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- W4312909391 abstract "During secondary growth in trees, xylem cells differentiated from cambium cells begin to synthesize secondary cell walls that are primarily composed of cellulose, hemicellulose and lignin, and are deposited between primary cell walls and plasma membranes, leading to in wood formation. Identification of regulatory genes functioning during this developmental process is valuable for increasing wood production. In this study, we functionally characterized an LBD (LATERAL ORGAN BOUNDARIES DOMAIN) transcription factor PagLBD21 as a repressor of secondary xylem development in <italic>Populus</italic>. Compared to wild type plants, transgenic plants overexpressing <italic>PagLBD21</italic> (<italic>PagLBD21OE</italic>) exhibited decreased xylem widths in cross-sections. Consistent with the functional analysis, RNA sequencing (RNA-seq) analysis revealed that genes functioning in xylem development and secondary cell wall biosynthesis pathways were significantly down-regulated in <italic>PagLBD21OE</italic> plants. We also performed DNA affinity purification followed by sequencing (DAP-seq) to identify genome-wide target genes of PagLBD21. Furthermore, we compared the RNA-seq and DAP-seq datasets of PagLBD21 and PagLBD3, and the results showed that there was a significant overlap between their target genes, suggesting these two LBD transcription factors are functionally redundant during secondary growth." @default.
- W4312909391 created "2023-01-05" @default.
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- W4312909391 date "2022-01-01" @default.
- W4312909391 modified "2023-09-28" @default.
- W4312909391 title "Transcription factor PagLBD21 functions as a repressor of secondary xylem development in <i>Populus</i>" @default.
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- W4312909391 doi "https://doi.org/10.48130/fr-2022-0019" @default.
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