Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313263367> ?p ?o ?g. }
- W4313263367 abstract "Intrahepatic cholangiocarcinoma (ICC) is a rare malignancy with a high prevalence in China. This study aimed to characterize the ICC tissues' bacterial metagenomics signature and explore its antitumor potential for cancer. In this study, 16S rRNA sequencing was carried out on 99 tissues to characterize the features of intratumoral microbiota, followed by single-cell RNA sequencing (scRNA-seq) and multilevel validation. The presence of microbial DNA in tissues was determined using staining, fluorescence in situ hybridization (FISH), and transmission electron microscopy (TEM). A Gram-positive aerobic bacterium, identified as Staphylococcus capitis, was cultured from fresh tissues. Meanwhile, scRNA-seq showed that intratumoral bacteria could be present in multiple cell types. Using 16S rRNA sequencing, we identified a total of 2,320,287 high-quality reads corresponding to 4,594 OTU (operational taxonomic units) sequences. The most abundant bacterial orders include Burkholderiales, Pseudomonadales, Xanthomonadales, Bacillales and Clostridiales. Alpha and Beta diversity analysis revealed specific features in different tissues. In addition, the content of Paraburkholderia fungorum was significantly higher in the paracancerous tissues and negatively correlated with CA199 (Carbohydrate antigen199) levels. The results of in vitro and in vivo experiments suggest that P. fungorum possesses an antitumor activity against tumors. Metabolomics and transcriptomics showed that P. fungorum could inhibit tumor growth through alanine, aspartate and glutamate metabolism. We determined the characteristic profile of the intratumoral microbiota and the antitumor effect of P. fungorum in ICC." @default.
- W4313263367 created "2023-01-06" @default.
- W4313263367 creator A5015792149 @default.
- W4313263367 creator A5019202280 @default.
- W4313263367 creator A5022268304 @default.
- W4313263367 creator A5025087223 @default.
- W4313263367 creator A5029634246 @default.
- W4313263367 creator A5030401096 @default.
- W4313263367 creator A5030949828 @default.
- W4313263367 creator A5033085089 @default.
- W4313263367 creator A5035556419 @default.
- W4313263367 creator A5035664283 @default.
- W4313263367 creator A5053582934 @default.
- W4313263367 creator A5053760178 @default.
- W4313263367 creator A5060470951 @default.
- W4313263367 creator A5060973548 @default.
- W4313263367 creator A5064590706 @default.
- W4313263367 creator A5065811893 @default.
- W4313263367 creator A5075877965 @default.
- W4313263367 creator A5076640232 @default.
- W4313263367 creator A5079615657 @default.
- W4313263367 date "2022-12-23" @default.
- W4313263367 modified "2023-10-18" @default.
- W4313263367 title "Intratumor microbiome features reveal antitumor potentials of intrahepatic cholangiocarcinoma" @default.
- W4313263367 cites W1934236512 @default.
- W4313263367 cites W1973872654 @default.
- W4313263367 cites W1984916935 @default.
- W4313263367 cites W1997981441 @default.
- W4313263367 cites W1999906527 @default.
- W4313263367 cites W2034285706 @default.
- W4313263367 cites W2035378093 @default.
- W4313263367 cites W2036897871 @default.
- W4313263367 cites W2051567523 @default.
- W4313263367 cites W2055125523 @default.
- W4313263367 cites W2071168599 @default.
- W4313263367 cites W2072970694 @default.
- W4313263367 cites W2073924079 @default.
- W4313263367 cites W2078599166 @default.
- W4313263367 cites W2088833470 @default.
- W4313263367 cites W2108718991 @default.
- W4313263367 cites W2111647009 @default.
- W4313263367 cites W2132406938 @default.
- W4313263367 cites W2133856765 @default.
- W4313263367 cites W2151350595 @default.
- W4313263367 cites W2152885278 @default.
- W4313263367 cites W2157107905 @default.
- W4313263367 cites W2164386968 @default.
- W4313263367 cites W2164906049 @default.
- W4313263367 cites W2311203695 @default.
- W4313263367 cites W2317340981 @default.
- W4313263367 cites W2345658906 @default.
- W4313263367 cites W2346481496 @default.
- W4313263367 cites W2559588208 @default.
- W4313263367 cites W2625068954 @default.
- W4313263367 cites W2768799378 @default.
- W4313263367 cites W2784137104 @default.
- W4313263367 cites W2793947535 @default.
- W4313263367 cites W2797068438 @default.
- W4313263367 cites W2888547849 @default.
- W4313263367 cites W2900629010 @default.
- W4313263367 cites W2907514116 @default.
- W4313263367 cites W2949177718 @default.
- W4313263367 cites W2950168445 @default.
- W4313263367 cites W2952463830 @default.
- W4313263367 cites W2955245896 @default.
- W4313263367 cites W2967450000 @default.
- W4313263367 cites W2990618091 @default.
- W4313263367 cites W2990919242 @default.
- W4313263367 cites W2997490540 @default.
- W4313263367 cites W2999689705 @default.
- W4313263367 cites W3017206405 @default.
- W4313263367 cites W3027966102 @default.
- W4313263367 cites W3030441098 @default.
- W4313263367 cites W3030942202 @default.
- W4313263367 cites W3033473768 @default.
- W4313263367 cites W3099584446 @default.
- W4313263367 cites W3112963314 @default.
- W4313263367 cites W3118017075 @default.
- W4313263367 cites W3124058635 @default.
- W4313263367 cites W3137683977 @default.
- W4313263367 cites W3138215020 @default.
- W4313263367 cites W3216873694 @default.
- W4313263367 cites W4240924657 @default.
- W4313263367 cites W4280578513 @default.
- W4313263367 doi "https://doi.org/10.1080/19490976.2022.2156255" @default.
- W4313263367 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36563106" @default.
- W4313263367 hasPublicationYear "2022" @default.
- W4313263367 type Work @default.
- W4313263367 citedByCount "5" @default.
- W4313263367 countsByYear W43132633672023 @default.
- W4313263367 crossrefType "journal-article" @default.
- W4313263367 hasAuthorship W4313263367A5015792149 @default.
- W4313263367 hasAuthorship W4313263367A5019202280 @default.
- W4313263367 hasAuthorship W4313263367A5022268304 @default.
- W4313263367 hasAuthorship W4313263367A5025087223 @default.
- W4313263367 hasAuthorship W4313263367A5029634246 @default.
- W4313263367 hasAuthorship W4313263367A5030401096 @default.
- W4313263367 hasAuthorship W4313263367A5030949828 @default.
- W4313263367 hasAuthorship W4313263367A5033085089 @default.
- W4313263367 hasAuthorship W4313263367A5035556419 @default.