FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313202340> ?p ?o ?g. }

• W4313202340 endingPage "24" @default.
• W4313202340 startingPage "15" @default.
• W4313202340 abstract "Dextran α-1,2-debranching enzyme (DDE) releases glucose with hydrolyzing α-(1→2)-glucosidic linkages in α-glucans, which are made up of dextran with α-(1→2)-branches and are generated by Leuconostoc bacteria. DDE was isolated from Microbacterium dextranolyticum (formerly known as Flavobacterium sp. M-73) 40 years ago, although the amino acid sequence of the enzyme has not been determined. Herein, we found a gene for this enzyme based on the partial amino acid sequences from native DDE and characterized the recombinant enzyme. DDE had a signal peptide, a glycoside hydrolase family 65 domain, a carbohydrate-binding module family 35 domain, a domain (D-domain) similar to the C-terminal domain of Arthrobacter globiformis glucodextranase, and a transmembrane region at the C-terminus. Recombinant DDE released glucose from α-(1→2)-branched α-glucans produced by Leuconostoc citreum strains B-1299, S-32, and S-64 and showed weak hydrolytic activity with kojibiose and kojitriose. No activity was detected for commercial dextran and Leuconostoc citreum B-1355 α-glucan, which do not contain α-(1→2)-linkages. The removal of the D-domain decreased the affinity for α-(1→2)-branched α-glucans but not for kojioligosaccharides, suggesting that D-domain plays a role in α-glucan binding. Genes for putative dextranases, oligo-1,6-glucosidase, sugar-binding protein, and permease were present in the vicinity of the DDE gene, and as a result these gene products may be necessary for the use of α-(1→2)-branched glucans. Our findings shed new light on how actinobacteria utilize polysaccharides produced by lactic acid bacteria." @default.
• W4313202340 created "2023-01-06" @default.
• W4313202340 creator A5017379624 @default.
• W4313202340 creator A5018854394 @default.
• W4313202340 creator A5019721738 @default.
• W4313202340 creator A5058663905 @default.
• W4313202340 creator A5063321504 @default.
• W4313202340 date "2023-03-03" @default.
• W4313202340 modified "2023-10-18" @default.
• W4313202340 title "Identification and Characterization of Dextran α-1,2-Debranching Enzyme from <i>Microbacterium dextranolyticum</i>" @default.
• W4313202340 cites W1553947817 @default.
• W4313202340 cites W1820886481 @default.
• W4313202340 cites W1935712031 @default.
• W4313202340 cites W1963542991 @default.
• W4313202340 cites W1966234186 @default.
• W4313202340 cites W1970695165 @default.
• W4313202340 cites W1971887998 @default.
• W4313202340 cites W1972534901 @default.
• W4313202340 cites W1976745215 @default.
• W4313202340 cites W1983562607 @default.
• W4313202340 cites W1986171829 @default.
• W4313202340 cites W1988966516 @default.
• W4313202340 cites W1998405799 @default.
• W4313202340 cites W2000749077 @default.
• W4313202340 cites W2014834146 @default.
• W4313202340 cites W2021347037 @default.
• W4313202340 cites W2026279794 @default.
• W4313202340 cites W2026372724 @default.
• W4313202340 cites W2030714091 @default.
• W4313202340 cites W2033248541 @default.
• W4313202340 cites W2037484215 @default.
• W4313202340 cites W2042112590 @default.
• W4313202340 cites W2068693823 @default.
• W4313202340 cites W2076646298 @default.
• W4313202340 cites W2078516832 @default.
• W4313202340 cites W2082168776 @default.
• W4313202340 cites W2083478956 @default.
• W4313202340 cites W2085683856 @default.
• W4313202340 cites W2105734162 @default.
• W4313202340 cites W2115046947 @default.
• W4313202340 cites W2121996283 @default.
• W4313202340 cites W2154472869 @default.
• W4313202340 cites W2157275521 @default.
• W4313202340 cites W2165668860 @default.
• W4313202340 cites W2168974130 @default.
• W4313202340 cites W2194510134 @default.
• W4313202340 cites W2313142464 @default.
• W4313202340 cites W2318036417 @default.
• W4313202340 cites W2330140848 @default.
• W4313202340 cites W2362942253 @default.
• W4313202340 cites W2590326703 @default.
• W4313202340 cites W2744475623 @default.
• W4313202340 cites W2767710488 @default.
• W4313202340 cites W2912990441 @default.
• W4313202340 cites W2964435849 @default.
• W4313202340 cites W2969655821 @default.
• W4313202340 cites W2976010478 @default.
• W4313202340 cites W2991012415 @default.
• W4313202340 cites W3025481076 @default.
• W4313202340 cites W3045586587 @default.
• W4313202340 cites W3094967361 @default.
• W4313202340 cites W3177828909 @default.
• W4313202340 cites W3205476802 @default.
• W4313202340 cites W3211004843 @default.
• W4313202340 cites W3217216416 @default.
• W4313202340 cites W4223552703 @default.
• W4313202340 cites W4250326364 @default.
• W4313202340 cites W4250801064 @default.
• W4313202340 cites W4255712764 @default.
• W4313202340 cites W4255745823 @default.
• W4313202340 cites W4281790889 @default.
• W4313202340 doi "https://doi.org/10.5458/jag.jag.jag-2022_0013" @default.
• W4313202340 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37033117" @default.
• W4313202340 hasPublicationYear "2023" @default.
• W4313202340 type Work @default.
• W4313202340 citedByCount "3" @default.
• W4313202340 countsByYear W43132023402023 @default.
• W4313202340 crossrefType "journal-article" @default.
• W4313202340 hasAuthorship W4313202340A5017379624 @default.
• W4313202340 hasAuthorship W4313202340A5018854394 @default.
• W4313202340 hasAuthorship W4313202340A5019721738 @default.
• W4313202340 hasAuthorship W4313202340A5058663905 @default.
• W4313202340 hasAuthorship W4313202340A5063321504 @default.
• W4313202340 hasBestOaLocation W43132023401 @default.
• W4313202340 hasConcept C100544194 @default.
• W4313202340 hasConcept C181199279 @default.
• W4313202340 hasConcept C185592680 @default.
• W4313202340 hasConcept C196032511 @default.
• W4313202340 hasConcept C2776183398 @default.
• W4313202340 hasConcept C2778859111 @default.
• W4313202340 hasConcept C2780206646 @default.
• W4313202340 hasConcept C55493867 @default.
• W4313202340 hasConceptScore W4313202340C100544194 @default.
• W4313202340 hasConceptScore W4313202340C181199279 @default.
• W4313202340 hasConceptScore W4313202340C185592680 @default.
• W4313202340 hasConceptScore W4313202340C196032511 @default.
• W4313202340 hasConceptScore W4313202340C2776183398 @default.
• W4313202340 hasConceptScore W4313202340C2778859111 @default.

• next