Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313246911> ?p ?o ?g. }
- W4313246911 endingPage "72" @default.
- W4313246911 startingPage "72" @default.
- W4313246911 abstract "In phagocytes, cytoskeletal and membrane remodeling is finely regulated at the phagocytic cup. Various smaFll G proteins, including those of the Arf family, control these dynamic processes. Human neutrophils express AGAP2, an Arf GTPase activating protein (ArfGAP) that regulates endosomal trafficking and focal adhesion remodeling. We first examined the impact of AGAP2 on phagocytosis in CHO cells stably expressing the FcγRIIA receptor (CHO-IIA). In unstimulated CHO-IIA cells, AGAP2 only partially co-localized with cytoskeletal elements and intracellular compartments. In CHO-IIA cells, AGAP2 transiently accumulated at actin-rich phagocytic cups and increased Fcγ receptor-mediated phagocytosis. Enhanced phagocytosis was not dependent on the N-terminal GTP-binding protein-like (GLD) domain of AGAP2. AGAP2 deleted of its GTPase-activating protein (GAP) domain was not recruited to phagocytic cups and did not enhance the engulfment of IgG-opsonized beads. However, the GAP-deficient [R618K]AGAP2 transiently localized at the phagocytic cups and enhanced phagocytosis. In PLB-985 cells differentiated towards a neutrophil-like phenotype, silencing of AGAP2 reduced phagocytosis of opsonized zymosan. In human neutrophils, opsonized zymosan or monosodium urate crystals induced AGAP2 phosphorylation. The data indicate that particulate agonists induce AGAP2 phosphorylation in neutrophils. This study highlights the role of AGAP2 and its GAP domain but not GAP activity in FcγR-dependent uptake of opsonized particles." @default.
- W4313246911 created "2023-01-06" @default.
- W4313246911 creator A5030871124 @default.
- W4313246911 creator A5042032791 @default.
- W4313246911 creator A5063674894 @default.
- W4313246911 creator A5085204114 @default.
- W4313246911 date "2022-12-24" @default.
- W4313246911 modified "2023-09-27" @default.
- W4313246911 title "Functional Role of AGAP2/PIKE-A in Fcγ Receptor-Mediated Phagocytosis" @default.
- W4313246911 cites W147011734 @default.
- W4313246911 cites W1494029578 @default.
- W4313246911 cites W1498039314 @default.
- W4313246911 cites W1523536630 @default.
- W4313246911 cites W1564846750 @default.
- W4313246911 cites W1705205990 @default.
- W4313246911 cites W1819249925 @default.
- W4313246911 cites W1901220203 @default.
- W4313246911 cites W1927734345 @default.
- W4313246911 cites W1945008950 @default.
- W4313246911 cites W1968337792 @default.
- W4313246911 cites W1975417922 @default.
- W4313246911 cites W1975750284 @default.
- W4313246911 cites W1975854882 @default.
- W4313246911 cites W1977966822 @default.
- W4313246911 cites W1978574720 @default.
- W4313246911 cites W1979742234 @default.
- W4313246911 cites W1983723496 @default.
- W4313246911 cites W1984474514 @default.
- W4313246911 cites W1986296729 @default.
- W4313246911 cites W1988243126 @default.
- W4313246911 cites W1996500612 @default.
- W4313246911 cites W1997910541 @default.
- W4313246911 cites W1999096447 @default.
- W4313246911 cites W2007744273 @default.
- W4313246911 cites W2011657093 @default.
- W4313246911 cites W2014495816 @default.
- W4313246911 cites W2022322187 @default.
- W4313246911 cites W2026370041 @default.
- W4313246911 cites W2028273808 @default.
- W4313246911 cites W2040940476 @default.
- W4313246911 cites W2046523890 @default.
- W4313246911 cites W2049638147 @default.
- W4313246911 cites W2049804931 @default.
- W4313246911 cites W2055519551 @default.
- W4313246911 cites W2061171898 @default.
- W4313246911 cites W2066565605 @default.
- W4313246911 cites W2071078803 @default.
- W4313246911 cites W2071421185 @default.
- W4313246911 cites W2073794206 @default.
- W4313246911 cites W2073897763 @default.
- W4313246911 cites W2075631488 @default.
- W4313246911 cites W2076431194 @default.
- W4313246911 cites W2079261541 @default.
- W4313246911 cites W2084289465 @default.
- W4313246911 cites W2085009435 @default.
- W4313246911 cites W2085213378 @default.
- W4313246911 cites W2086012892 @default.
- W4313246911 cites W2086513049 @default.
- W4313246911 cites W2088618181 @default.
- W4313246911 cites W2092986430 @default.
- W4313246911 cites W2093844155 @default.
- W4313246911 cites W2096716574 @default.
- W4313246911 cites W2097416981 @default.
- W4313246911 cites W2098436412 @default.
- W4313246911 cites W2106848201 @default.
- W4313246911 cites W2108594642 @default.
- W4313246911 cites W2109252703 @default.
- W4313246911 cites W2115637834 @default.
- W4313246911 cites W2119466361 @default.
- W4313246911 cites W2124733101 @default.
- W4313246911 cites W2127432085 @default.
- W4313246911 cites W2127810225 @default.
- W4313246911 cites W2134916100 @default.
- W4313246911 cites W2135266792 @default.
- W4313246911 cites W2135565251 @default.
- W4313246911 cites W2142468313 @default.
- W4313246911 cites W2145355599 @default.
- W4313246911 cites W2147359514 @default.
- W4313246911 cites W2147858442 @default.
- W4313246911 cites W2147950475 @default.
- W4313246911 cites W2149302237 @default.
- W4313246911 cites W2151547335 @default.
- W4313246911 cites W2152275257 @default.
- W4313246911 cites W2152511989 @default.
- W4313246911 cites W2156723896 @default.
- W4313246911 cites W2157463926 @default.
- W4313246911 cites W2157805702 @default.
- W4313246911 cites W2158645659 @default.
- W4313246911 cites W2165448884 @default.
- W4313246911 cites W2170507686 @default.
- W4313246911 cites W2331822890 @default.
- W4313246911 cites W2751637650 @default.
- W4313246911 cites W2918168669 @default.
- W4313246911 cites W2930125518 @default.
- W4313246911 cites W2950635409 @default.
- W4313246911 cites W2951627794 @default.
- W4313246911 cites W3007396194 @default.
- W4313246911 cites W3150557828 @default.