Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312702295> ?p ?o ?g. }
- W4312702295 endingPage "3071" @default.
- W4312702295 startingPage "3061" @default.
- W4312702295 abstract "Silver has been used for its antimicrobial properties to fight infection for thousands of years. Unfortunately, some Gram-negative bacteria have developed silver resistance causing the death of patients in a burn unit. The genes responsible for silver resistance have been designated as the sil operon. Among the proteins of the sil operon, SilE has been shown to play a key role in bacterial silver resistance. Based on the limited information available, it has been depicted as an intrinsically disordered protein that folds into helices upon silver ion binding. Herein, this work demonstrates that SilE is composed of 4 clearly identified helical segments in the presence of several silver ions. The combination of analytical and biophysical techniques (NMR spectroscopy, CD, SAXS, HRMS, CE-ICP-MS, and IM-MS) reveals that SilE harbors four strong silver binding sites among the eight sites available. We have also further evidenced that SilE does not adopt a globular structure but rather samples a large conformational space from elongated to more compact structures. This particular structural organization facilitates silver binding through much higher accessibility of the involved His and Met residues. These valuable results will advance our current understanding of the role of SilE in the silver efflux pump complex mechanism and will help in the future rational design of inhibitors to fight bacterial silver resistance." @default.
- W4312702295 created "2023-01-05" @default.
- W4312702295 creator A5005494837 @default.
- W4312702295 creator A5006585346 @default.
- W4312702295 creator A5014551186 @default.
- W4312702295 creator A5018921214 @default.
- W4312702295 creator A5022744917 @default.
- W4312702295 creator A5030074467 @default.
- W4312702295 creator A5033924169 @default.
- W4312702295 creator A5054705052 @default.
- W4312702295 creator A5076808956 @default.
- W4312702295 creator A5083452724 @default.
- W4312702295 creator A5084156210 @default.
- W4312702295 date "2023-01-01" @default.
- W4312702295 modified "2023-10-18" @default.
- W4312702295 title "Structural and dynamical insights into SilE silver binding from combined analytical probes" @default.
- W4312702295 cites W1546794253 @default.
- W4312702295 cites W1972519948 @default.
- W4312702295 cites W1982126789 @default.
- W4312702295 cites W2019511555 @default.
- W4312702295 cites W2041819290 @default.
- W4312702295 cites W2044157441 @default.
- W4312702295 cites W2049747252 @default.
- W4312702295 cites W2077741051 @default.
- W4312702295 cites W2099584972 @default.
- W4312702295 cites W2156771958 @default.
- W4312702295 cites W2339536475 @default.
- W4312702295 cites W2346627216 @default.
- W4312702295 cites W2593245431 @default.
- W4312702295 cites W2613101576 @default.
- W4312702295 cites W2735621869 @default.
- W4312702295 cites W2791942866 @default.
- W4312702295 cites W2888018465 @default.
- W4312702295 cites W2898843313 @default.
- W4312702295 cites W2913434007 @default.
- W4312702295 cites W2968441064 @default.
- W4312702295 cites W3041634362 @default.
- W4312702295 cites W3132349200 @default.
- W4312702295 cites W3158082151 @default.
- W4312702295 cites W3177828909 @default.
- W4312702295 cites W3184227730 @default.
- W4312702295 cites W3195375135 @default.
- W4312702295 cites W4224214254 @default.
- W4312702295 cites W4280548618 @default.
- W4312702295 cites W4281790889 @default.
- W4312702295 cites W4285092858 @default.
- W4312702295 doi "https://doi.org/10.1039/d2cp04206a" @default.
- W4312702295 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36617868" @default.
- W4312702295 hasPublicationYear "2023" @default.
- W4312702295 type Work @default.
- W4312702295 citedByCount "1" @default.
- W4312702295 countsByYear W43127022952023 @default.
- W4312702295 crossrefType "journal-article" @default.
- W4312702295 hasAuthorship W4312702295A5005494837 @default.
- W4312702295 hasAuthorship W4312702295A5006585346 @default.
- W4312702295 hasAuthorship W4312702295A5014551186 @default.
- W4312702295 hasAuthorship W4312702295A5018921214 @default.
- W4312702295 hasAuthorship W4312702295A5022744917 @default.
- W4312702295 hasAuthorship W4312702295A5030074467 @default.
- W4312702295 hasAuthorship W4312702295A5033924169 @default.
- W4312702295 hasAuthorship W4312702295A5054705052 @default.
- W4312702295 hasAuthorship W4312702295A5076808956 @default.
- W4312702295 hasAuthorship W4312702295A5083452724 @default.
- W4312702295 hasAuthorship W4312702295A5084156210 @default.
- W4312702295 hasBestOaLocation W43127022951 @default.
- W4312702295 hasConcept C104317684 @default.
- W4312702295 hasConcept C105168689 @default.
- W4312702295 hasConcept C12554922 @default.
- W4312702295 hasConcept C185592680 @default.
- W4312702295 hasConcept C203075996 @default.
- W4312702295 hasConcept C547475151 @default.
- W4312702295 hasConcept C55493867 @default.
- W4312702295 hasConcept C8010536 @default.
- W4312702295 hasConcept C86803240 @default.
- W4312702295 hasConcept C89423630 @default.
- W4312702295 hasConceptScore W4312702295C104317684 @default.
- W4312702295 hasConceptScore W4312702295C105168689 @default.
- W4312702295 hasConceptScore W4312702295C12554922 @default.
- W4312702295 hasConceptScore W4312702295C185592680 @default.
- W4312702295 hasConceptScore W4312702295C203075996 @default.
- W4312702295 hasConceptScore W4312702295C547475151 @default.
- W4312702295 hasConceptScore W4312702295C55493867 @default.
- W4312702295 hasConceptScore W4312702295C8010536 @default.
- W4312702295 hasConceptScore W4312702295C86803240 @default.
- W4312702295 hasConceptScore W4312702295C89423630 @default.
- W4312702295 hasFunder F4320320883 @default.
- W4312702295 hasFunder F4320322892 @default.
- W4312702295 hasIssue "4" @default.
- W4312702295 hasLocation W43127022951 @default.
- W4312702295 hasLocation W43127022952 @default.
- W4312702295 hasOpenAccess W4312702295 @default.
- W4312702295 hasPrimaryLocation W43127022951 @default.
- W4312702295 hasRelatedWork W1824498722 @default.
- W4312702295 hasRelatedWork W184116023 @default.
- W4312702295 hasRelatedWork W2097794149 @default.
- W4312702295 hasRelatedWork W2129151986 @default.
- W4312702295 hasRelatedWork W2132590114 @default.
- W4312702295 hasRelatedWork W2153122502 @default.