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- W4313186775 abstract "Cryopreservation of germplasm in liquid nitrogen is an ideal technique for the longer term storage of plant genetic material, including medicinal species.To develop a somatic embryo production system for the medicinal species Swertia chirayita and to evaluate their potential for storage in liquid nitrogen (-196 degree C).An efficient protocol of somatic embryogenesis was developed for the first time using leaves of in-vitro grown shoots of S. chirayita. Somatic embryos were then encapsulated in 3% sodium alginate, 0.85 M sucrose and 100 mM calcium chloride for synthetic seed production and subjected to cryopreservation. Marker medicinal compounds were determined by RP-HPLC analysis.A medium containing 1 mg/L 2,4-D+ 0.5 mg/L BAP+ 0.5 mg/L TDZ was found to stimulate the highest callus induction. Somatic embryos were recovered after 5 weeks, when cultured on the same media. Synthetic seeds were dehydrated and immersed in liquid nitrogen for 1 h. Cryopreserved synthetic seeds were successfully revived and germinated on MS media supplemented with 1 mg/L IBA+ 2 mg/L KN + 3 mg/L GA3 in which 93.3% somatic embryos differentiated into shoots. One month old in-vitro grown shoots from cryopreserved somatic embryos had similar marker medicinal compounds, such as amarogentin (4.72 ± 0.11 ug/mg) and mangiferin (14.54 ± 0.05 ug/mg), as control material.This protocol offers vast scope for multiplying material of an endangered medicinal herb and subsequent cryopreservation. doi.org/10.54680/fr22510110612." @default.
- W4313186775 created "2023-01-06" @default.
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- W4313186775 date "2022-09-01" @default.
- W4313186775 modified "2023-09-26" @default.
- W4313186775 title "Artificial Seed Production and Cryopreservation by Encapsulation Dehydration for Medicinal Herb of Himalayan Region,<i> Swertia Chirayita</i>" @default.
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- W4313186775 doi "https://doi.org/10.54680/fr22510110612" @default.
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