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- W4313237480 abstract "To observe the effect of moxibustion on Janus protein tyrosine kinase 2/signal transducer and activator of transcription 3 (JAK2-STAT3) signal pathway and interleukin (IL)-1β and IL-18 in synovial fluid of adjuvant arthritis (AA) rabbits, so as to explore the mechanism of moxibustion in the treatment of rheumatoid arthritis.Twenty-eight rabbits were randomized into control, model, moxibustion, and NLRP3 overexpression groups, with 7 rabbits in each group. AA rabbit model was established by injection of Freund's complete adjuvant (FCA, 0.5 mL/kg) into the rabbits' bilateral hind-knee joint cavities. On the third day after modeling, the NLRP3 lentiviral vector (40 μL) were injected into the bilateral hind-knee joint cavities of rabbits in NLRP3 overexpression group. Moxibustion was used to bilateral Shenshu (BL23) and Zusanli (ST36), 5 cones every time, once daily, 6 days a week for 3 weeks in the moxibustion and NLRP3 overexpression groups. The perimeters of rabbits' hind legs were measured after modeling and after the intervention. The contents of IL-1β, IL-18 in synovial fluid were detected by ELISA and the expression levels of NLRP3, JAK2 and STAT3 mRNAs in synovial tissue were detected by real-time PCR.In comparison with the control group, the perimeters of bilateral knee joints were significantly increased at each time point (P<0.05),and the contents of IL-1β, IL-18 in synovial fluid and the expression levels of NLRP3, JAK2, STAT3 mRNA in synovial tissue were significantly increased (P<0.05) in the model group. Compared with the model group, the perimeters of bilateral knee joints were significantly decreased (P<0.05), and the contents of IL-1β, IL-18 in synovial fluid and the expression levels of NLRP3, JAK2, STAT3 mRNAs in synovial tissue were significantly decreased (P<0.05) in the moxibusion group. Compared with the moxibustion group, the above indexes were higher in the NLRP3 overexpression group (P<0.05).Moxibustion may play an anti-inflammatory and detumescent role in AA rabbits by regulating JAK2-STAT3 signal pathway, and its therapeutic effect may be closely related to the expression of NLRP3.目的：通过慢病毒载体介导NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎性小体在佐剂性关节炎(AA)家兔中过表达，观察艾灸对AA家兔Janus蛋白酪氨酸激酶2-信号转导和转录激活因子3(JAK2-STAT3)信号通路及滑膜液白细胞介素（IL)-1β、IL-18的影响，探讨艾灸治疗类风湿性关节炎的可能机制。方法：将28只家兔随机分为对照组、模型组、艾灸组、NLRP3过表达组，每组7只。采用弗氏完全佐剂注射法制备AA家兔模型。造模后第3天，将NLRP3过表达慢病毒载体注入NLRP3过表达组家兔双侧后膝关节腔内。造模后第7天，艾灸组、NLRP3过表达组给予艾炷灸双侧“肾俞”“足三里”治疗，5壮/穴，1次/d,6 d为1个疗程，疗程间间隔1 d，共3个疗程。造模后第6天、治疗第6天及治疗结束后测量家兔双后膝关节周长。采用ELISA法检测家兔膝关节滑膜液中IL-1β、IL-18的含量，实时荧光定量PCR法检测家兔膝关节滑膜组织中NLRP3、JAK2、STAT3 mRNA表达。结果：与对照组比较，模型组家兔左、右膝关节周长及膝关节滑膜液中IL-1β、IL-18含量增加(P<0.05)，滑膜组织中NLRP3、JAK2、STAT3 mRNA表达均升高(P<0.05)；与模型组比较，艾灸组左、右膝关节周长缩小(P<0.05)，膝关节滑膜液中IL-1β、IL-18含量及膝关节滑膜组织中NLRP3、JAK2、STAT3 mRNA表达均降低(P<0.05)；与艾灸组比较，NLRP3过表达组左、右膝关节周长及膝关节滑膜液中IL-1β、IL-18含量增加(P<0.05)，膝关节滑膜组织中NLRP3、JAK2、STAT3 mRNA表达均升高(P<0.05)。结论：艾灸可能通过调控JAK2-STAT3信号通路发挥对AA家兔的抗炎消肿作用，其治疗效果可能与NLRP3表达密切相关。." @default.
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- W4313237480 date "2022-12-25" @default.
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- W4313237480 title "[Effect of moxibustion on JAK2/STAT3 signal pathway in synovial tissues of rabbits with adjuvant arthritis under the overexpression of NLRP3 inflammatory bodies]." @default.
- W4313237480 doi "https://doi.org/10.13702/j.1000-0607.20211160" @default.
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