Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313251695> ?p ?o ?g. }
- W4313251695 abstract "The D,D-transpeptidase activity of penicillin-binding proteins (PBPs) is the well-known primary target of β-lactam antibiotics that block peptidoglycan polymerization. β-lactam-induced bacterial killing involves complex downstream responses whose causes and consequences are difficult to resolve. Here, we use the functional replacement of PBPs by a β-lactam-insensitive L,D-transpeptidase to identify genes essential to mitigate the effects of PBP inactivation by β-lactams in actively dividing bacteria. The functions of the 179 conditionally essential genes identified by this approach extend far beyond L,D-transpeptidase partners for peptidoglycan polymerization to include proteins involved in stress response and in the assembly of outer membrane polymers. The unsuspected effects of β-lactams include loss of the lipoprotein-mediated covalent bond that links the outer membrane to the peptidoglycan, destabilization of the cell envelope in spite of effective peptidoglycan cross-linking, and increased permeability of the outer membrane. The latter effect indicates that the mode of action of β-lactams involves self-promoted penetration through the outer membrane." @default.
- W4313251695 created "2023-01-06" @default.
- W4313251695 creator A5025732669 @default.
- W4313251695 creator A5039721313 @default.
- W4313251695 creator A5048327904 @default.
- W4313251695 creator A5058899747 @default.
- W4313251695 creator A5091210745 @default.
- W4313251695 date "2022-12-27" @default.
- W4313251695 modified "2023-10-09" @default.
- W4313251695 title "Genome-wide identification of genes required for alternative peptidoglycan cross-linking in Escherichia coli revealed unexpected impacts of β-lactams" @default.
- W4313251695 cites W1489615474 @default.
- W4313251695 cites W1576157073 @default.
- W4313251695 cites W1588161749 @default.
- W4313251695 cites W1649027544 @default.
- W4313251695 cites W1924975182 @default.
- W4313251695 cites W1985242603 @default.
- W4313251695 cites W1993341437 @default.
- W4313251695 cites W2014875604 @default.
- W4313251695 cites W2020247237 @default.
- W4313251695 cites W2027403968 @default.
- W4313251695 cites W2028224799 @default.
- W4313251695 cites W2028347256 @default.
- W4313251695 cites W2029207581 @default.
- W4313251695 cites W2037347466 @default.
- W4313251695 cites W2037731504 @default.
- W4313251695 cites W2039045853 @default.
- W4313251695 cites W2063119446 @default.
- W4313251695 cites W2067572953 @default.
- W4313251695 cites W2071973715 @default.
- W4313251695 cites W2099308633 @default.
- W4313251695 cites W2103441770 @default.
- W4313251695 cites W2105475356 @default.
- W4313251695 cites W2105999876 @default.
- W4313251695 cites W2106495559 @default.
- W4313251695 cites W2107194730 @default.
- W4313251695 cites W2107777445 @default.
- W4313251695 cites W2110310254 @default.
- W4313251695 cites W2111967267 @default.
- W4313251695 cites W2116137883 @default.
- W4313251695 cites W2117319600 @default.
- W4313251695 cites W2119481178 @default.
- W4313251695 cites W2120069280 @default.
- W4313251695 cites W2120263648 @default.
- W4313251695 cites W2122385388 @default.
- W4313251695 cites W2137381469 @default.
- W4313251695 cites W21434205 @default.
- W4313251695 cites W2532112416 @default.
- W4313251695 cites W2811259244 @default.
- W4313251695 cites W2884934298 @default.
- W4313251695 cites W2885761488 @default.
- W4313251695 cites W2888315622 @default.
- W4313251695 cites W2895954140 @default.
- W4313251695 cites W2911510427 @default.
- W4313251695 cites W2927944686 @default.
- W4313251695 cites W2959383915 @default.
- W4313251695 cites W2959440892 @default.
- W4313251695 cites W2971158561 @default.
- W4313251695 cites W3003362316 @default.
- W4313251695 cites W3006398942 @default.
- W4313251695 cites W3008011595 @default.
- W4313251695 cites W3016248076 @default.
- W4313251695 cites W3024551994 @default.
- W4313251695 cites W3029430279 @default.
- W4313251695 cites W3048517330 @default.
- W4313251695 cites W3081673076 @default.
- W4313251695 cites W3114674001 @default.
- W4313251695 cites W3134120655 @default.
- W4313251695 cites W3157561306 @default.
- W4313251695 cites W3189217299 @default.
- W4313251695 cites W4205847191 @default.
- W4313251695 cites W4206738935 @default.
- W4313251695 cites W4220971815 @default.
- W4313251695 cites W4247053599 @default.
- W4313251695 cites W4281907276 @default.
- W4313251695 doi "https://doi.org/10.1038/s41467-022-35528-3" @default.
- W4313251695 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36575173" @default.
- W4313251695 hasPublicationYear "2022" @default.
- W4313251695 type Work @default.
- W4313251695 citedByCount "4" @default.
- W4313251695 countsByYear W43132516952023 @default.
- W4313251695 crossrefType "journal-article" @default.
- W4313251695 hasAuthorship W4313251695A5025732669 @default.
- W4313251695 hasAuthorship W4313251695A5039721313 @default.
- W4313251695 hasAuthorship W4313251695A5048327904 @default.
- W4313251695 hasAuthorship W4313251695A5058899747 @default.
- W4313251695 hasAuthorship W4313251695A5091210745 @default.
- W4313251695 hasBestOaLocation W43132516951 @default.
- W4313251695 hasConcept C104317684 @default.
- W4313251695 hasConcept C125235067 @default.
- W4313251695 hasConcept C146587185 @default.
- W4313251695 hasConcept C185592680 @default.
- W4313251695 hasConcept C19655278 @default.
- W4313251695 hasConcept C201663137 @default.
- W4313251695 hasConcept C2776634448 @default.
- W4313251695 hasConcept C2776653525 @default.
- W4313251695 hasConcept C2780183148 @default.
- W4313251695 hasConcept C46371251 @default.
- W4313251695 hasConcept C523546767 @default.
- W4313251695 hasConcept C54355233 @default.
- W4313251695 hasConcept C547475151 @default.