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- W4313204007 abstract "Abstract The evolutionary arms race between phages and bacteria, where bacteria evolve resistance to phages and phages retaliate with resistance-countering mutations, is a major driving force of molecular innovation and genetic diversification. Yet attempting to reproduce such ongoing retaliation dynamics in the lab has been challenging; laboratory coevolution experiments of phage and bacteria are typically performed in well-mixed environments and often lead to rapid stagnation with little genetic variability. Here, co-culturing motile E. coli with the lytic bacteriophage T7 on swimming plates, we observe complex spatiotemporal dynamics with multiple genetically diversifying adaptive cycles. Systematically quantifying over 10,000 resistance-infectivity phenotypes between evolved bacteria and phage isolates, we observe diversification into multiple coexisting ecotypes showing a complex interaction network with both host-range expansion and host-switch tradeoffs. Whole-genome sequencing of these evolved phage and bacterial isolates revealed a rich set of adaptive mutations in multiple genetic pathways including in genes not previously linked with phage-bacteria interactions. Synthetically reconstructing these new mutations, we discover phage-general and phage-specific resistance phenotypes as well as a strong synergy with the more classically known phage-resistance mutations. These results highlight the importance of spatial structure and migration for driving phage-bacteria coevolution, providing a concrete system for revealing new molecular mechanisms across diverse phage-bacterial systems." @default.
- W4313204007 created "2023-01-06" @default.
- W4313204007 creator A5062737173 @default.
- W4313204007 creator A5083187104 @default.
- W4313204007 date "2022-12-28" @default.
- W4313204007 modified "2023-10-13" @default.
- W4313204007 title "Multistep diversification in spatiotemporal bacterial-phage coevolution" @default.
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- W4313204007 doi "https://doi.org/10.1038/s41467-022-35351-w" @default.
- W4313204007 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36577749" @default.
- W4313204007 hasPublicationYear "2022" @default.
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