FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313249108> ?p ?o ?g. }

• W4313249108 endingPage "2039" @default.
• W4313249108 startingPage "2030" @default.
• W4313249108 abstract "As a rare disease in children, necrotizing enterocolitis (NEC) leads to high morbidity and mortality. However, its pathophysiology is largely unclear. Transient receptor potential melastatin 7 (TRPM7) is a membrane protein, which plays key roles in the inflammatory response. This study sought to examine the promoting effect of TRPM7 on NEC in children and explore the therapeutic effect of a TRPM7 inhibitor NS8593.First, we detected TRPM7 and NLR family pyrin domain containing 3 (NLRP3) expression and the state of inflammation in children with NEC through quantitative real-time polymerase chain reaction (RT-PCR), Western blot, and enzyme-linked immunosorbent assays. Next, Human intestinal epithelial cell lines were induced to NEC by lipopolysaccharides (LPSs). The level of cytokines and reactive oxygen species (ROS) were tested by RT-PCR and flow cytometry. The TRPM7 mediated calcium flux were determined by fluorescence. In addition, we used the TRPM7 inhibitor NS8593 to treat the in vivo rat model. The mRNA and protein expression were determined by real-time PCR and Elisa analysis, respectively.TRPM7 and NLRP3 expression was more increased in the samples from children with NEC compared to the control samples. Additionally, the elevated secretion of interleukin-1β, interleukin-6, and tumor necrosis factor alpha was also detected in the serum of children with NEC. These results showed that TRPM7 had a promoting effect on NEC development, possibly via the activation of NLRP3. To test our hypothesis, the TRPM7 inhibitor NS8593 was used to treat the LPS-stimulated IEC-6 cells. We found that the TRPM7 inhibitor NS8593 inhibited LPS-induced cytokine production and exhibited an anti-inflammatory effect by alleviating TRPM7-mediated NLRP3 inflammasome activation. Through in-vivo experiments, we found that TRPM7 was involved in the occurrence of NEC, and its inhibitor NS8593 played a certain therapeutic role in the rat model.Our study revealed TRPM7 inhibitors attenuated LPS-induced ROS and reduced the release of pro-inflammatory cytokines. It also exhibited protective effects on the NEC model." @default.
• W4313249108 created "2023-01-06" @default.
• W4313249108 creator A5024506843 @default.
• W4313249108 creator A5026071162 @default.
• W4313249108 creator A5027463542 @default.
• W4313249108 creator A5028908054 @default.
• W4313249108 creator A5028939059 @default.
• W4313249108 creator A5043172053 @default.
• W4313249108 creator A5057287134 @default.
• W4313249108 creator A5076619111 @default.
• W4313249108 date "2022-12-01" @default.
• W4313249108 modified "2023-10-05" @default.
• W4313249108 title "Transient receptor potential melastatin 7 aggravates necrotizing enterocolitis by promoting an inflammatory response in children" @default.
• W4313249108 cites W1154342591 @default.
• W4313249108 cites W1972007800 @default.
• W4313249108 cites W1994473516 @default.
• W4313249108 cites W2028026926 @default.
• W4313249108 cites W2038370680 @default.
• W4313249108 cites W2040664792 @default.
• W4313249108 cites W2077549620 @default.
• W4313249108 cites W2164173823 @default.
• W4313249108 cites W2602492249 @default.
• W4313249108 cites W2766194933 @default.
• W4313249108 cites W2783882235 @default.
• W4313249108 cites W2883313035 @default.
• W4313249108 cites W2884445384 @default.
• W4313249108 cites W2909059466 @default.
• W4313249108 cites W2911076514 @default.
• W4313249108 cites W2943293743 @default.
• W4313249108 cites W2954782052 @default.
• W4313249108 cites W2960998248 @default.
• W4313249108 cites W2996224579 @default.
• W4313249108 cites W3006659767 @default.
• W4313249108 cites W3119135391 @default.
