FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313237026> ?p ?o ?g. }

• W4313237026 endingPage "1114" @default.
• W4313237026 startingPage "1103" @default.
• W4313237026 abstract "The genomic 5'-terminal regions of viruses in the family Potyviridae (potyvirids) encode two types of leader proteases: serine-protease (P1) and cysteine-protease (HCPro), which differ greatly in the arrangement and sequence composition among inter-genus viruses. Most potyvirids have the same tandemly arranged P1 and HCPro, whereas viruses in the genus Macluravirus encode a single distinct leader protease, a truncated version of HCPro with yet-unknown functions. We investigated the RNA silencing suppression (RSS) activity and its underpinning mechanism of the distinct HCPro from alpinia oxyphylla mosaic macluravirus (aHCPro). Sequence analysis revealed that macluraviral HCPros have obvious truncations in the N-terminal and middle regions when aligned to their counterparts in potyviruses (well-characterized viral suppressors of RNA silencing). Nearly all defined elements essential for the RSS activity of potyviral counterparts are not distinguished in macluraviral HCPros. Here, we demonstrated that aHCPro exhibits a similar anti-silencing activity with the potyviral counterpart. However, aHCPro fails to block both the local and systemic spreading of RNA silencing. In line, aHCPro interferes with the dsRNA synthesis, an upstream step in the RNA silencing pathway. Affinity-purification and NanoLC-MS/MS analysis revealed that aHCPro has no association with core components or their potential interactors involving in dsRNA synthesis from the protein layer. Instead, the ectopic expression of aHCPro significantly reduces the transcript abundance of RDR2, RDR6, SGS3, and SDE5. This study represents the first report on the anti-silencing function of Macluravirus-encoded HCPro and the underlying molecular mechanism." @default.
• W4313237026 created "2023-01-06" @default.
• W4313237026 creator A5005868099 @default.
• W4313237026 creator A5013169889 @default.
• W4313237026 creator A5022633553 @default.
• W4313237026 creator A5059118083 @default.
• W4313237026 creator A5062028860 @default.
• W4313237026 creator A5072679711 @default.
• W4313237026 creator A5085467224 @default.
• W4313237026 date "2023-06-01" @default.
• W4313237026 modified "2023-10-15" @default.
• W4313237026 title "The Single Distinct Leader Protease Encoded by Alpinia oxyphylla Mosaic Virus (Genus <i>Macluravirus</i>) Suppresses RNA Silencing Through Interfering with Double-Stranded RNA Synthesis" @default.
• W4313237026 cites W1484130581 @default.
• W4313237026 cites W1571838327 @default.
• W4313237026 cites W1631037284 @default.
• W4313237026 cites W1926826741 @default.
• W4313237026 cites W1963759242 @default.
• W4313237026 cites W1971373251 @default.
• W4313237026 cites W1978439509 @default.
• W4313237026 cites W1979965542 @default.
• W4313237026 cites W1986173894 @default.
• W4313237026 cites W1989174148 @default.
• W4313237026 cites W1990140893 @default.
• W4313237026 cites W1990301918 @default.
• W4313237026 cites W1995242378 @default.
• W4313237026 cites W2004764824 @default.
• W4313237026 cites W2018232804 @default.
• W4313237026 cites W2034144108 @default.
• W4313237026 cites W2048514397 @default.
• W4313237026 cites W2051219024 @default.
• W4313237026 cites W2054762956 @default.
• W4313237026 cites W2057132428 @default.
• W4313237026 cites W2061353802 @default.
• W4313237026 cites W2062188301 @default.
• W4313237026 cites W2064908295 @default.
• W4313237026 cites W2069406055 @default.
• W4313237026 cites W2069507179 @default.
• W4313237026 cites W2070178104 @default.
• W4313237026 cites W2085916168 @default.
• W4313237026 cites W2087123132 @default.
• W4313237026 cites W2092002119 @default.
• W4313237026 cites W2097788615 @default.
• W4313237026 cites W2098135714 @default.
• W4313237026 cites W2098500177 @default.
• W4313237026 cites W2099308244 @default.
• W4313237026 cites W2100933194 @default.
• W4313237026 cites W2101172036 @default.
• W4313237026 cites W2106215558 @default.
• W4313237026 cites W2107330252 @default.
• W4313237026 cites W2109590074 @default.
• W4313237026 cites W2110403330 @default.
• W4313237026 cites W2111092051 @default.
• W4313237026 cites W2114661854 @default.
• W4313237026 cites W2118196659 @default.
• W4313237026 cites W2128393675 @default.
• W4313237026 cites W2129401531 @default.
• W4313237026 cites W2135916248 @default.
• W4313237026 cites W2151286394 @default.
• W4313237026 cites W2153327762 @default.
• W4313237026 cites W2159675211 @default.
• W4313237026 cites W2168040612 @default.
• W4313237026 cites W2191033864 @default.
• W4313237026 cites W2265474754 @default.
• W4313237026 cites W2284373438 @default.
• W4313237026 cites W2343639134 @default.
• W4313237026 cites W2537623931 @default.
• W4313237026 cites W2599029328 @default.
• W4313237026 cites W2605265667 @default.
• W4313237026 cites W2769524157 @default.
• W4313237026 cites W2803601536 @default.
• W4313237026 cites W2888121167 @default.
• W4313237026 cites W2895829909 @default.
• W4313237026 cites W2897138677 @default.
• W4313237026 cites W2951172563 @default.
• W4313237026 cites W2952411272 @default.
• W4313237026 cites W2957622437 @default.
• W4313237026 cites W2981389845 @default.
• W4313237026 cites W3040064942 @default.
• W4313237026 cites W3080338516 @default.
• W4313237026 cites W3089774170 @default.
• W4313237026 cites W3092088984 @default.
• W4313237026 cites W3152851559 @default.
• W4313237026 cites W3193439546 @default.
• W4313237026 cites W3199801754 @default.
• W4313237026 cites W4200335466 @default.
• W4313237026 cites W4210884586 @default.
• W4313237026 cites W4213073514 @default.
• W4313237026 cites W4221060531 @default.
• W4313237026 cites W4225408347 @default.
• W4313237026 cites W4234681447 @default.
• W4313237026 cites W4281763697 @default.
• W4313237026 cites W4282601803 @default.
• W4313237026 cites W4282942782 @default.
• W4313237026 cites W4283014056 @default.
• W4313237026 cites W4289525572 @default.
• W4313237026 doi "https://doi.org/10.1094/phyto-10-22-0371-r" @default.
• W4313237026 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36576401" @default.
• W4313237026 hasPublicationYear "2023" @default.

• next