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- W4312197056 abstract "ABSTRACT The ubiquitous volume-regulated anion channels (VRACs), which are composed of LRRC8 proteins, facilitate cell volume homeostasis, and contribute to many other physiological processes. Prior studies demonstrated that treatment with non-specific VRAC blockers, or brain-specific deletion of the essential VRAC subunit LRRC8A, are highly protective in rodent stroke. In this work, we tested the widely accepted idea that harmful effects of VRACs in the brain are mediated by pathological release of the excitatory transmitter glutamate. We used two molecular genetic strategies to ablate LRRC8A expression in either brain astrocytes only (inducible deletion of Lrrc8a flox/flox with Aldh1l1 CreERT2 ) or the majority of brain cells (neurons, astrocytes, and oligodendrocytes with Nestin Cre ). To produce stroke, genetically modified mice were subjected to a 40-minute occlusion of the middle cerebral artery. The inducible deletion of astrocytic LRRC8A yielded no histological or behavioral protection. In contrast, the brain-wide LRRC8A knockout reduced ischemic infarction by ~50% in both heterozygotes (Het) and the full Lrrc8a knockout (KO) as compared to the control Lrrc8a flox/+ genotype. However, despite identical brain damage, Het and KO mice dramatically differed in their VRAC activities. Het mice had full swelling-activated glutamate release, while KO animals showed its virtual absence. These new findings refute the notion that VRAC-mediated glutamate release plays significant role in ischemic brain injury." @default.
- W4312197056 created "2023-01-04" @default.
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- W4312197056 date "2022-12-13" @default.
- W4312197056 modified "2023-10-16" @default.
- W4312197056 title "Conditional deletion of LRRC8A in the brain protects against stroke damage independently of effect on swelling-activated glutamate release" @default.
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- W4312197056 doi "https://doi.org/10.1101/2022.12.13.520314" @default.
- W4312197056 hasPublicationYear "2022" @default.
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