121396261 @default.
- W4312139626 hasRelatedWork W1569517976 @default.
- W4312139626 hasRelatedWork W2003594160 @default.
- W4312139626 hasRelatedWork W2329492928 @default.
- W4312139626 hasRelatedWork W2350445292 @default.
- W4312139626 hasRelatedWork W2417982295 @default.
- W4312139626 hasRelatedWork W2748952813 @default.
- W4312139626 hasRelatedWork W2896450660 @default.
- W4312139626 hasRelatedWork W2899084033 @default.
- W4312139626 hasRelatedWork W4244439098 @default.
- W4312139626 hasRelatedWork W4311689944 @default.
- W4312139626 isParatext "false" @default.
- W4312139626 isRetracted "false" @default.
- W4312139626 workType "article" @default.