FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313260028> ?p ?o ?g. }

• W4313260028 endingPage "360" @default.
• W4313260028 startingPage "360" @default.
• W4313260028 abstract "Sharka disease, caused by the Plum pox virus (PPV), is one of the most harmful, quarantine viral diseases that affect stone fruit crops. The absence of natural resistance to the virus in stone fruits has become a decisive factor for the use of genetic transformation methods to obtain stable forms. The eIF(iso)4G and eIF(iso)4E genes encode translation initiation factors used in the PPV life cycle. In the presented study, the effect of silencing these genes using the RNA interference method on the resistance of sour cherry rootstock 146-2 plants (Prunus pumila L. x Prunus tomentosa Thunb) to the sharka disease was studied. Two vectors have been created for the genetic transformation of plants, with self-complementary sequences of the eIF(iso)4G and eIF(iso)4E gene fragments. The hairpin expression cassette contains a strong promoter of the peach ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) gene, as well as an intron and terminator of the same gene. We used the pMF1 vector containing recombinase R and a codA-nptII gene which makes it possible to obtain intragenic marker-free plants. A successful genetic transformation was carried out by the AGL0 strain of A. tumefaciens. Whole leaves of shoots cultivated in vitro were used as a source of explants. Eight independent transgenic lines of rootstock 146-2 were obtained in experiments (sixlines with a hairpin to the eIF(iso)4G gene and two lines with a hairpin to the eIF(iso)4E gene). Their status was confirmed by the PCR and Southern blotting. The obtained plants were acclimatized in a greenhouse. The silencing of the eIF(iso)4G and eIF(iso)4E genes in transgenic plants was confirmed by the quantitative PCR. The presence of specific small interfering (si) RNAs was confirmed by the method of Northern blotting. Plants of all transgenic rootstock lines were infected with PPV by the method of grafting with infected buds. Resistance to the PPV infection of the obtained transgenic plants was carried out by using an enzyme immunoassay. The ELISA results showed that silencing the eIF(iso)4G gene did not lead to increased resistance while silencing the eIF(iso)4E factor gene led to increased resistance to the PPV, and the one line’s plants showed no signs of infection for two years after infecting. The work demonstrates a (promising) approach in which the creation of stone cultures resistant to the plum pox virus can be achieved by suppressing the genes of translation initiation factors in clonal rootstocks." @default.
• W4313260028 created "2023-01-06" @default.
• W4313260028 creator A5013210595 @default.
• W4313260028 creator A5021426144 @default.
• W4313260028 creator A5028493191 @default.
• W4313260028 creator A5064118819 @default.
• W4313260028 creator A5065672835 @default.
• W4313260028 creator A5068157537 @default.
• W4313260028 creator A5076053172 @default.
• W4313260028 date "2022-12-26" @default.
• W4313260028 modified "2023-09-27" @default.
• W4313260028 title "Effect of Gene Silencing of Translation Initiation Factors eIF(iso)4G and eIF(iso)4E on Sour Cherry Rootstock Resistance to Sharka Disease" @default.
• W4313260028 cites W1524682667 @default.
• W4313260028 cites W1585722660 @default.
• W4313260028 cites W1600030136 @default.
• W4313260028 cites W1904020976 @default.
• W4313260028 cites W1978439509 @default.
• W4313260028 cites W1981808404 @default.
• W4313260028 cites W1985178609 @default.
• W4313260028 cites W1987454767 @default.
• W4313260028 cites W1993157169 @default.
• W4313260028 cites W1995045055 @default.
• W4313260028 cites W1997004672 @default.
• W4313260028 cites W2006807779 @default.
• W4313260028 cites W2010440035 @default.
• W4313260028 cites W2023680665 @default.
• W4313260028 cites W2024556021 @default.
• W4313260028 cites W2025078829 @default.
• W4313260028 cites W2025625670 @default.
• W4313260028 cites W2035483007 @default.
• W4313260028 cites W2041843243 @default.
• W4313260028 cites W2044201568 @default.
• W4313260028 cites W2044952215 @default.
• W4313260028 cites W2046017813 @default.
• W4313260028 cites W2046407138 @default.
• W4313260028 cites W2051705178 @default.
• W4313260028 cites W2054745484 @default.
• W4313260028 cites W2059531517 @default.
• W4313260028 cites W2065780294 @default.
• W4313260028 cites W2066133211 @default.
• W4313260028 cites W2068571037 @default.
• W4313260028 cites W2072213706 @default.
• W4313260028 cites W2113102010 @default.
• W4313260028 cites W2117388855 @default.
• W4313260028 cites W2146795896 @default.
• W4313260028 cites W2150001658 @default.
• W4313260028 cites W2167871355 @default.
• W4313260028 cites W2187080344 @default.
• W4313260028 cites W2257046203 @default.
• W4313260028 cites W2345123064 @default.
• W4313260028 cites W2508607678 @default.
• W4313260028 cites W2589401389 @default.
• W4313260028 cites W2592681355 @default.
• W4313260028 cites W2776753356 @default.
• W4313260028 cites W2921187207 @default.
• W4313260028 cites W2927718855 @default.
• W4313260028 cites W2951891003 @default.
• W4313260028 cites W2970133961 @default.
• W4313260028 cites W2974963672 @default.
• W4313260028 cites W2977266049 @default.
• W4313260028 cites W2981721459 @default.
• W4313260028 cites W2995622189 @default.
• W4313260028 cites W3009473864 @default.
• W4313260028 cites W3040300652 @default.
• W4313260028 cites W3127457079 @default.
• W4313260028 cites W4293778800 @default.
• W4313260028 cites W4312681577 @default.
• W4313260028 cites W639508829 @default.
• W4313260028 doi "https://doi.org/10.3390/ijms24010360" @default.
• W4313260028 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36613806" @default.
• W4313260028 hasPublicationYear "2022" @default.
• W4313260028 type Work @default.
• W4313260028 citedByCount "0" @default.
• W4313260028 crossrefType "journal-article" @default.
• W4313260028 hasAuthorship W4313260028A5013210595 @default.
• W4313260028 hasAuthorship W4313260028A5021426144 @default.
• W4313260028 hasAuthorship W4313260028A5028493191 @default.
• W4313260028 hasAuthorship W4313260028A5064118819 @default.
• W4313260028 hasAuthorship W4313260028A5065672835 @default.
• W4313260028 hasAuthorship W4313260028A5068157537 @default.
• W4313260028 hasAuthorship W4313260028A5076053172 @default.
• W4313260028 hasBestOaLocation W43132600281 @default.
• W4313260028 hasConcept C104317684 @default.
• W4313260028 hasConcept C204241405 @default.
• W4313260028 hasConcept C2779692143 @default.
• W4313260028 hasConcept C54355233 @default.
• W4313260028 hasConcept C59822182 @default.
• W4313260028 hasConcept C86803240 @default.
• W4313260028 hasConceptScore W4313260028C104317684 @default.
• W4313260028 hasConceptScore W4313260028C204241405 @default.
• W4313260028 hasConceptScore W4313260028C2779692143 @default.
• W4313260028 hasConceptScore W4313260028C54355233 @default.
• W4313260028 hasConceptScore W4313260028C59822182 @default.
• W4313260028 hasConceptScore W4313260028C86803240 @default.
• W4313260028 hasFunder F4320324099 @default.
• W4313260028 hasIssue "1" @default.
• W4313260028 hasLocation W43132600281 @default.
• W4313260028 hasLocation W43132600282 @default.

• next