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- W4312139919 endingPage "15801" @default.
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- W4312139919 abstract "Small particles in natural sources are a subject of interest for their potential role in intercellular, inter-organism, and inter-species interactions, but their harvesting and assessment present a challenge due to their small size and transient identity. We applied a recently developed interferometric light microscopy (ILM) to assess the number density and hydrodynamic radius (Rh) of isolated small cellular particles (SCPs) from blood preparations (plasma and washed erythrocytes) (B), spruce needle homogenate (S), suspension of flagellae of microalgae Tetraselmis chuii (T), conditioned culture media of microalgae Phaeodactylum tricornutum (P), and liposomes (L). The aliquots were also assessed by flow cytometry (FCM), dynamic light scattering (DLS), ultraviolet-visible spectrometry (UV-vis), and imaging by cryogenic transmission electron microscopy (cryo-TEM). In Rh, ILM showed agreement with DLS within the measurement error in 10 out of 13 samples and was the only method used here that yielded particle density. Cryo-TEM revealed that representative SCPs from Tetraselmis chuii flagella (T) did not have a globular shape, so the interpretation by Rh of the batch methods was biased. Cryo-TEM showed the presence of thin filaments in isolates from Phaeodactylum tricornutum conditioned culture media (P), which provides an explanation for the considerably larger Rh obtained by batch methods than the sizes of particles observed by cryo-TEM images. ILM proved convenient for assessment of number density and Rh of SCPs in blood preparations (e.g., plasma); therefore, its use in population and clinical studies is indicated." @default.
- W4312139919 created "2023-01-04" @default.
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- W4312139919 date "2022-12-13" @default.
- W4312139919 modified "2023-10-14" @default.
- W4312139919 title "Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy" @default.
- W4312139919 cites W1821396137 @default.
- W4312139919 cites W1839188703 @default.
- W4312139919 cites W1976805367 @default.
- W4312139919 cites W1992226521 @default.
- W4312139919 cites W2007499410 @default.
- W4312139919 cites W2033112925 @default.
- W4312139919 cites W2044275624 @default.
- W4312139919 cites W2062099500 @default.
- W4312139919 cites W2071281057 @default.
- W4312139919 cites W2093635335 @default.
- W4312139919 cites W2103972113 @default.
- W4312139919 cites W2117208227 @default.
- W4312139919 cites W2124242076 @default.
- W4312139919 cites W2125087964 @default.
- W4312139919 cites W2128111546 @default.
- W4312139919 cites W2131083445 @default.
- W4312139919 cites W2131883639 @default.
- W4312139919 cites W2136142923 @default.
- W4312139919 cites W2137246476 @default.
- W4312139919 cites W2142512073 @default.
- W4312139919 cites W2147356401 @default.
- W4312139919 cites W2159212838 @default.
- W4312139919 cites W2170852633 @default.
- W4312139919 cites W2191816501 @default.
- W4312139919 cites W2431662627 @default.
- W4312139919 cites W2464767991 @default.
- W4312139919 cites W2517538436 @default.
- W4312139919 cites W2531698483 @default.
- W4312139919 cites W2550469376 @default.
- W4312139919 cites W2577474847 @default.
- W4312139919 cites W2594551672 @default.
- W4312139919 cites W2787963369 @default.
- W4312139919 cites W2797363227 @default.
- W4312139919 cites W2904555432 @default.
- W4312139919 cites W2938074487 @default.
- W4312139919 cites W2955985180 @default.
- W4312139919 cites W2970895669 @default.
- W4312139919 cites W2972028067 @default.
- W4312139919 cites W2983828425 @default.
- W4312139919 cites W3001058124 @default.
- W4312139919 cites W3014503170 @default.
- W4312139919 cites W3044519293 @default.
- W4312139919 cites W3074954585 @default.
- W4312139919 cites W3097127071 @default.
- W4312139919 cites W3104477275 @default.
- W4312139919 cites W3106817752 @default.
- W4312139919 cites W3109744624 @default.
- W4312139919 cites W3112214030 @default.
- W4312139919 cites W3116681155 @default.
- W4312139919 cites W3159866433 @default.
- W4312139919 cites W3160872344 @default.
- W4312139919 cites W3160930536 @default.
- W4312139919 cites W3176253183 @default.
- W4312139919 cites W3215325225 @default.
- W4312139919 cites W3215926082 @default.
- W4312139919 cites W3216504306 @default.
- W4312139919 cites W4220972537 @default.
- W4312139919 cites W4221105891 @default.
- W4312139919 cites W4224114413 @default.
- W4312139919 cites W4225710739 @default.
- W4312139919 cites W4229454482 @default.
- W4312139919 cites W4244920887 @default.
- W4312139919 cites W4281298594 @default.
- W4312139919 cites W4286005590 @default.
- W4312139919 cites W4289111711 @default.
- W4312139919 cites W4289175125 @default.
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- W4312139919 doi "https://doi.org/10.3390/ijms232415801" @default.
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- W4312139919 hasPublicationYear "2022" @default.
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