Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313251109> ?p ?o ?g. }
- W4313251109 abstract "Centromeres are the specialized regions of the chromosomes that direct faithful chromosome segregation during cell division. Despite their functional conservation, centromeres display features of rapidly evolving DNA and wide evolutionary diversity in size and organization. Previous work found that the noncanonical B-form DNA structures are abundant in the centromeres of several eukaryotic species with a possible implication for centromere specification. Thus far, systematic studies into the organization and function of non-B-form DNA in plants remain scarce. Here, we applied the oat system to investigate the role of non-B-form DNA in centromeres. We conducted chromatin immunoprecipitation sequencing using an antibody to the centromere-specific histone H3 variant (CENH3); this accurately positioned oat centromeres with different ploidy levels and identified a series of centromere-specific sequences including minisatellites and retrotransposons. To define genetic characteristics of oat centromeres, we surveyed the repeat sequences and found that dyad symmetries were abundant in oat centromeres and were predicted to form non-B-DNA structures in vivo. These structures including bent DNA, slipped DNA, Z-DNA, G-quadruplexes, and R-loops were prone to form within CENH3-binding regions. Dynamic conformational changes of predicted non-B-DNA occurred during the evolution from diploid to tetraploid to hexaploid oat. Furthermore, we applied the single-molecule technique of AFM and DNA:RNA immunoprecipitation with deep sequencing to validate R-loop enrichment in oat centromeres. Centromeric retrotransposons exhibited strong associations with R-loop formation. Taken together, our study elucidates the fundamental character of non-B-form DNA in the oat genome and reveals its potential role in centromeres." @default.
- W4313251109 created "2023-01-06" @default.
- W4313251109 creator A5000432967 @default.
- W4313251109 creator A5000510528 @default.
- W4313251109 creator A5008423614 @default.
- W4313251109 creator A5024081154 @default.
- W4313251109 creator A5027463331 @default.
- W4313251109 creator A5027712094 @default.
- W4313251109 creator A5033355508 @default.
- W4313251109 creator A5036948876 @default.
- W4313251109 creator A5039076657 @default.
- W4313251109 creator A5043944448 @default.
- W4313251109 creator A5078163270 @default.
- W4313251109 creator A5086681198 @default.
- W4313251109 date "2022-12-27" @default.
- W4313251109 modified "2023-10-15" @default.
- W4313251109 title "Non–B-form DNA tends to form in centromeric regions and has undergone changes in polyploid oat subgenomes" @default.
- W4313251109 cites W1825949656 @default.
- W4313251109 cites W1975729080 @default.
- W4313251109 cites W1978613333 @default.
- W4313251109 cites W1979557490 @default.
- W4313251109 cites W1983642572 @default.
- W4313251109 cites W1992935264 @default.
- W4313251109 cites W1996423252 @default.
- W4313251109 cites W1999213586 @default.
- W4313251109 cites W2000142302 @default.
- W4313251109 cites W2001211767 @default.
- W4313251109 cites W2006772156 @default.
- W4313251109 cites W2014005472 @default.
- W4313251109 cites W2019196984 @default.
- W4313251109 cites W2040109903 @default.
- W4313251109 cites W2063901213 @default.
- W4313251109 cites W2065085599 @default.
- W4313251109 cites W2073921172 @default.
- W4313251109 cites W2076264335 @default.
- W4313251109 cites W2082330286 @default.
- W4313251109 cites W2088929283 @default.
- W4313251109 cites W2103441770 @default.
- W4313251109 cites W2111913614 @default.
- W4313251109 cites W2112814753 @default.
- W4313251109 cites W2117131162 @default.
- W4313251109 cites W2124638378 @default.
- W4313251109 cites W2128953978 @default.
- W4313251109 cites W2131192238 @default.
- W4313251109 cites W2131271579 @default.
- W4313251109 cites W2143250989 @default.
- W4313251109 cites W2154180530 @default.
- W4313251109 cites W2158789637 @default.
- W4313251109 cites W2159405895 @default.
- W4313251109 cites W2171808845 @default.
- W4313251109 cites W2513735101 @default.
- W4313251109 cites W2590835604 @default.
- W4313251109 cites W2763141754 @default.
- W4313251109 cites W2770128744 @default.
- W4313251109 cites W2770144912 @default.
- W4313251109 cites W2791375414 @default.
- W4313251109 cites W2792156822 @default.
- W4313251109 cites W2939133533 @default.
- W4313251109 cites W2947119785 @default.
- W4313251109 cites W2953980748 @default.
- W4313251109 cites W2956955330 @default.
- W4313251109 cites W2991467126 @default.
- W4313251109 cites W3004073046 @default.
- W4313251109 cites W3095118588 @default.
- W4313251109 cites W3097074770 @default.
- W4313251109 cites W3126554158 @default.
- W4313251109 cites W3134944471 @default.
- W4313251109 cites W3139265997 @default.
- W4313251109 cites W3145811080 @default.
- W4313251109 cites W3163881054 @default.
- W4313251109 cites W3165784772 @default.
- W4313251109 cites W3178472869 @default.
- W4313251109 cites W3196178850 @default.
- W4313251109 cites W3213182660 @default.
- W4313251109 cites W4210804926 @default.
- W4313251109 cites W4221033475 @default.
- W4313251109 cites W4221064448 @default.
- W4313251109 cites W4224234992 @default.
- W4313251109 cites W4225898589 @default.
- W4313251109 cites W4229736484 @default.
- W4313251109 cites W4280509735 @default.
- W4313251109 cites W4280550201 @default.
- W4313251109 cites W4283013446 @default.
- W4313251109 cites W4283165968 @default.
- W4313251109 cites W4285727773 @default.
- W4313251109 doi "https://doi.org/10.1073/pnas.2211683120" @default.
- W4313251109 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36574697" @default.
- W4313251109 hasPublicationYear "2022" @default.
- W4313251109 type Work @default.
- W4313251109 citedByCount "6" @default.
- W4313251109 countsByYear W43132511092023 @default.
- W4313251109 crossrefType "journal-article" @default.
- W4313251109 hasAuthorship W4313251109A5000432967 @default.
- W4313251109 hasAuthorship W4313251109A5000510528 @default.
- W4313251109 hasAuthorship W4313251109A5008423614 @default.
- W4313251109 hasAuthorship W4313251109A5024081154 @default.
- W4313251109 hasAuthorship W4313251109A5027463331 @default.
- W4313251109 hasAuthorship W4313251109A5027712094 @default.
- W4313251109 hasAuthorship W4313251109A5033355508 @default.
- W4313251109 hasAuthorship W4313251109A5036948876 @default.