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- W4313292010 abstract "Improved; fast; and specific detection of pathogens has always been of great importance; more so with the increase of human population and human interaction. In this work we investigate the application of metal oxide nanoparticles (ZnO; Fe 2 O 3 ; and TiO 2 ) in the detection of the pathogen Salmonella enteritidis using real-time quantitative PCR (qPCR). The nanoparticles were synthesized and characterized; and then they were added at different concentrations to qPCR for Salmonella enteritidis detection. qPCR provides numerical data such as threshold cycle (Ct); efficiency; and DNA yield which make comparing the different tested conditions easier and more accurate. It has been observed that adding all three types of NPs at an optimum concentration 4×10 −11 M for ZnO NPs, and 4×10 −9 for Fe 2 O 3 and TiO 2 nanoparticles has led to increasing the efficiency of the reaction to 100% and to lowering the threshold cycle value by up to 6.6 for ZnO nanoparticles; hence increasing the DNA yield of the reaction; and lowering the detection time of the pathogen by up to 50%." @default.
- W4313292010 created "2023-01-06" @default.
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- W4313292010 date "2022-08-01" @default.
- W4313292010 modified "2023-10-17" @default.
- W4313292010 title "Enhancement of <i>Salmonella</i> Enteritidis Detection Using Nanoparticle-Assisted Real-Time Polymerase Chain Reaction" @default.
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- W4313292010 doi "https://doi.org/10.1166/jbn.2022.3401" @default.
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