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- W4312652442 abstract "<b>Introduction:</b> Paediatric acute wheezing illness (AWI) airway models have not yet been established or characterised for intrinsic epithelial vulnerabilities, due to poor yield and culture survival. <b>Aim:</b> To establish and characterise a paediatric airway epithelial culture model of AWI. <b>Methods:</b> Nasal epithelial cells (NECs) sampled from children with AWI (n=6; 3 males; age 10.7±2.8) and community controls (n=6; 3 males; age 6.6±2.1) were cultured at air-liquid interface. Epithelial lineage markers (Cytokeratin (Ck)19, Ck5), vimentin, β-actin, β-tubulin, zonula occludens (ZO1) and rhinoviral receptors (ICAM1, LDLR, CDHR3) were assessed via qPCR. Barrier integrity and function was determined via trans-epithelial resistance and permeability assays. Basal inflammatory cytokine production (Interleukin (IL)6, -8) was measured via ELISA. <b>Results:</b> Similar gene expression was observed in both cohorts (AWI vs control (X ̅±SD) for Ck19 (2.88±1.21 vs 3.16±0.91), Ck5 (3.31±1.49 vs 3.51±1.78), vimentin (0.12±0.09 vs 0.15±0.10), β-actin (0.29±0.06 vs 0.28±0.06), ZO1 (0.0059±0.00089 vs 0.0054±0.0029), LDLR (0.69±0.32 vs 0.72±0.32), ICAM1 (0.23±0.24 vs 0.20±0.35) and CDHR3 (0.96±0.27 vs 1.61±0.94). β-tubulin was significantly lower the AWI cohort (7.17±2.56×10<sup>-6</sup> vs 2.27±1.37×10<sup>-5</sup>; p<0.05). Similar trans-epithelial resistance (Ω.cm<sup>2</sup>) (274.87±76.20 vs 302.60±135.65; n=5), permeability (cm/sec) (14.58±9.62×10<sup>-4</sup> vs 72.25±116.48×10<sup>-4</sup>; n=5) and basal inflammatory cytokines (pg/ml/µg protein) (IL6: 1.57±1.85, n=4 vs 0.52±0.21, n=3; IL8: 7.33±3.42; n=4 vs 4.05±1.49; n=3) were observed. <b>Conclusion:</b> We successfully established a well-differentiated culture model of children with AWI that can be used for future studies." @default.
- W4312652442 created "2023-01-05" @default.
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- W4312652442 date "2022-09-04" @default.
- W4312652442 modified "2023-10-14" @default.
- W4312652442 title "Establishing a paediatric airway epithelial model of acute wheezing illness" @default.
- W4312652442 doi "https://doi.org/10.1183/13993003.congress-2022.2235" @default.
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