Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312223721> ?p ?o ?g. }
- W4312223721 endingPage "51" @default.
- W4312223721 startingPage "34" @default.
- W4312223721 abstract "Fish basal epidermal cells, known as keratocytes, are well-suited for cell migration studies. In vitro, isolated keratocytes adopt a stereotyped shape with a large fan-shaped lamellipodium and a nearly spherical cell body. However, in their native in vivo environment, these cells adopt a significantly different shape during their rapid migration toward wounds. Within the epidermis, keratocytes experience two-dimensional (2D) confinement between the outer epidermal cell layer and the basement membrane; these two deformable surfaces constrain keratocyte cell bodies to be flatter in vivo than in isolation. In vivo keratocytes also exhibit a relative elongation of the front-to-back axis and substantially more lamellipodial ruffling, as compared to isolated cells. We have explored the effects of 2D confinement, separated from other in vivo environmental cues, by overlaying isolated cells with an agarose hydrogel with occasional spacers, or with a ceiling made of polydimethylsiloxane (PDMS) elastomer. Under these conditions, isolated keratocytes more closely resemble the in vivo migratory shape phenotype, displaying a flatter apical-basal axis and a longer front-to-back axis than unconfined keratocytes. We propose that 2D confinement contributes to multiple dimensions of in vivo keratocyte shape determination. Further analysis demonstrates that confinement causes a synchronous 20% decrease in both cell speed and volume. Interestingly, we were able to replicate the 20% decrease in speed using a sorbitol hypertonic shock to shrink the cell volume, which did not affect other aspects of cell shape. Collectively, our results suggest that environmentally imposed changes in cell volume may influence cell migration speed, potentially by perturbing physical properties of the cytoplasm." @default.
- W4312223721 created "2023-01-04" @default.
- W4312223721 creator A5000264279 @default.
- W4312223721 creator A5040234030 @default.
- W4312223721 creator A5078436996 @default.
- W4312223721 date "2023-01-01" @default.
- W4312223721 modified "2023-09-27" @default.
- W4312223721 title "Confined keratocytes mimic in vivo migration and reveal volume‐speed relationship" @default.
- W4312223721 cites W1505374435 @default.
- W4312223721 cites W1968272833 @default.
- W4312223721 cites W1972170345 @default.
- W4312223721 cites W1973537206 @default.
- W4312223721 cites W1976821656 @default.
- W4312223721 cites W1979033746 @default.
- W4312223721 cites W1983231438 @default.
- W4312223721 cites W1984474513 @default.
- W4312223721 cites W1989021197 @default.
- W4312223721 cites W1991547734 @default.
- W4312223721 cites W1993016783 @default.
- W4312223721 cites W2008063578 @default.
- W4312223721 cites W2008444291 @default.
- W4312223721 cites W2011376300 @default.
- W4312223721 cites W2026312110 @default.
- W4312223721 cites W2026967816 @default.
- W4312223721 cites W2029046412 @default.
- W4312223721 cites W2039727443 @default.
- W4312223721 cites W2043059009 @default.
- W4312223721 cites W2047903964 @default.
- W4312223721 cites W2052922251 @default.
- W4312223721 cites W2052973245 @default.
- W4312223721 cites W2060598691 @default.
- W4312223721 cites W2065071281 @default.
- W4312223721 cites W2083457899 @default.
- W4312223721 cites W2085542786 @default.
- W4312223721 cites W2087968576 @default.
- W4312223721 cites W2091401847 @default.
- W4312223721 cites W2099004980 @default.
- W4312223721 cites W2100324155 @default.
- W4312223721 cites W2101281572 @default.
- W4312223721 cites W2106217679 @default.
- W4312223721 cites W2107620022 @default.
- W4312223721 cites W2107750328 @default.
- W4312223721 cites W2111960078 @default.
- W4312223721 cites W2121858600 @default.
- W4312223721 cites W2125165341 @default.
- W4312223721 cites W2133210319 @default.
- W4312223721 cites W2137753785 @default.
- W4312223721 cites W2153071013 @default.
- W4312223721 cites W2161533644 @default.
- W4312223721 cites W2162751463 @default.
- W4312223721 cites W2166954447 @default.
- W4312223721 cites W2171727333 @default.
- W4312223721 cites W2268737993 @default.
- W4312223721 cites W2312179720 @default.
- W4312223721 cites W2325727928 @default.
- W4312223721 cites W2343169334 @default.
- W4312223721 cites W2402482764 @default.
- W4312223721 cites W2417770086 @default.
- W4312223721 cites W2525940887 @default.
- W4312223721 cites W2758251498 @default.
- W4312223721 cites W2759276314 @default.
- W4312223721 cites W2793855487 @default.
- W4312223721 cites W2870819341 @default.
- W4312223721 cites W2948980593 @default.
- W4312223721 cites W2950075392 @default.
- W4312223721 cites W2985428808 @default.
- W4312223721 cites W3000999925 @default.
- W4312223721 cites W3001129188 @default.
- W4312223721 cites W3001844098 @default.
- W4312223721 cites W3033687631 @default.
- W4312223721 cites W3094328328 @default.
- W4312223721 cites W3104572769 @default.
- W4312223721 cites W3107622347 @default.
- W4312223721 cites W3113111670 @default.
- W4312223721 cites W3129973863 @default.
- W4312223721 cites W3206785374 @default.
- W4312223721 cites W4200044074 @default.
- W4312223721 cites W4221064155 @default.
- W4312223721 cites W4225729315 @default.
- W4312223721 cites W4226136424 @default.
- W4312223721 cites W4226362602 @default.
- W4312223721 cites W4281393270 @default.
- W4312223721 cites W3149760511 @default.
- W4312223721 doi "https://doi.org/10.1002/cm.21741" @default.
- W4312223721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36576104" @default.
- W4312223721 hasPublicationYear "2023" @default.
- W4312223721 type Work @default.
- W4312223721 citedByCount "1" @default.
- W4312223721 countsByYear W43122237212023 @default.
- W4312223721 crossrefType "journal-article" @default.
- W4312223721 hasAuthorship W4312223721A5000264279 @default.
- W4312223721 hasAuthorship W4312223721A5040234030 @default.
- W4312223721 hasAuthorship W4312223721A5078436996 @default.
- W4312223721 hasBestOaLocation W43122237211 @default.
- W4312223721 hasConcept C105702510 @default.
- W4312223721 hasConcept C109185818 @default.
- W4312223721 hasConcept C12554922 @default.
- W4312223721 hasConcept C125705527 @default.