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- W4313289879 abstract "Triterpenoids are the major bioactive constituents of the edible and medicinal mushroom Sanghuangporus baumii. The polyunsaturated fatty acid is an excellent stimulator that promotes the production of triterpenoids significantly. In this study, the detailed underlying mechanisms between linolenic acid and triterpenoids production were further investigated. GC-MS analysis, the release of cell constituents, SEM observation and MDA content determination showed the changes in cell membrane composition, permeability and morphology. A higher unsaturated/saturated fatty acid ratio, better membrane permeability and fluidity, and enhanced degree of mycelial wrinkles and lipid peroxidation were observed. Furthermore, the expression levels and enzyme activities of HMGS, HMGR, FPPS, SQS and SE were significantly upregulated by adding linolenic acid at specific period. All mechanism explorations indicated that the effects of exogenous linolenic acid on triterpenoids production were tightly correlated with substrate supply, change of cell membrane and metabolic regulation of triterpenoids biosynthetic pathway. Graphical abstractDownload : Download high-res image (181KB)Download : Download full-size image" @default.
- W4313289879 created "2023-01-06" @default.
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- W4313289879 date "2023-02-01" @default.
- W4313289879 modified "2023-10-12" @default.
- W4313289879 title "The mechanistic study of adding polyunsaturated fatty acid to promote triterpenoids production in submerged fermentation of Sanghuangporus baumii" @default.
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- W4313289879 doi "https://doi.org/10.1016/j.bej.2022.108800" @default.
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