Matches in SemOpenAlex for { <https://semopenalex.org/work/W4313308878> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W4313308878 endingPage "73" @default.
- W4313308878 startingPage "65" @default.
- W4313308878 abstract "Abstract Rabbits were immunized by intraperitoneal injections of DNP-Ascaris antigen precipitated with aluminum hydroxide gel to form reaginic antibodies. Mesenteric lymph node cells from the primed rabbits were stimulated in vitro by incubating with homologous antigen for 24 hr and then cultured for 6 days in the absence of antigen. Measurements of IgG and reaginic antibodies in culture fluids showed that the lymph node cells from the rabbits which formed reaginic antibodies after primary immunization produced IgG antibodies. In vitro reaginic antibody formation was generally observed when donors of lymph node cells showed both primary and secondary reaginic antibody responses after the primary and booster injections. The titer of reaginic antibody in the culture fluid, as determined by the PCA reaction, did not correlate with the concentration of IgG antibodies in the fluid, or with the reaginic antibody titer of the serum of donors at the time of sacrifice. The molecular size of reaginic antibody in the culture fluids was intermediate between those of IgM and IgG antibodies. The skin-sensitizing activity of the antibody was completely lost by treatment with 0.01 M dithiothreitol followed by alkylation and slightly diminished after heating at 56°C for 2 hr. Such properties of the reaginic antibodies in the culture fluid were comparable to those of IgE antibodies in the serum. It was found that a removal of most of the adherent cells in lymph node cell suspension before antigen stimulation in vitro did not have a significant effect on the formation of IgG, IgM and IgE antibodies in the culture. An addition of monospecific anti-IgG with antigen for in vitro stimulation suppressed in vitro IgG and IgM antibody formation but not the IgE antibody formation." @default.
- W4313308878 created "2023-01-06" @default.
- W4313308878 creator A5082294819 @default.
- W4313308878 creator A5090153286 @default.
- W4313308878 date "1972-07-01" @default.
- W4313308878 modified "2023-10-18" @default.
- W4313308878 title "Regulation of Antibody Response in Vitro" @default.
- W4313308878 doi "https://doi.org/10.4049/jimmunol.109.1.65" @default.
- W4313308878 hasPublicationYear "1972" @default.
- W4313308878 type Work @default.
- W4313308878 citedByCount "13" @default.
- W4313308878 countsByYear W43133088782016 @default.
- W4313308878 crossrefType "journal-article" @default.
- W4313308878 hasAuthorship W4313308878A5082294819 @default.
- W4313308878 hasAuthorship W4313308878A5090153286 @default.
- W4313308878 hasConcept C141105273 @default.
- W4313308878 hasConcept C147483822 @default.
- W4313308878 hasConcept C153911025 @default.
- W4313308878 hasConcept C159654299 @default.
- W4313308878 hasConcept C185592680 @default.
- W4313308878 hasConcept C202751555 @default.
- W4313308878 hasConcept C203014093 @default.
- W4313308878 hasConcept C2780849966 @default.
- W4313308878 hasConcept C32611913 @default.
- W4313308878 hasConcept C55493867 @default.
- W4313308878 hasConcept C86803240 @default.
- W4313308878 hasConcept C91523082 @default.
- W4313308878 hasConceptScore W4313308878C141105273 @default.
- W4313308878 hasConceptScore W4313308878C147483822 @default.
- W4313308878 hasConceptScore W4313308878C153911025 @default.
- W4313308878 hasConceptScore W4313308878C159654299 @default.
- W4313308878 hasConceptScore W4313308878C185592680 @default.
- W4313308878 hasConceptScore W4313308878C202751555 @default.
- W4313308878 hasConceptScore W4313308878C203014093 @default.
- W4313308878 hasConceptScore W4313308878C2780849966 @default.
- W4313308878 hasConceptScore W4313308878C32611913 @default.
- W4313308878 hasConceptScore W4313308878C55493867 @default.
- W4313308878 hasConceptScore W4313308878C86803240 @default.
- W4313308878 hasConceptScore W4313308878C91523082 @default.
- W4313308878 hasIssue "1" @default.
- W4313308878 hasLocation W43133088781 @default.
- W4313308878 hasOpenAccess W4313308878 @default.
- W4313308878 hasPrimaryLocation W43133088781 @default.
- W4313308878 hasRelatedWork W1589286368 @default.
- W4313308878 hasRelatedWork W1972725327 @default.
- W4313308878 hasRelatedWork W1973692620 @default.
- W4313308878 hasRelatedWork W2048230388 @default.
- W4313308878 hasRelatedWork W2081175840 @default.
- W4313308878 hasRelatedWork W2359730144 @default.
- W4313308878 hasRelatedWork W2381957667 @default.
- W4313308878 hasRelatedWork W2388278648 @default.
- W4313308878 hasRelatedWork W2409452022 @default.
- W4313308878 hasRelatedWork W4313366611 @default.
- W4313308878 hasVolume "109" @default.
- W4313308878 isParatext "false" @default.
- W4313308878 isRetracted "false" @default.
- W4313308878 workType "article" @default.