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- W4313238878 abstract "The denaturing gradient gel electrophoresis (DGGE) is an effective culture-independent technique for assessing diversity of microbial communities. In this study, the PCR-DGGE technique was applied for analyzing the diversity of bacterial communities in marine sediment in the Truong Sa archipelago (Spratly Islands), Vietnam, and a comparison with cultivable bacterial fraction was performed. Three sediment samples taken from different depths were used in the study. A total of 17 bacterial strains were isolated and divided into 6 groups based on their colony and cell morphologies. Comparative analyses of nearly full-length 16S rDNA sequences retrieved from six representative strains of these morphologically distinguished groups indicated that they were most closely related to Bacillus amyloliquefaciens, Bacillus filamentosus, Bacillus endophyticus, Bacillus licheniformis, Pseudomonas balearica, and Raoultella ornithinolytica. The PCR-DGGE analyses carried out with the three sediment samples showed relatively diverse bacterial communities with 9 distinct bands, 6 of which were related to the isolated representatives, whereas the other three were identified as Vibrio proteolyticus, Priestia filamentosa, and Clostridium halophilum. Thus, by applying the PCR-DGGE technique, more thorough information on bacterial communities in the sediment of Truong Sa archipelago could be elucidated, providing useful information for further studies on the conservation and utilization of microbial resources in this ecosystem." @default.
- W4313238878 created "2023-01-06" @default.
- W4313238878 creator A5044797288 @default.
- W4313238878 date "2021-01-01" @default.
- W4313238878 modified "2023-10-07" @default.
- W4313238878 title "Nghiên cứu đa dạng vi khuẩn trong trầm tích biển tại quần đảo Trường Sa bằng phương pháp truyền thống kết hợp kỹ thuật điện di biến tính" @default.
- W4313238878 doi "https://doi.org/10.58334/vrtc.jtst.n24.14" @default.
- W4313238878 hasPublicationYear "2021" @default.
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