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- W4313199162 abstract "Lymphoid tissue stromal cells are important regulators of spleen homeostasis and immune responses. Here, we present an optimized protocol that describes the digestion and enrichment steps for the isolation and analysis of rare populations of stromal cells, including fibroblastic reticular cells, perivascular cells, and glial cells found in the spleen. This protocol is suitable for subsequent analysis of spleen stromal cells by flow cytometry or single-cell RNA sequencing and to analyze different disease models. For complete details on the use and execution of this protocol, please refer to Alexandre et al. (2022).1." @default.
- W4313199162 created "2023-01-06" @default.
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- W4313199162 date "2022-12-01" @default.
- W4313199162 modified "2023-10-14" @default.
- W4313199162 title "An optimized protocol for the isolation of rare stromal cell populations from the mouse spleen" @default.
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- W4313199162 doi "https://doi.org/10.1016/j.xpro.2022.101923" @default.
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