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- W4312908123 abstract "<b>Objectives-aim:</b> Culture-independent techniques show the composition of airway microbiota(AM) in primary ciliary dyskinesia (PCD) patients. Nongenetic factors might influence the composition of AM of PCD patients. We aimed to show the composition and structure of AM in PCD and explore the affected factor on AM of PCD patients. <b>Material and methods:</b> A cross-sectional study was performed. We recruited 14 PCD patients (7pairs siblings) and 9 family members. Bacterial rDNA was extracted from sputum and nasal samples. Sputum samples were also inoculated on suitable bacteriological media. <b>Results:</b> 33 separate genera were detected in the sputum sample of PCD patients, and 41 different genera were in the nasal samples of family member(Fig1). There were no significant differences in alpha diversity between each of the siblings’ pairs and siblings’ samples did not cluster together nearly as strongly as non-siblings’ samples according to Bray-Curtis Dissimilarity analysis. There was no significant AM diversity between PCD siblings’ pair and their family members. Patients who had no complaints and bacterial grown on culture-dependent method at the time of the study sampling and patients who had no at least one H. influenza grew in the last year had a significantly greater diversity(p<0.05). Microbiota communities were tended to cluster together by age pulmonary exacerbation status during the last year, having at least one H. influenza grown on culture-dependent analyses during the last year, FEV1z score(Fig2). <b>Conclusion:</b> The AM of PCD patients might influenced by clinical charecteristics of patients. Siblings pair has not more comminity similarities than non-sibling PCD." @default.
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- W4312908123 date "2022-09-04" @default.
- W4312908123 modified "2023-10-16" @default.
- W4312908123 title "The airway microbiota in siblings with primary ciliary dyskinesia: related factors and correlation with clinical characteristics" @default.
- W4312908123 doi "https://doi.org/10.1183/13993003.congress-2022.3579" @default.
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