FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313280088> ?p ?o ?g. }

• W4313280088 endingPage "262" @default.
• W4313280088 startingPage "254" @default.
• W4313280088 abstract "Clinical tuberculosis (TB) screening and diagnosis are crucial for controlling the spread of this life-threatening infectious disease. In this work, a novel, rapid, and simple colorimetric detection platform for TB was developed based on a quantum dot-based nanobeacon (QD-NB) and multicomponent nucleic acid enzyme (MNAzyme). In the presence of target DNA (IS1081 gene fragment), the recombinase polymerase amplification (RPA) was performed and the amplicons were chemically DNA-denatured and then subjected to MNAzyme reaction. RNA-cleaving MNAzyme assembly included the recognition of target DNA and hybridization with a QD-NB fluorescence probe. Under the addition of Mg2+, the RNA-containing QD-NB as a cleavable substrate could be broken into two DNA fragments, leading to green fluorescence release due to their departure from a black hole quencher (BHQ2). The TB detection could be achieved with the naked eye under a portable and inexpensive UV flashlight. Our results demonstrated that QD-NB-based MNAzyme colorimetric assays improved the detection sensitivity by 1 order of magnitude compared with the detection using RPA. The limit of detection (LOD) of the visual reading was as low as 2 copies/μL (3.3 amol/L). Excellent specificity and reproducibility could also be achieved. Furthermore, the practical application of the colorimetric method for TB diagnosis was verified by 36 clinical TB patients and 20 healthy individuals. The developed QD-NB-based MNAzyme colorimetric assays provided a rapid, convenient, sensitive, and accurate alternative for clinical TB screening and diagnosis." @default.
• W4313280088 created "2023-01-06" @default.
• W4313280088 creator A5015162536 @default.
• W4313280088 creator A5022407487 @default.
• W4313280088 creator A5024027469 @default.
• W4313280088 creator A5033714897 @default.
• W4313280088 creator A5048090419 @default.
• W4313280088 creator A5086214932 @default.
• W4313280088 date "2022-12-29" @default.
• W4313280088 modified "2023-09-27" @default.
• W4313280088 title "A Multicomponent Nucleic Acid Enzyme-Cleavable Quantum Dot Nanobeacon for Highly Sensitive Diagnosis of Tuberculosis with the Naked Eye" @default.
• W4313280088 cites W1974637385 @default.
• W4313280088 cites W2044236646 @default.
• W4313280088 cites W2048522427 @default.
• W4313280088 cites W2083884966 @default.
• W4313280088 cites W2096961704 @default.
• W4313280088 cites W2099884041 @default.
• W4313280088 cites W2100908250 @default.
• W4313280088 cites W2105784131 @default.
• W4313280088 cites W2170476107 @default.
• W4313280088 cites W2259212906 @default.
• W4313280088 cites W2317651422 @default.
• W4313280088 cites W2418180773 @default.
• W4313280088 cites W2516091697 @default.
• W4313280088 cites W2582958454 @default.
• W4313280088 cites W2737860676 @default.
• W4313280088 cites W2898319406 @default.
• W4313280088 cites W2903624028 @default.
• W4313280088 cites W2964190341 @default.
• W4313280088 cites W2973231660 @default.
• W4313280088 cites W2995076391 @default.
• W4313280088 cites W2998918328 @default.
• W4313280088 cites W3011496369 @default.
• W4313280088 cites W3011542894 @default.
• W4313280088 cites W3024978864 @default.
• W4313280088 cites W3090882943 @default.
• W4313280088 cites W3091127702 @default.
• W4313280088 cites W3108557870 @default.
• W4313280088 cites W3111795209 @default.
• W4313280088 cites W3119079277 @default.
• W4313280088 cites W3123655292 @default.
• W4313280088 cites W3135237076 @default.
• W4313280088 cites W3135682442 @default.
• W4313280088 cites W3137328652 @default.
• W4313280088 cites W3160707723 @default.
• W4313280088 cites W3164006366 @default.
• W4313280088 cites W3167751787 @default.
• W4313280088 cites W3198758083 @default.
• W4313280088 cites W3201491582 @default.
• W4313280088 cites W3214168639 @default.
• W4313280088 cites W4211104832 @default.
• W4313280088 cites W4225165960 @default.
• W4313280088 cites W4226381515 @default.
• W4313280088 cites W4283361562 @default.
• W4313280088 cites W4283367411 @default.
• W4313280088 cites W4283693227 @default.
• W4313280088 cites W4285730698 @default.
• W4313280088 doi "https://doi.org/10.1021/acssensors.2c02114" @default.
• W4313280088 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/36579361" @default.
• W4313280088 hasPublicationYear "2022" @default.
• W4313280088 type Work @default.
• W4313280088 citedByCount "2" @default.
• W4313280088 countsByYear W43132800882023 @default.
• W4313280088 crossrefType "journal-article" @default.
• W4313280088 hasAuthorship W4313280088A5015162536 @default.
• W4313280088 hasAuthorship W4313280088A5022407487 @default.
• W4313280088 hasAuthorship W4313280088A5024027469 @default.
• W4313280088 hasAuthorship W4313280088A5033714897 @default.
• W4313280088 hasAuthorship W4313280088A5048090419 @default.
• W4313280088 hasAuthorship W4313280088A5086214932 @default.
• W4313280088 hasConcept C104317684 @default.
• W4313280088 hasConcept C119128265 @default.
• W4313280088 hasConcept C121332964 @default.
• W4313280088 hasConcept C139713532 @default.
• W4313280088 hasConcept C153911025 @default.
• W4313280088 hasConcept C158531540 @default.
• W4313280088 hasConcept C185592680 @default.
• W4313280088 hasConcept C24107716 @default.
• W4313280088 hasConcept C43617362 @default.
• W4313280088 hasConcept C49105822 @default.
• W4313280088 hasConcept C552990157 @default.
• W4313280088 hasConcept C55493867 @default.
• W4313280088 hasConcept C60635243 @default.
• W4313280088 hasConcept C62520636 @default.
• W4313280088 hasConcept C8185291 @default.
• W4313280088 hasConcept C86803240 @default.
• W4313280088 hasConcept C91881484 @default.
• W4313280088 hasConceptScore W4313280088C104317684 @default.
• W4313280088 hasConceptScore W4313280088C119128265 @default.
• W4313280088 hasConceptScore W4313280088C121332964 @default.
• W4313280088 hasConceptScore W4313280088C139713532 @default.
• W4313280088 hasConceptScore W4313280088C153911025 @default.
• W4313280088 hasConceptScore W4313280088C158531540 @default.
• W4313280088 hasConceptScore W4313280088C185592680 @default.
• W4313280088 hasConceptScore W4313280088C24107716 @default.
• W4313280088 hasConceptScore W4313280088C43617362 @default.
• W4313280088 hasConceptScore W4313280088C49105822 @default.
• W4313280088 hasConceptScore W4313280088C552990157 @default.

• next