Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313247146> ?p ?o ?g. }
- W4313247146 endingPage "90" @default.
- W4313247146 startingPage "90" @default.
- W4313247146 abstract "Disrupting bacterial quorum sensing (QS) signaling is a promising strategy to combat pathogenic biofilms without the development of antibiotic resistance. Here, we report that food-associated bacteria can interfere with the biofilm formation of a Gram-negative pathogenic bacterium by targeting its AHL (acyl-homoserine lactone) QS system. This was demonstrated by screening metabolic end-products of different lactobacilli and propionibacteria using Gram-negative and biofilm-forming Chromobacterium violaceum as the QS reporter and our anti-QS microscale screening platform with necessary modifications. The method was optimized in terms of the inoculation technique and the concentrations of D-glucose and L-tryptophan, two key factors controlling the synthesis of violacein, a purple pigment indicating the activation of the QS system in C. violaceum. These improvements resulted in ca. 16-times higher violacein yields and enabled revealing anti-QS effects of Lactobacillus acidophilus, Lentilactobacillus kefiri, Lacticaseibacillus rhamnosus and Propionibacterium freudenreichii, including new cheese-associated strains. Our findings also suggest that acetate and propionate excreted by these species are the main factors that interrupt the QS-mediated signaling and subsequent biofilm growth without affecting the cell viability of the C. violaceum reporter. Thus, the present study reports a revised anti-QS screening method to accurately define new bacteria with an ability to combat pathogens in a safe and sustainable way." @default.
- W4313247146 created "2023-01-06" @default.
- W4313247146 creator A5002108219 @default.
- W4313247146 creator A5004712329 @default.
- W4313247146 creator A5013080582 @default.
- W4313247146 creator A5016403744 @default.
- W4313247146 creator A5024299634 @default.
- W4313247146 creator A5024343004 @default.
- W4313247146 creator A5025616346 @default.
- W4313247146 creator A5048624820 @default.
- W4313247146 creator A5056295071 @default.
- W4313247146 creator A5066819098 @default.
- W4313247146 creator A5072217490 @default.
- W4313247146 creator A5073379520 @default.
- W4313247146 date "2022-12-24" @default.
- W4313247146 modified "2023-10-13" @default.
- W4313247146 title "Food-Grade Bacteria Combat Pathogens by Blocking AHL-Mediated Quorum Sensing and Biofilm Formation" @default.
- W4313247146 cites W146411662 @default.
- W4313247146 cites W1991001144 @default.
- W4313247146 cites W1995803803 @default.
- W4313247146 cites W2003138081 @default.
- W4313247146 cites W2024552482 @default.
- W4313247146 cites W2036978891 @default.
- W4313247146 cites W2046374288 @default.
- W4313247146 cites W2050868941 @default.
- W4313247146 cites W2054138948 @default.
- W4313247146 cites W2084937356 @default.
- W4313247146 cites W2096843137 @default.
- W4313247146 cites W2098894017 @default.
- W4313247146 cites W2108810627 @default.
- W4313247146 cites W2113239826 @default.
- W4313247146 cites W2119406110 @default.
- W4313247146 cites W2126668099 @default.
- W4313247146 cites W2139443641 @default.
- W4313247146 cites W2140411292 @default.
- W4313247146 cites W2144657442 @default.
- W4313247146 cites W2147161211 @default.
- W4313247146 cites W2173349102 @default.
- W4313247146 cites W2174196841 @default.
- W4313247146 cites W2211438151 @default.
- W4313247146 cites W2273241946 @default.
- W4313247146 cites W2274972366 @default.
- W4313247146 cites W2401331974 @default.
- W4313247146 cites W2409858324 @default.
- W4313247146 cites W2477385535 @default.
- W4313247146 cites W2511941815 @default.
- W4313247146 cites W2520432018 @default.
- W4313247146 cites W2558511920 @default.
- W4313247146 cites W2593051571 @default.
- W4313247146 cites W2612623519 @default.
- W4313247146 cites W2736894609 @default.
- W4313247146 cites W2767921398 @default.
- W4313247146 cites W2770810200 @default.
- W4313247146 cites W2793156110 @default.
- W4313247146 cites W2795389370 @default.
- W4313247146 cites W2800407297 @default.
- W4313247146 cites W2800799562 @default.
- W4313247146 cites W2802140503 @default.
- W4313247146 cites W2888999738 @default.
- W4313247146 cites W2891306787 @default.
- W4313247146 cites W2900607856 @default.
- W4313247146 cites W2904066077 @default.
- W4313247146 cites W2914792198 @default.
- W4313247146 cites W2921712263 @default.
- W4313247146 cites W2931314706 @default.
- W4313247146 cites W2940924009 @default.
- W4313247146 cites W2941721288 @default.
- W4313247146 cites W2946081661 @default.
- W4313247146 cites W2947220300 @default.
- W4313247146 cites W2965641882 @default.
- W4313247146 cites W2969431986 @default.
- W4313247146 cites W2977005911 @default.
- W4313247146 cites W2980721239 @default.
- W4313247146 cites W2988178326 @default.
- W4313247146 cites W3000424460 @default.
- W4313247146 cites W3007850773 @default.
- W4313247146 cites W3008557842 @default.
- W4313247146 cites W3012106863 @default.
- W4313247146 cites W3034283669 @default.
- W4313247146 cites W3047600906 @default.
- W4313247146 cites W3081308864 @default.
- W4313247146 cites W3087384602 @default.
- W4313247146 cites W3087844092 @default.
- W4313247146 cites W3091416313 @default.
- W4313247146 cites W3093086543 @default.
- W4313247146 cites W3104344870 @default.
- W4313247146 cites W3107885597 @default.
- W4313247146 cites W3127833979 @default.
- W4313247146 cites W3155093286 @default.
- W4313247146 cites W3161544633 @default.
- W4313247146 cites W3168401786 @default.
- W4313247146 cites W3168917783 @default.
- W4313247146 cites W3181856238 @default.
- W4313247146 cites W4200064544 @default.
- W4313247146 cites W4200172703 @default.
- W4313247146 cites W4206385916 @default.
- W4313247146 cites W4210511772 @default.
- W4313247146 cites W4280590059 @default.