FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313203764> ?p ?o ?g. }

• W4313203764 endingPage "e0279551" @default.
• W4313203764 startingPage "e0279551" @default.
• W4313203764 abstract "The objectives of this study were to evaluate the effect of perfluoroalkyl substances on early embryonic development and apoptosis in blastocysts using a porcine in vitro model. Porcine oocytes (N = 855) collected from abattoir ovaries were subjected to perfluorooctane sulfonic acid (PFOS) (0.1 μg/ml) and perfluorohexane sulfonic acid (PFHxS) (40 μg/ml) during in vitro maturation (IVM) for 45 h. The gametes were then fertilized and cultured in vitro, and developmental parameters were recorded. After 6 days of culture, resulting blastocysts (N = 146) were stained using a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay and imaged as stacks using confocal laser scanning microscopy. Proportion of apoptotic cells as well as total numbers of nuclei in each blastocyst were analyzed using objective image analysis. The experiment was run in 9 replicates, always with a control present. Effects on developmental parameters were analyzed using logistic regression, and effects on apoptosis and total numbers of nuclei were analyzed using linear regression. Higher cell count was associated with lower proportion of apoptotic cells, i.e., larger blastocysts contained less apoptotic cells. Upon PFAS exposure during IVM, PFHxS tended to result in higher blastocyst rates on day 5 post fertilization (p = 0.07) and on day 6 post fertilization (p = 0.05) as well as in higher apoptosis rates in blastocysts (p = 0.06). PFHxS resulted in higher total cell counts in blastocysts (p = 0.002). No effects attributable to the concentration of PFOS used here was seen. These findings add to the evidence that some perfluoroalkyl substances may affect female reproduction. More studies are needed to better understand potential implications for continued development as well as for human health." @default.
• W4313203764 created "2023-01-06" @default.
• W4313203764 creator A5005630190 @default.
• W4313203764 creator A5037799592 @default.
• W4313203764 creator A5075216909 @default.
• W4313203764 creator A5086969446 @default.
• W4313203764 date "2022-12-28" @default.
• W4313203764 modified "2023-09-26" @default.
• W4313203764 title "Occurrence of late-apoptotic symptoms in porcine preimplantation embryos upon exposure of oocytes to perfluoroalkyl substances (PFASs) under in vitro meiotic maturation" @default.
• W4313203764 cites W1965296879 @default.
• W4313203764 cites W1976770880 @default.
• W4313203764 cites W1986194759 @default.
• W4313203764 cites W1996863157 @default.
• W4313203764 cites W2025315910 @default.
• W4313203764 cites W2048919588 @default.
• W4313203764 cites W2057310849 @default.
• W4313203764 cites W2061648581 @default.
• W4313203764 cites W2066181078 @default.
• W4313203764 cites W2092206838 @default.
• W4313203764 cites W2099540110 @default.
• W4313203764 cites W2116529502 @default.
• W4313203764 cites W2116593005 @default.
• W4313203764 cites W2135360752 @default.
• W4313203764 cites W2136859480 @default.
• W4313203764 cites W2140176615 @default.
• W4313203764 cites W2154246824 @default.
• W4313203764 cites W2155524192 @default.
• W4313203764 cites W2156703546 @default.
• W4313203764 cites W2264554850 @default.
• W4313203764 cites W2406685744 @default.
• W4313203764 cites W2408954743 @default.
• W4313203764 cites W2550397826 @default.
• W4313203764 cites W2558870407 @default.
• W4313203764 cites W2606747134 @default.
• W4313203764 cites W2724290332 @default.
• W4313203764 cites W2768329455 @default.
• W4313203764 cites W2802977343 @default.
• W4313203764 cites W2889598876 @default.
• W4313203764 cites W2913899896 @default.
• W4313203764 cites W2982787986 @default.
• W4313203764 cites W2992119621 @default.
• W4313203764 cites W2996371967 @default.
• W4313203764 cites W3087351650 @default.
• W4313203764 cites W3092025396 @default.
• W4313203764 cites W3095773942 @default.
• W4313203764 cites W3096321011 @default.
• W4313203764 cites W3111819139 @default.
• W4313203764 cites W3127978946 @default.
• W4313203764 cites W3137719566 @default.
• W4313203764 cites W3157856266 @default.
• W4313203764 cites W3179513642 @default.
• W4313203764 cites W3202497840 @default.
• W4313203764 cites W3203027236 @default.
• W4313203764 cites W3213346990 @default.
• W4313203764 cites W4200174905 @default.
• W4313203764 cites W4213446588 @default.
• W4313203764 cites W4220945603 @default.
• W4313203764 cites W4223963773 @default.
• W4313203764 cites W4224240357 @default.
• W4313203764 cites W4255924814 @default.
• W4313203764 cites W4285214077 @default.
• W4313203764 cites W96437668 @default.
• W4313203764 doi "https://doi.org/10.1371/journal.pone.0279551" @default.
• W4313203764 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36576940" @default.
• W4313203764 hasPublicationYear "2022" @default.
• W4313203764 type Work @default.
• W4313203764 citedByCount "0" @default.
• W4313203764 crossrefType "journal-article" @default.
• W4313203764 hasAuthorship W4313203764A5005630190 @default.
• W4313203764 hasAuthorship W4313203764A5037799592 @default.
• W4313203764 hasAuthorship W4313203764A5075216909 @default.
• W4313203764 hasAuthorship W4313203764A5086969446 @default.
• W4313203764 hasBestOaLocation W43132037641 @default.
• W4313203764 hasConcept C105702510 @default.
• W4313203764 hasConcept C154113507 @default.
• W4313203764 hasConcept C159399396 @default.
• W4313203764 hasConcept C16685009 @default.
• W4313203764 hasConcept C185592680 @default.
• W4313203764 hasConcept C190283241 @default.
• W4313203764 hasConcept C196795494 @default.
• W4313203764 hasConcept C196843134 @default.
• W4313203764 hasConcept C202751555 @default.
• W4313203764 hasConcept C2775966511 @default.
• W4313203764 hasConcept C2778177303 @default.
• W4313203764 hasConcept C512716672 @default.
• W4313203764 hasConcept C55493867 @default.
• W4313203764 hasConcept C71924100 @default.
• W4313203764 hasConcept C86803240 @default.
• W4313203764 hasConcept C87073359 @default.
• W4313203764 hasConcept C88972607 @default.
• W4313203764 hasConcept C95444343 @default.
• W4313203764 hasConceptScore W4313203764C105702510 @default.
• W4313203764 hasConceptScore W4313203764C154113507 @default.
• W4313203764 hasConceptScore W4313203764C159399396 @default.
• W4313203764 hasConceptScore W4313203764C16685009 @default.
• W4313203764 hasConceptScore W4313203764C185592680 @default.
• W4313203764 hasConceptScore W4313203764C190283241 @default.
• W4313203764 hasConceptScore W4313203764C196795494 @default.

• next