Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313237046> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W4313237046 endingPage "1404" @default.
- W4313237046 startingPage "1393" @default.
- W4313237046 abstract "Systemic RNA interference deficient-1-like (SIL1) is considered a core component in dsRNA uptake in some insect species. Investigation related to the potential function of SIL1 in dsRNA uptake can contribute to a further understanding of RNA interference (RNAi) mechanisms in insects and agricultural pest control. However, the role of SIL1 in dsRNA uptake in insects such as aphids remains controversial. We have thoroughly analyzed the role of SIL1 from the model aphid Acyrthosiphon pisum (ApSIL1) in cellular dsRNA to clarify its function. First, the induced expression of ApSIL1 upon dsRNA oral exposure provided a vital clue for the possible involvement of ApSIL1 in cellular dsRNA uptake. Subsequent in vivo experiments using the RNAi-of-RNAi approach for ApSIL1 supported our hypothesis that the silencing efficiencies of reporter genes were reduced after inhibition of ApSIL1 expression. The impaired biological phenotypes of aphids, including cumulative average offspring, deformities of the nymph, and mortality upon pathogen infection, were then observed in the treatment group. Thereafter, in vitro dual-luciferase reporter assay showed compelling evidence that the luciferin signal was significantly attenuated when dsluciferase or dsGFP was transferred into ApSIL1-transfected Drosophila S2 cells. These observations further confirmed that the signal of Cy3-labeled dsRNA was rapidly attenuated with time in ApSIL1-transfected Drosophila S2 cells. Overall, these findings conclusively establish that ApSIL1 is involved in dsRNA uptake in A. pisum." @default.
- W4313237046 created "2023-01-06" @default.
- W4313237046 creator A5015321937 @default.
- W4313237046 creator A5035130242 @default.
- W4313237046 creator A5040924648 @default.
- W4313237046 creator A5060352086 @default.
- W4313237046 creator A5071983422 @default.
- W4313237046 creator A5089443813 @default.
- W4313237046 date "2023-01-20" @default.
- W4313237046 modified "2023-10-17" @default.
- W4313237046 title "The involvement of systemic RNA interference deficient‐1‐like (SIL1) in cellular dsRNA uptake in <i>Acyrthosiphon pisum</i>" @default.
- W4313237046 cites W1517658796 @default.
- W4313237046 cites W1900843105 @default.
- W4313237046 cites W1933947420 @default.
- W4313237046 cites W1998502303 @default.
- W4313237046 cites W2026599884 @default.
- W4313237046 cites W2038312319 @default.
- W4313237046 cites W2038388342 @default.
- W4313237046 cites W2077293902 @default.
- W4313237046 cites W2079902244 @default.
- W4313237046 cites W2095870428 @default.
- W4313237046 cites W2101728241 @default.
- W4313237046 cites W2124791602 @default.
- W4313237046 cites W2140260047 @default.
- W4313237046 cites W2148264517 @default.
- W4313237046 cites W2270468432 @default.
- W4313237046 cites W2294164036 @default.
- W4313237046 cites W2604845045 @default.
- W4313237046 cites W2757754088 @default.
- W4313237046 cites W2762893336 @default.
- W4313237046 cites W2885524011 @default.
- W4313237046 cites W2910734159 @default.
- W4313237046 cites W3047925960 @default.
- W4313237046 cites W3094019675 @default.
- W4313237046 cites W3111034880 @default.
- W4313237046 cites W3133093595 @default.
- W4313237046 cites W3165015570 @default.
- W4313237046 doi "https://doi.org/10.1111/1744-7917.13167" @default.
- W4313237046 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36576078" @default.
- W4313237046 hasPublicationYear "2023" @default.
- W4313237046 type Work @default.
- W4313237046 citedByCount "1" @default.
- W4313237046 crossrefType "journal-article" @default.
- W4313237046 hasAuthorship W4313237046A5015321937 @default.
- W4313237046 hasAuthorship W4313237046A5035130242 @default.
- W4313237046 hasAuthorship W4313237046A5040924648 @default.
- W4313237046 hasAuthorship W4313237046A5060352086 @default.
- W4313237046 hasAuthorship W4313237046A5071983422 @default.
- W4313237046 hasAuthorship W4313237046A5089443813 @default.
- W4313237046 hasConcept C104317684 @default.
- W4313237046 hasConcept C119056186 @default.
- W4313237046 hasConcept C166703698 @default.
- W4313237046 hasConcept C2191507 @default.
- W4313237046 hasConcept C22508944 @default.
- W4313237046 hasConcept C2776385806 @default.
- W4313237046 hasConcept C2776988988 @default.
- W4313237046 hasConcept C2781350953 @default.
- W4313237046 hasConcept C54009773 @default.
- W4313237046 hasConcept C54355233 @default.
- W4313237046 hasConcept C59822182 @default.
- W4313237046 hasConcept C67705224 @default.
- W4313237046 hasConcept C86803240 @default.
- W4313237046 hasConcept C95444343 @default.
- W4313237046 hasConceptScore W4313237046C104317684 @default.
- W4313237046 hasConceptScore W4313237046C119056186 @default.
- W4313237046 hasConceptScore W4313237046C166703698 @default.
- W4313237046 hasConceptScore W4313237046C2191507 @default.
- W4313237046 hasConceptScore W4313237046C22508944 @default.
- W4313237046 hasConceptScore W4313237046C2776385806 @default.
- W4313237046 hasConceptScore W4313237046C2776988988 @default.
- W4313237046 hasConceptScore W4313237046C2781350953 @default.
- W4313237046 hasConceptScore W4313237046C54009773 @default.
- W4313237046 hasConceptScore W4313237046C54355233 @default.
- W4313237046 hasConceptScore W4313237046C59822182 @default.
- W4313237046 hasConceptScore W4313237046C67705224 @default.
- W4313237046 hasConceptScore W4313237046C86803240 @default.
- W4313237046 hasConceptScore W4313237046C95444343 @default.
- W4313237046 hasIssue "5" @default.
- W4313237046 hasLocation W43132370461 @default.
- W4313237046 hasLocation W43132370462 @default.
- W4313237046 hasOpenAccess W4313237046 @default.
- W4313237046 hasPrimaryLocation W43132370461 @default.
- W4313237046 hasRelatedWork W153428622 @default.
- W4313237046 hasRelatedWork W2073840770 @default.
- W4313237046 hasRelatedWork W2079887141 @default.
- W4313237046 hasRelatedWork W2100394661 @default.
- W4313237046 hasRelatedWork W2884140292 @default.
- W4313237046 hasRelatedWork W2910734159 @default.
- W4313237046 hasRelatedWork W2981114495 @default.
- W4313237046 hasRelatedWork W3140077495 @default.
- W4313237046 hasRelatedWork W3144044208 @default.
- W4313237046 hasRelatedWork W4295095065 @default.
- W4313237046 hasVolume "30" @default.
- W4313237046 isParatext "false" @default.
- W4313237046 isRetracted "false" @default.
- W4313237046 workType "article" @default.