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• W4313225281 endingPage "505" @default.
• W4313225281 startingPage "505" @default.
• W4313225281 abstract "To study mechanisms involved in fertility, many experimental assays are conducted by incubating spermatozoa in the presence of molecules dissolved in solvents such as ethanol (EtOH) or dimethyl sulfoxide (DMSO). Although a vehicle control group is usually included in such studies, it does not allow to evaluate the intrinsic effect of the solvent on sperm parameters and its potential influence on the outcome of the experiment. In the present study, we incubated human spermatozoa for 4 h in a capacitation medium in the absence or the presence of different concentrations of EtOH and DMSO (0.1, 0.5, 1.0, and 2.0%) to assess the impact of these solvents on sperm motility, vitality, capacitation, and acrosome integrity. The presence of statistically significant relationships between increasing solvent concentrations and the investigated parameters was assessed using linear mixed models. A significant effect was observed with both solvents for total and progressive sperm motilities. We also evaluated the effect of time for these parameters and showed that the influence of the solvents was stable between 0 and 4 h, indicating an almost direct impact of the solvents. While EtOH did not influence sperm vitality and acrosome integrity, a significant effect of increasing DMSO concentrations was observed for these parameters. Finally, regarding capacitation, measured via phosphotyrosine content, although a dose-dependent effect was observed with both solvents, the statistical analysis did not allow to precisely evaluate the intensity of the effect. Based on the results obtained in the present study, and the corresponding linear mixed models, we calculated the concentration of both solvents which would result in a 5% decline in sperm parameters. For EtOH, these concentrations are 0.9, 0.7, and 0.3% for total motility, progressive motility, and capacitation, respectively, while for DMSO they are 1.5, 1.1, >2, 0.3 and >2% for total motility, progressive motility, vitality, capacitation, and acrosome integrity, respectively. We recommend using solvent concentrations below these values to dissolve molecules used to study sperm function in vitro, to limit side effects." @default.
• W4313225281 created "2023-01-06" @default.
• W4313225281 creator A5003824022 @default.
• W4313225281 creator A5004217839 @default.
• W4313225281 creator A5020250761 @default.
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• W4313225281 creator A5080618593 @default.
• W4313225281 date "2022-12-28" @default.
• W4313225281 modified "2023-10-14" @default.
• W4313225281 title "Influence of Two Widely Used Solvents, Ethanol and Dimethyl Sulfoxide, on Human Sperm Parameters" @default.
• W4313225281 cites W1485981369 @default.
• W4313225281 cites W1493078618 @default.
• W4313225281 cites W1529032236 @default.
• W4313225281 cites W1818971580 @default.
• W4313225281 cites W1920065221 @default.
• W4313225281 cites W1951180633 @default.
• W4313225281 cites W1972656114 @default.
• W4313225281 cites W1978559815 @default.
• W4313225281 cites W1983180075 @default.
• W4313225281 cites W1988023503 @default.
• W4313225281 cites W1992894185 @default.
• W4313225281 cites W1998599561 @default.
• W4313225281 cites W2008677281 @default.
• W4313225281 cites W2021164331 @default.
• W4313225281 cites W2025353676 @default.
• W4313225281 cites W2030244780 @default.
• W4313225281 cites W2031904808 @default.
• W4313225281 cites W2034751122 @default.
• W4313225281 cites W2049700436 @default.
• W4313225281 cites W2050601207 @default.
• W4313225281 cites W2055703635 @default.
• W4313225281 cites W2059421386 @default.
• W4313225281 cites W2063181241 @default.
• W4313225281 cites W2064265703 @default.
• W4313225281 cites W2067894224 @default.
• W4313225281 cites W2071053128 @default.
• W4313225281 cites W2072596489 @default.
• W4313225281 cites W2073673423 @default.
• W4313225281 cites W2092505280 @default.
• W4313225281 cites W2095051171 @default.
• W4313225281 cites W2096881309 @default.
• W4313225281 cites W2101472905 @default.
• W4313225281 cites W2106521999 @default.
• W4313225281 cites W2109121284 @default.
• W4313225281 cites W2122542121 @default.
• W4313225281 cites W2124666034 @default.
• W4313225281 cites W2128233254 @default.
• W4313225281 cites W2133848043 @default.
• W4313225281 cites W2140192221 @default.
• W4313225281 cites W2151523282 @default.
• W4313225281 cites W2163737391 @default.
• W4313225281 cites W2164724339 @default.
• W4313225281 cites W2168514659 @default.
• W4313225281 cites W2175091752 @default.
• W4313225281 cites W2254256350 @default.
• W4313225281 cites W2265017625 @default.
• W4313225281 cites W2280753520 @default.
• W4313225281 cites W2332820183 @default.
• W4313225281 cites W2401326686 @default.
• W4313225281 cites W2464550160 @default.
• W4313225281 cites W2474931230 @default.
• W4313225281 cites W2525362313 @default.
• W4313225281 cites W2560157912 @default.
• W4313225281 cites W2750174518 @default.
• W4313225281 cites W2750248999 @default.
• W4313225281 cites W2754624447 @default.
• W4313225281 cites W2908852231 @default.
• W4313225281 cites W2921406196 @default.
• W4313225281 cites W2980901582 @default.
• W4313225281 cites W3000506953 @default.
• W4313225281 cites W3002548424 @default.
• W4313225281 cites W3004389986 @default.
• W4313225281 cites W3007181795 @default.
• W4313225281 cites W3040674829 @default.
• W4313225281 cites W3042547560 @default.
• W4313225281 cites W3043992529 @default.
• W4313225281 cites W3109057831 @default.
• W4313225281 cites W3122389709 @default.
• W4313225281 cites W3125670692 @default.
• W4313225281 cites W3126385987 @default.
• W4313225281 cites W3133896934 @default.
• W4313225281 cites W3160566299 @default.
• W4313225281 cites W3210283221 @default.
• W4313225281 cites W3211331997 @default.
• W4313225281 cites W4238550077 @default.
• W4313225281 cites W4241261134 @default.
• W4313225281 cites W4294193011 @default.
• W4313225281 cites W2048935070 @default.
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• W4313225281 doi "https://doi.org/10.3390/ijms24010505" @default.
• W4313225281 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36613946" @default.
• W4313225281 hasPublicationYear "2022" @default.
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