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- W4313238894 abstract "Yeast (Candida albicans) is the term for the fungus that exists as a single celled organism rather than as hyphae. In the present study Methyl chavicol a major constituent of Holy Basil (Ocimum sanctum) essential oil has been evaluated against the induction of germ tubes in Candida albicans. Ocimum Sanctum leaves and seeds were used for extraction of basil oil through stream distillation process. Candida cells were also grown in yeast extract, peptone and dextrose (1:2:2) medium. Primary culture (0.5%) of Candida albicans was mixed with 25ml of Lee’s modified media which was grown for 72 h at 25oC and was centrifuged at 5000 rpm for 5 min. The cells obtained in the form of pellet were washed with distilled water and then reinoculated into fresh 50ml LMM and the test compound was added. pH of the solution was adjusted to 6.5 at 37oC by adding 0.1 N NaOH solution. Then slides were prepared after every 90 min interval for 24 h and observed under inverted microscope. After 90 min in all the three sets, germ tube induction begins but the length of the germ tube greatly differs. In the control the maximum length observed is 38.3 μm but in MC1 and MC2 it is 9.4μm and 5.7μm, respectively. This clearly shows the inhibitory effects of methyl chavicol on germ tube induction. Similar results of decreasing length of germ tubes in MC2 at different time periods, proved that MC2 i.e. 70μg/mL methyl chavicol is a potent inhibitor of germ tubes induction." @default.
- W4313238894 created "2023-01-06" @default.
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- W4313238894 date "2015-12-09" @default.
- W4313238894 modified "2023-10-12" @default.
- W4313238894 title "The in vivo effect of Methyl Chavicol, on the Candida albicans Germ Tube Induction" @default.
- W4313238894 doi "https://doi.org/10.57233/ijsgs.v1i1.305" @default.
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