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- W4312196348 abstract "Immunophenotyping using high dimensional flow cytometry is a central component of human immune system multi-omic studies. We present four high parameter flow cytometry panels for deep immunophenotyping of human peripheral blood mononuclear cells (PBMC). This set of four 25+ color panels include 64 cell surface markers to resolve broad immune compartment populations, as well as activation and memory of specific T, B, natural killer (NK), and myeloid lineages. Common lineage bridging markers are integrated into each panel to allow for inter-panel quality control through major lineage frequency verification. These panels were developed using a five laser BD Symphony A5 conventional cytometer and successfully transferred to a five laser Cytek Aurora spectral cytometer capable of acquiring the panels. Nine representative PBMC samples were stained with the four phenotyping panels and acquired on both instruments to evaluate population frequency and visual staining patterns for gating between the systems. Both instruments produced comparable high quality flow cytometry data and supported our decision to acquire samples on the spectral cytometer moving forward. This modular set of panels and instrument performance metrics provide guidelines for designing flow cytometry experiments suitable for longitudinal or cross-sectional immune profiling." @default.
- W4312196348 created "2023-01-04" @default.
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- W4312196348 date "2023-01-13" @default.
- W4312196348 modified "2023-10-01" @default.
- W4312196348 title "Cross‐platform immunophenotyping of human peripheral blood mononuclear cells with four high‐dimensional flow cytometry panels" @default.
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- W4312196348 cites W1983812780 @default.
- W4312196348 cites W1984378291 @default.
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- W4312196348 cites W2001775594 @default.
- W4312196348 cites W2027822830 @default.
- W4312196348 cites W2043076395 @default.
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- W4312196348 cites W2087537126 @default.
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- W4312196348 cites W2117443606 @default.
- W4312196348 cites W2131635221 @default.
- W4312196348 cites W2155764740 @default.
- W4312196348 cites W2313662676 @default.
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- W4312196348 doi "https://doi.org/10.1002/cyto.a.24715" @default.
- W4312196348 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36571245" @default.
- W4312196348 hasPublicationYear "2023" @default.
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