Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313189845> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W4313189845 abstract "Background: Human embryonic stem cell-derived epicardium (hESC-Epis) has been shown to improve the function of hESC-derived cardiomyocytes (hESC-CM) in vitro and post-transplantation into animal myocardial infarction models. However, the underlying mechanisms mediating these effects remain poorly characterised. Bulk RNA sequencing of hESC-Epis suggests a role for genes related to extracellular matrix remodeling in mediating these effects, with fibronectin (FN1) being highly implicated. Study Objective: To examine the role of FN1 in hESC-CM/Epi crosstalk through gain and loss of function studies. Methods: A tetracycline inducible FN1 knockdown system was used on hESC-CMs and Epis within the setting of 3D-engineered heart tissue. Changes in contractility and calcium handling were measured. Recombinant human plasma FN1 (rhFN) was then used to determine the effect of FN1 supplementation on hESC-CM function. Results: FN1 knockdown in hESC-Epis resulted in significant abrogation of the beneficial effects on hESC-CM force generation and calcium handling. Knockdown of FN1 in hESC-CMs however significantly attenuated contractility of the hESC-CMs but had no effects on calcium handling. When rhFN was added to hESC-CMs in increasing doses, hESC-CM contractility was improved in a dose dependent fashion to the same extent as seen with hESC-Epis co-cultures. However, rhFN supplementation did not result in significant improvements in calcium handling. Conclusions: hESC-CM and Epi crosstalk improving hESC-CM function may rely heavily on the presence of FN1. FN1 secreted by both cell types are shown to be of importance in this crosstalk with artificial supplementation of rhFN being able to recapitulate some beneficial effects of hESC-Epis on hESC-CMs. Further studies are required to determine the role of specific isoforms of secreted FN1 on hESC-CM maturity and what mechanism underlies these effects. This opens up the possibility of improving cell-based therapy for heart failure post-myocardial infarction using recombinant FN1 supplementation." @default.
- W4313189845 created "2023-01-06" @default.
- W4313189845 creator A5005964345 @default.
- W4313189845 creator A5015597755 @default.
- W4313189845 creator A5021259283 @default.
- W4313189845 creator A5044067769 @default.
- W4313189845 creator A5048692866 @default.
- W4313189845 creator A5062626079 @default.
- W4313189845 creator A5063350096 @default.
- W4313189845 creator A5071460486 @default.
- W4313189845 creator A5072776448 @default.
- W4313189845 date "2022-08-05" @default.
- W4313189845 modified "2023-10-17" @default.
- W4313189845 title "Abstract P1015: Construction Of A Pro-cardiogenic Extracellular Matrix: How Stem-cell Derived Epicardium Enhances Cardiac Regeneration Through Fibronectin" @default.
- W4313189845 doi "https://doi.org/10.1161/res.131.suppl_1.p1015" @default.
- W4313189845 hasPublicationYear "2022" @default.
- W4313189845 type Work @default.
- W4313189845 citedByCount "0" @default.
- W4313189845 crossrefType "journal-article" @default.
- W4313189845 hasAuthorship W4313189845A5005964345 @default.
- W4313189845 hasAuthorship W4313189845A5015597755 @default.
- W4313189845 hasAuthorship W4313189845A5021259283 @default.
- W4313189845 hasAuthorship W4313189845A5044067769 @default.
- W4313189845 hasAuthorship W4313189845A5048692866 @default.
- W4313189845 hasAuthorship W4313189845A5062626079 @default.
- W4313189845 hasAuthorship W4313189845A5063350096 @default.
- W4313189845 hasAuthorship W4313189845A5071460486 @default.
- W4313189845 hasAuthorship W4313189845A5072776448 @default.
- W4313189845 hasConcept C104317684 @default.
- W4313189845 hasConcept C120665830 @default.
- W4313189845 hasConcept C121332964 @default.
- W4313189845 hasConcept C126322002 @default.
- W4313189845 hasConcept C134018914 @default.
- W4313189845 hasConcept C145103041 @default.
- W4313189845 hasConcept C169822122 @default.
- W4313189845 hasConcept C173396325 @default.
- W4313189845 hasConcept C189165786 @default.
- W4313189845 hasConcept C190283241 @default.
- W4313189845 hasConcept C39133596 @default.
- W4313189845 hasConcept C55493867 @default.
- W4313189845 hasConcept C71924100 @default.
- W4313189845 hasConcept C86492073 @default.
- W4313189845 hasConcept C86803240 @default.
- W4313189845 hasConcept C95444343 @default.
- W4313189845 hasConceptScore W4313189845C104317684 @default.
- W4313189845 hasConceptScore W4313189845C120665830 @default.
- W4313189845 hasConceptScore W4313189845C121332964 @default.
- W4313189845 hasConceptScore W4313189845C126322002 @default.
- W4313189845 hasConceptScore W4313189845C134018914 @default.
- W4313189845 hasConceptScore W4313189845C145103041 @default.
- W4313189845 hasConceptScore W4313189845C169822122 @default.
- W4313189845 hasConceptScore W4313189845C173396325 @default.
- W4313189845 hasConceptScore W4313189845C189165786 @default.
- W4313189845 hasConceptScore W4313189845C190283241 @default.
- W4313189845 hasConceptScore W4313189845C39133596 @default.
- W4313189845 hasConceptScore W4313189845C55493867 @default.
- W4313189845 hasConceptScore W4313189845C71924100 @default.
- W4313189845 hasConceptScore W4313189845C86492073 @default.
- W4313189845 hasConceptScore W4313189845C86803240 @default.
- W4313189845 hasConceptScore W4313189845C95444343 @default.
- W4313189845 hasIssue "Suppl_1" @default.
- W4313189845 hasLocation W43131898451 @default.
- W4313189845 hasOpenAccess W4313189845 @default.
- W4313189845 hasPrimaryLocation W43131898451 @default.
- W4313189845 hasRelatedWork W1590619267 @default.
- W4313189845 hasRelatedWork W1993602817 @default.
- W4313189845 hasRelatedWork W1995698795 @default.
- W4313189845 hasRelatedWork W2034939167 @default.
- W4313189845 hasRelatedWork W2064487804 @default.
- W4313189845 hasRelatedWork W2106761248 @default.
- W4313189845 hasRelatedWork W2130809134 @default.
- W4313189845 hasRelatedWork W2330163142 @default.
- W4313189845 hasRelatedWork W2414312069 @default.
- W4313189845 hasRelatedWork W2489907708 @default.
- W4313189845 hasVolume "131" @default.
- W4313189845 isParatext "false" @default.
- W4313189845 isRetracted "false" @default.
- W4313189845 workType "article" @default.