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- W4312201137 abstract "Theca cells (TCs) play a unique role in the structure and function of the ovary. They are not only the structural basis of the follicle but also the androgen-secreting cells in female mammals, which can affect the normal development and atresia of the follicle. The results showed that melatonin receptor (MTR) MT1 and MT2 were expressed on sheep TCs. In the present study, the effects of different concentrations of MT at 0, 10-10, 10-8, 10-6 and 10-4 M/L on sheep TCs with regards to the antioxidant levels, proliferation, apoptosis and steroid hormone secretion were investigated. The results showed that in sheep TCs, all concentrations of MT significantly decreased reactive oxygen species (ROS) concentration and BAX expression; increased Cat, Sod1, and BCL-2 expression. The proliferation viability of TCs was significantly inhibited in all groups except for 10-10 M/L MT, and the expression of cyclin D1 and CDK4 was significantly reduced. MT significantly increased StAR expression and progesterone secretion in TCs, but there was no significant effect on androgen secretion and CYP11A1, CYP17A1 and 3β-HSD expression in all groups. MT-induced progesterone secretion was completely inhibited by Luzindole (a nonspecific MT1 and MT2 inhibitor) and partially inhibited by 4p-PDOT (specific MT2 inhibitor). MT-induced progesterone secretion can be inhibited by LY294002 (PI3K/AKT pathway inhibitor). This study indicated that MT inhibits apoptosis and proliferation of in vitro cultured sheep TCs, which has implications for slowing ovarian atresia and aging. MT activates the PI3K/Akt pathway to mediate the synthesis and secretion of progesterone by TCs. This study provides a basis for further exploration of the role of TCs on follicle development and ovarian steroid hormone secretion." @default.
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- W4312201137 date "2023-03-01" @default.
- W4312201137 modified "2023-09-30" @default.
- W4312201137 title "Effects of melatonin on development and hormone secretion of sheep theca cells in vitro" @default.
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- W4312201137 cites W1993511574 @default.
- W4312201137 cites W2005318658 @default.
- W4312201137 cites W2010152967 @default.
- W4312201137 cites W2011689307 @default.
- W4312201137 cites W2012482453 @default.
- W4312201137 cites W2036206595 @default.
- W4312201137 cites W2038371031 @default.
- W4312201137 cites W2042464244 @default.
- W4312201137 cites W2050665022 @default.
- W4312201137 cites W2050845656 @default.
- W4312201137 cites W2050874941 @default.
- W4312201137 cites W2066951391 @default.
- W4312201137 cites W2066976230 @default.
- W4312201137 cites W2067938814 @default.
- W4312201137 cites W2068983016 @default.
- W4312201137 cites W2074002921 @default.
- W4312201137 cites W2076440297 @default.
- W4312201137 cites W2092889645 @default.
- W4312201137 cites W2099230387 @default.
- W4312201137 cites W2113705043 @default.
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- W4312201137 cites W2150394551 @default.
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- W4312201137 cites W2163762391 @default.
- W4312201137 cites W2167550453 @default.
- W4312201137 cites W2169717724 @default.
- W4312201137 cites W2463111790 @default.
- W4312201137 cites W2551312221 @default.
- W4312201137 cites W2566570627 @default.
- W4312201137 cites W2617886496 @default.
- W4312201137 cites W2743873438 @default.
- W4312201137 cites W2761264538 @default.
- W4312201137 cites W2763710570 @default.
- W4312201137 cites W2774741827 @default.
- W4312201137 cites W2777313371 @default.
- W4312201137 cites W2788649212 @default.
- W4312201137 cites W2789314241 @default.
- W4312201137 cites W2804051669 @default.
- W4312201137 cites W2805162056 @default.
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- W4312201137 cites W2897996498 @default.
- W4312201137 cites W2911051876 @default.
- W4312201137 cites W2911348880 @default.
- W4312201137 cites W2912567453 @default.
- W4312201137 cites W2915979633 @default.
- W4312201137 cites W2921282526 @default.
- W4312201137 cites W2937346106 @default.
- W4312201137 cites W2969482599 @default.
- W4312201137 cites W2973011115 @default.
- W4312201137 cites W3005110232 @default.
- W4312201137 cites W3035350468 @default.
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- W4312201137 cites W3042139413 @default.
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- W4312201137 cites W3111792822 @default.
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- W4312201137 cites W3185638958 @default.
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- W4312201137 doi "https://doi.org/10.1016/j.theriogenology.2022.12.036" @default.
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- W4312201137 hasPublicationYear "2023" @default.
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