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- W4312776244 abstract "Urease is an enzyme that catalyzes the hydrolysis reaction of urea to ammonia and carbon dioxide. Urease used in this study extracted from the seeds of kidney beans (Phaseolus vulgaris L). The efficiency of urease increased by immobilization of the enzyme using a chitosan-bentonite matrix. This research aims to increase the effectiveness of urease from bean kidney by immobilization using chitosan bentonite. The preliminary of the study was urease extraction from kidney beans at various germination times. The urease activity was determined using Nessler's reagent and characterized by a spectrophotometer. Urease with optimum activity was obtained by kidney bean seed with a germination time of 8 days. Urease immobilization showed optimum conditions at a bentonite concentration of 3% (w/v) of the total volume of beads, TPP 3% (w/v), and immersion time of 60 minutes. The characterization results showed that free urease has a pH optimum of 7, the temperature of 35 °C, KM value of 4.46 mM, and Vmax of 25.25 mM/min. Immobilized Urease has optimum pH 7, the temperature of 40 °C, KM value of 5.80 mM, and Vmax of 9.50 mM/min. Immobilized urease can be used up to 4 times, and its activity persisted 75.8% after eight days of storage" @default.
- W4312776244 created "2023-01-05" @default.
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- W4312776244 date "2022-01-01" @default.
- W4312776244 modified "2023-10-01" @default.
- W4312776244 title "Immobilization and characterization of urease from Phaseolus vulgaris L using bentonite chitosan matrix" @default.
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- W4312776244 doi "https://doi.org/10.1063/5.0103676" @default.
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