• W4313249108 cites W3119799492 @default.
• W4313249108 cites W3138179690 @default.
• W4313249108 cites W3176626129 @default.
• W4313249108 cites W3203405081 @default.
• W4313249108 cites W3206210215 @default.
• W4313249108 cites W4210732166 @default.
• W4313249108 cites W4235469056 @default.
• W4313249108 doi "https://doi.org/10.21037/tp-22-633" @default.
• W4313249108 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36643673" @default.
• W4313249108 hasPublicationYear "2022" @default.
• W4313249108 type Work @default.
• W4313249108 citedByCount "1" @default.
• W4313249108 crossrefType "journal-article" @default.
• W4313249108 hasAuthorship W4313249108A5024506843 @default.
• W4313249108 hasAuthorship W4313249108A5026071162 @default.
• W4313249108 hasAuthorship W4313249108A5027463542 @default.
• W4313249108 hasAuthorship W4313249108A5028908054 @default.
• W4313249108 hasAuthorship W4313249108A5028939059 @default.
• W4313249108 hasAuthorship W4313249108A5043172053 @default.
• W4313249108 hasAuthorship W4313249108A5057287134 @default.
• W4313249108 hasAuthorship W4313249108A5076619111 @default.
• W4313249108 hasBestOaLocation W43132491081 @default.
• W4313249108 hasConcept C104317684 @default.
• W4313249108 hasConcept C126322002 @default.
• W4313249108 hasConcept C153911025 @default.
• W4313249108 hasConcept C17991360 @default.
• W4313249108 hasConcept C190283241 @default.
• W4313249108 hasConcept C203014093 @default.
• W4313249108 hasConcept C2776415932 @default.
• W4313249108 hasConcept C2776914184 @default.
• W4313249108 hasConcept C2779303187 @default.
• W4313249108 hasConcept C48023723 @default.
• W4313249108 hasConcept C553184892 @default.
• W4313249108 hasConcept C55493867 @default.
• W4313249108 hasConcept C71924100 @default.
• W4313249108 hasConcept C86803240 @default.
• W4313249108 hasConceptScore W4313249108C104317684 @default.
• W4313249108 hasConceptScore W4313249108C126322002 @default.
• W4313249108 hasConceptScore W4313249108C153911025 @default.
• W4313249108 hasConceptScore W4313249108C17991360 @default.
• W4313249108 hasConceptScore W4313249108C190283241 @default.
• W4313249108 hasConceptScore W4313249108C203014093 @default.
• W4313249108 hasConceptScore W4313249108C2776415932 @default.
• W4313249108 hasConceptScore W4313249108C2776914184 @default.
• W4313249108 hasConceptScore W4313249108C2779303187 @default.
• W4313249108 hasConceptScore W4313249108C48023723 @default.
• W4313249108 hasConceptScore W4313249108C553184892 @default.
• W4313249108 hasConceptScore W4313249108C55493867 @default.
• W4313249108 hasConceptScore W4313249108C71924100 @default.
• W4313249108 hasConceptScore W4313249108C86803240 @default.
• W4313249108 hasIssue "12" @default.
• W4313249108 hasLocation W43132491081 @default.
• W4313249108 hasLocation W43132491082 @default.
• W4313249108 hasLocation W43132491083 @default.
• W4313249108 hasOpenAccess W4313249108 @default.
• W4313249108 hasPrimaryLocation W43132491081 @default.
• W4313249108 hasRelatedWork W2041067052 @default.
• W4313249108 hasRelatedWork W2253642286 @default.
• W4313249108 hasRelatedWork W2351699250 @default.
• W4313249108 hasRelatedWork W2363178760 @default.
• W4313249108 hasRelatedWork W2387700717 @default.
• W4313249108 hasRelatedWork W2390313530 @default.
• W4313249108 hasRelatedWork W2390532320 @default.
• W4313249108 hasRelatedWork W2392942585 @default.

• next