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- W4312219489 abstract "We described a strategy for the enzymatic synthesis of 1-deoxy and 1,2-deoxyketoses from the aliphatic α-ketoacids, pyruvate and 2-oxobutyrate, as donors and natural aldoses of variable chain length as acceptors, catalyzed by thermostable transketolase variants from Geobacillus stearothermophilus (TKgst). Analytical studies have been carried out on a panel of TKgst variants with the appropriate substrates allowing to select the best combinations and to apply it to the preparative scale synthesis of 1-deoxy and 1,2-deoxyketoses obtained with good to excellent isolated yields (61%–86%). To optimize the strategy, and as a proof of principle, the α-ketoacids pyruvate and 2-oxobutyrate were generated in situ from the corresponding d-amino acids d-alanine and d-homoalanine respectively, using a thermostable d-amino acid oxidase dAAO4536 that was selected from a screening of 55 putative DAAOs provided by Prozomix Limited. Hence, a one-pot one step procedure was performed at 50 °C by coupling dAAO4536 and the best TKgst variant H102L/L118I/H474S in the presence of d-alanine or d-homoalanine as α-ketoacids precursors and d-erythrose as acceptor substrate. The corresponding 1-deoxy and 1,2-dideoxyketoses were isolated with good yields (64% and 72% respectively, out of two steps)." @default.
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- W4312219489 date "2022-12-29" @default.
- W4312219489 modified "2023-10-03" @default.
- W4312219489 title "Synthesis of Deoxysugars from Aliphatic α‐Ketoacids and Aldoses Catalyzed by Thermostable Transketolase Variants from <i>Geobacillus stearothermophilus</i>" @default.
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- W4312219489 doi "https://doi.org/10.1002/adsc.202201190" @default.
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