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- W4312927744 abstract "High IL-33 level can be observed within the airways of asthmatics. Moreover, both IL-33 and ST2/IL1RL1 genes were identified as major asthma susceptibility loci in genome-wide association studies. A relevant role in allergic asthma associated airways remodelling events is also played by eosinophils derived exosomes. Aim of present study is demonstrate that TC-1 spheroids are a useful tool to study eosinophil-mediated loss of epithelial integrity associated with airway remodelling events through a recapitulation of cell crosstalk process. Murine eosinophils were differentiated from bone-marrow and activated with IL-33 as already described. TC-1 lung carcinoma cells (provided by G.Kroemer, Gustave Roussy Cancer Institute, Villejuif, France) were cultured in ultra-low attachment plates to form spheroid structures. Granules (G-33) and exosomes (EXO-33) were purified from eosinophil culture supernatants by serial ultracentrifugations. Conditioned medium (CM) from eosinophils activated with IL-33 (EOs-33) was able to disaggregate TC-1 spheroids, resembling respiratory epithelium flanking associated with airway chronic inflammation and remodelling events. Moreover, both G-33 and EXO-33 induced TC-1 spheroid disruption. Importantly, allergen treated TC-1 cells expressed higher levels of the IL-33, IL-13 and EOs attractive chemokines with respect to untreated cells. Our data agree with Cañas J.A. et al.(PMID: 29451337)with further indication that allergen driven IL-33 induce EXO-33 mediated lung epithelium damage. Finally, exposure of respiratory epithelium to cysteine-protease airborne allergens was sufficient to start remodelling events through eosinophils recruitment and activation." @default.
- W4312927744 created "2023-01-05" @default.
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- W4312927744 date "2022-09-04" @default.
- W4312927744 modified "2023-09-25" @default.
- W4312927744 title "Eosinophil-epithelial respiratory cells crosstalk in IL-33 mediated inflammation 3D models." @default.
- W4312927744 doi "https://doi.org/10.1183/13993003.congress-2022.176" @default.
- W4312927744 hasPublicationYear "2022" @default.
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