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- W4313019128 startingPage "1333" @default.
- W4313019128 abstract "The combination of heart-specific transcription factors GATA4, MEF2C, and TBX5 (GMT) has been proven to have the ability to directly reprogram cardiac fibroblasts; this approach is considered a promising regenerative technique. Meanwhile, research on microbubbles as biological vectors has made great progress in recent years. This study describes the loading of GMT lentiviral vectors on cationic microbubbles and the release of these direct-reprogramming vectors into an infarcted myocardium by ultrasound targeted microbubble destruction (UTMD) to repair the cardiac tissue. Lentivirus which encode GATA4, MEF2C, and TBX5 transcription factors were generated via a lentiviral production system and were confirmed to have a direct reprogramming ability in vitro. Combined with the cationic microbubbles, UTMD-mediated gene delivery was evaluated, and the gene transfection efficiency was optimized in an in vitro experiment on rat cardiac fibroblasts. With UTMD-mediated direct reprogramming, the viral vector particles were directly deposited in cardiac tissue and repaired the infarcted myocardium. An immunofluorescence assay and histological examination confirmed newborn cardiomyocytes and neo-angiogenesis after a 4-week follow-up of the treated rats. All treated groups showed ventricular-function improvement according to cardiac magnetic resonance imaging and echocardiography. This chapter reports a novel strategy for the delivery of direct-reprogramming lentiviral vectors to a target acute myocardial infarction zone by using UTMD as a tissue repair therapy." @default.
- W4313019128 created "2023-01-05" @default.
- W4313019128 creator A5033702023 @default.
- W4313019128 creator A5051109032 @default.
- W4313019128 creator A5087456408 @default.
- W4313019128 date "2022-01-01" @default.
- W4313019128 modified "2023-09-30" @default.
- W4313019128 title "Bioengineering Technique Progress of Direct Cardiac Reprogramming" @default.
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