Matches in SemOpenAlex for { <https://semopenalex.org/work/W4312964110> ?p ?o ?g. }
- W4312964110 endingPage "129" @default.
- W4312964110 startingPage "121" @default.
- W4312964110 abstract "Ubiquitin and related ubiquitin-like proteins (Ubls) influence a variety of cellular pathways including protein degradation and response to viral infections. The chemical interrogation of these complex enzymatic cascades relies on the use of tailored activity-based probes (ABPs). Herein, we report the preparation of ABPs for ubiquitin, NEDD8, SUMO2 and ISG15 by selective acyl hydrazide modification. Acyl hydrazides of Ubls are readily accessible by direct hydrazinolysis of Ubl-intein fusions. The suppressed pKa and superior nucleophilicity of the acyl hydrazides enables their selective modification at acidic pH with carboxylic acid anhydrides. The modification proceeds rapidly and efficiently, and does not require chromatographic purification or refolding of the probes. We modified Ubl-NHNH2 with various thiol-reactive electrophiles that couple selectively with E2s and DUBs. The ease of modification enables the rapid generation and screening of ubiquitin probes with various C-terminal truncations and warheads for the selection of the most suitable combination for a given E2 or DUB." @default.
- W4312964110 created "2023-01-05" @default.
- W4312964110 creator A5043512257 @default.
- W4312964110 creator A5051549888 @default.
- W4312964110 creator A5058457451 @default.
- W4312964110 date "2023-01-01" @default.
- W4312964110 modified "2023-09-30" @default.
- W4312964110 title "Installation of electrophiles onto the C-terminus of recombinant ubiquitin and ubiquitin-like proteins" @default.
- W4312964110 cites W1482991332 @default.
- W4312964110 cites W1983470920 @default.
- W4312964110 cites W1987428238 @default.
- W4312964110 cites W2001234769 @default.
- W4312964110 cites W2001462701 @default.
- W4312964110 cites W2003190991 @default.
- W4312964110 cites W2021670268 @default.
- W4312964110 cites W2024431726 @default.
- W4312964110 cites W2029837691 @default.
- W4312964110 cites W2030746003 @default.
- W4312964110 cites W2039192919 @default.
- W4312964110 cites W2041893317 @default.
- W4312964110 cites W2045156375 @default.
- W4312964110 cites W2050574817 @default.
- W4312964110 cites W2054934898 @default.
- W4312964110 cites W2065455501 @default.
- W4312964110 cites W2068621468 @default.
- W4312964110 cites W2077494922 @default.
- W4312964110 cites W2086424573 @default.
- W4312964110 cites W2099598643 @default.
- W4312964110 cites W2110960544 @default.
- W4312964110 cites W2112074525 @default.
- W4312964110 cites W2132249118 @default.
- W4312964110 cites W2136133297 @default.
- W4312964110 cites W2143323618 @default.
- W4312964110 cites W2152192156 @default.
- W4312964110 cites W2152991917 @default.
- W4312964110 cites W2158359547 @default.
- W4312964110 cites W2166987747 @default.
- W4312964110 cites W2327328750 @default.
- W4312964110 cites W2400942913 @default.
- W4312964110 cites W2529962815 @default.
- W4312964110 cites W2590813565 @default.
- W4312964110 cites W2613065881 @default.
- W4312964110 cites W2718524622 @default.
- W4312964110 cites W2793986986 @default.
- W4312964110 cites W2891136419 @default.
- W4312964110 cites W2998745527 @default.
- W4312964110 cites W3047344569 @default.
- W4312964110 cites W3111133807 @default.
- W4312964110 cites W3152199349 @default.
- W4312964110 cites W3156921472 @default.
- W4312964110 cites W4280556587 @default.
- W4312964110 doi "https://doi.org/10.1039/d2sc04279g" @default.
- W4312964110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36605735" @default.
- W4312964110 hasPublicationYear "2023" @default.
- W4312964110 type Work @default.
- W4312964110 citedByCount "3" @default.
- W4312964110 countsByYear W43129641102023 @default.
- W4312964110 crossrefType "journal-article" @default.
- W4312964110 hasAuthorship W4312964110A5043512257 @default.
- W4312964110 hasAuthorship W4312964110A5051549888 @default.
- W4312964110 hasAuthorship W4312964110A5058457451 @default.
- W4312964110 hasBestOaLocation W43129641101 @default.
- W4312964110 hasConcept C100631289 @default.
- W4312964110 hasConcept C104317684 @default.
- W4312964110 hasConcept C134459356 @default.
- W4312964110 hasConcept C146727910 @default.
- W4312964110 hasConcept C161790260 @default.
- W4312964110 hasConcept C178790620 @default.
- W4312964110 hasConcept C181199279 @default.
- W4312964110 hasConcept C184133267 @default.
- W4312964110 hasConcept C185592680 @default.
- W4312964110 hasConcept C21951064 @default.
- W4312964110 hasConcept C25602115 @default.
- W4312964110 hasConcept C2775954774 @default.
- W4312964110 hasConcept C2775998212 @default.
- W4312964110 hasConcept C2779201268 @default.
- W4312964110 hasConcept C2779231659 @default.
- W4312964110 hasConcept C2780718723 @default.
- W4312964110 hasConcept C2911199164 @default.
- W4312964110 hasConcept C50027330 @default.
- W4312964110 hasConcept C54458228 @default.
- W4312964110 hasConcept C55493867 @default.
- W4312964110 hasConcept C67705224 @default.
- W4312964110 hasConceptScore W4312964110C100631289 @default.
- W4312964110 hasConceptScore W4312964110C104317684 @default.
- W4312964110 hasConceptScore W4312964110C134459356 @default.
- W4312964110 hasConceptScore W4312964110C146727910 @default.
- W4312964110 hasConceptScore W4312964110C161790260 @default.
- W4312964110 hasConceptScore W4312964110C178790620 @default.
- W4312964110 hasConceptScore W4312964110C181199279 @default.
- W4312964110 hasConceptScore W4312964110C184133267 @default.
- W4312964110 hasConceptScore W4312964110C185592680 @default.
- W4312964110 hasConceptScore W4312964110C21951064 @default.
- W4312964110 hasConceptScore W4312964110C25602115 @default.
- W4312964110 hasConceptScore W4312964110C2775954774 @default.
- W4312964110 hasConceptScore W4312964110C2775998212 @default.
- W4312964110 hasConceptScore W4312964110C2779201268 @default.
- W4312964110 hasConceptScore W4312964110C2779231659 @default.