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• W4313220261 abstract "Kisspeptins are known as neuropeptides encoded by the KISS1 gene, which potentiates GnRH neuron excitability. Unfortunately, the role of the KISS1 gene in reproductive traits remains unclear in Indonesian native goat breeds. The current study purposed to detect the genetic variation of the KISS1 gene and investigate the association with reproductive traits in goats to acquire marker-assisted selection (MAS) for the breeding program. Further, ninety blood samples from randomly selected animals were used for DNA isolation and SNP genotyping. The blood serum was collected from sixteen treated goat does to assess FSH levels using ELISA method. The differences between studied parameters were determined using independent samples T-test from SAS Software. Two SNPs, g.2055A&gt;C (SNP1) and g.2064T&gt;A (SNP2) were discovered in intron 1 of KISS1 gene in Indonesian native goat breeds. Two genotypes were identified from each SNPs: AA and AC at SNP1 and TT and TA at SNP2. The allele A (0.96) at SNP1 and allele T (0.93) at SNP2 were discovered as dominant alleles in the population. Furthermore, chi-square test showed that both SNPs were under Hardy-Weinberg equilibrium. The variants of KISS1 gene have no effect on litter size (p&gt;0.05). Moreover, AA genotype of SNP1 had a higher FSH level than that observed in AC genotype (p&lt;0.0001), especially in the follicular phase. SNP2 does not correlate with FSH level relatively (p=0.23). Furthermore, using of AA genotype at SNP1 of KISS1 gene as MAS for the breeding selection program could escalate the reproductive traits in goats" @default.
• W4313220261 created "2023-01-06" @default.
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• W4313220261 date "2022-12-30" @default.
• W4313220261 modified "2023-10-14" @default.
• W4313220261 title "Novel g.2055A>C and g.2064T>A Polymorphisms of KISS1 Gene and Its Association with Reproductive Traits in Local Indonesian Goats" @default.
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• W4313220261 doi "https://doi.org/10.29133/yyutbd.1136214" @default.
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