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- W4313247727 abstract "Fluorescence imaging through the second near-infrared window (NIR-II,1000–1700 nm) allows in-depth imaging. However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large FoV (field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 µm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 <italic>Ι</italic><sub>contrast</sub>. To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 <italic>Ι</italic><sub>contrast</sub> to resolve the 100 μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm<sup>3</sup> and collect 750 images within 6 mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-II imaging." @default.
- W4313247727 created "2023-01-06" @default.
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- W4313247727 date "2023-01-01" @default.
- W4313247727 modified "2023-10-10" @default.
- W4313247727 title "Deep learning enhanced NIR-II volumetric imaging of whole mice vasculature" @default.
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- W4313247727 doi "https://doi.org/10.29026/oea.2023.220105" @default.
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