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- W4313198908 abstract "Recent study showed that cannabinoid CB1 receptor-mediated signaling may take a huge role in age-related modification of brain function. CB1 deficient animals show significant cognitive deficit in old age, however they show better learning ability in young age. Even though the finding is surprising, the molecular mechanisms of such difference, especially for better learning in young age remains unknown mainly due to lack of suitable in-vivo/ex-vivo models. Here we will purpose to use the primary cultured neurons as the ex-vivo model of aging, which can be enabled with gentleMACS technology. This method is said to enable the primary cell culture from adult rodents but up to age of P60. In the present study we aimed to establish a method for primary culture of brain neurons using aged mice, which can be also used for knockout animals of CB1 receptors." @default.
- W4313198908 created "2023-01-06" @default.
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- W4313198908 date "2022-01-01" @default.
- W4313198908 modified "2023-10-16" @default.
- W4313198908 title "Establishment of the novel method to culture primary neurons from aged rodent brain" @default.
- W4313198908 doi "https://doi.org/10.1254/jpssuppl.96.0_2-b-p-115" @default.
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